Electrochemical Detection of Epinephrine in The Presence of Ascorbic Acid at Poly (p-Aminobenzene Sulfonic Acid) Modified Sensor

Şevket Zişan Bilge Yağcı; Ebru Kuyumcu Savan; Gamze Erdoğdu

doi:10.17776/csj.426874

TR EN

Poli (p-Aminobenzen Sülfonik Asit) Modifiye Sensöründe Askorbik Asit Varlığında Epinefrinin Elektrokimyasal Tayini

Öz

Poli (p-aminobenzen sülfonik asit, ABSA) modifiye sensör ile askorbik asit varlığında epinefrinin (EP) hızlı, güvenilir ve duyarlı bir şekilde seçici ve eş zamanlı voltametrik tayini gerçekleştirildi. Camsı karbon elektrot, ABSA ile 0.1 M KCl çözeltisi içerisinde çevrimli voltametri tekniğiyle başarılı bir şekilde modifiye edildi. Polimerik ince film ile modifiye edilen sensör, EP ve askorbik asitin (AA) yükseltgenmesine karşı mükemmel elektro-katalitik etki göstermiştir. Sonuçlar, her iki türün oksidasyon potansiyeli ve oksidasyon pik akım yanıtlarının önemli derecede iyileştiğini göstermiştir. Modifiye sensör, optimum koşullar altında EP tayinine karşı 5.0–53.2 µM derişim aralığında 50 nM tayin sınırı ile mükemmel cevap göstermiştir. Farmasötik preparatta ve kan serumunda gerçekleştirilen gerçek numune analizlerinde geri kazanım değerleri % 98.4 - 77.3 di. Elde edilen sonuçlar, modifiye sensörün AA varlığında EP’in saptanmasında uygulanabileceğini göstermiştir. Önerilen sensörün, yüksek seçicilik, tekrarlanabilirlik, tekrarüretilebilirlik ve uzun-süreli kararlılık özelliklerinden ve farmasötik ve biyolojik örneklerde elde edilen yüksek geri kazanım değerlerinden dolayı rutin analizlerde uygulanabilecek olması umut vericidir.

Anahtar Kelimeler

Epinefrin,p-aminobenzen sülfonik asit,sensör,voltametri

Electrochemical Detection of Epinephrine in The Presence of Ascorbic Acid at Poly (p-Aminobenzene Sulfonic Acid) Modified Sensor

Abstract

In this study, a rapid, reliable, selective and sensitive simultaneous voltammetric determination of Epinephrine (EP) in the presence of ascorbic acid at poly (p-aminobenzene sulphonic acid, ABSA) modified sensor was aimed. The glassy carbon electrode was successfully modified with ABSA in 0.1 M KCl solution by the cycling voltammetry technique. The sensor, modified with a polymeric thin film, showed excellent electrocatalytic activity against the oxidation of EP and ascorbic acid (AA). The results showed that the oxidation potential and current responses of EP and AA improved significantly. The modified sensor showed excellent response with limit of detection as 50 nM in the determination of EP at the 5.0 - 53.2 μM concentration range under optimum conditions. In real sample analyzes performed in pharmaceutical preparation and blood serum, recovery values were 77.3%-98.4%. The results obtained show that the modified sensor can be applied to the detection of EP in the presence of AA. The proposed sensor is promising for routine analysis because of its high selectivity, reproducibility, reproducibility and long-term stability characteristics and high recovery values obtained in pharmaceutical and biological samples.

Keywords

Epinephrine,p-aminobenzene sulfonic acid,sensor,voltammetry,voltammetry

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Şevket Zişan Bilge Yağcı
0000-0002-6064-3177
Türkiye

Ebru Kuyumcu Savan
0000-0002-8851-0907
Türkiye

Gamze Erdoğdu ^*
0000-0002-8114-6946
Türkiye

Publication Date

December 24, 2018

Submission Date

May 25, 2018

Acceptance Date

December 6, 2018

Published in Issue

Year 2018 Volume: 39 Number: 4

DOI

https://doi.org/10.17776/csj.426874

IZ

https://izlik.org/JA73CY73FF

Cite

RIS / Bibtex

APA

Yağcı, Ş. Z. B., Kuyumcu Savan, E., & Erdoğdu, G. (2018). Electrochemical Detection of Epinephrine in The Presence of Ascorbic Acid at Poly (p-Aminobenzene Sulfonic Acid) Modified Sensor. Cumhuriyet Science Journal, 39(4), 1025-1035. https://doi.org/10.17776/csj.426874