Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads

Haydar Altınok

doi:10.17776/csj.418897

EN TR

Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads

Abstract

Laccase enzyme (L) obtained from Tramates versicolor was entrapped into polyvinyl alcohol–calcium alginate (PVA-CaAlj) beads. Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) values were found to be 1.70x10^-2 mM and 2.08x10^-3 mM.min^-1 for free enzyme respectively. Km and Vmax values were found as 2.87x10^-2 mM and 5.30 x 10^-3 mM.min^-1 for entrapped enzymes respectively. Optimum pH was determined as 5.0 and 6.0 and optimum temperature determined as 40^oC and 45^oC for free laccase and entrapped laccase respectively. After 30 days of storage at 4 ^oC free laccase retained 60 % of its original activity. Also after 30 days of storage at 4 ^oC, entrapped enzymes were retained 85 % its original activity. Immobilized enzyme was used repeatedly 10 times, were retained 75% of its original activities.

Keywords

Laccase,immobilization,polyvinyl alcohol–calcium alginate,entrapment

References

[1]. Laurent N., Haddoub R., Flitsch SL., Enzyme catalysis on solid surfaces, Trends Biotechnol., 26 (2008) 328–337.
[2]. Tischer W. and Kasche V., Immobilized enzymes: crystals or carriers, Trends Biotechnol., 17 (1999) 326–335.
[3]. Pang R., Li M., Zhang C., Degradation of phenolic compounds by laccase immobilized on carbon nanomaterials: Diffusional limitation investigation, Talanta., 131(2015) 38-45.
[4]. D’annibale A., Stazi S.R., Vinciguerra V., Mattia E.D., Sermanni G.G., Characterization of immobilized laccase from Lentinula edodes and its use in olive-mill wastewater treatment, Process Biochem., 34 (1999) 697–706.
[5]. D’annibale A., Stazi S.R., Vinciguerra V., Sermanni G., Oxirane-immobilized Lentinula edodes laccase: stability and phenolics removal efficiency in olive mill wastewater, J Biotechnol., 77 (2000) 265–273.
[6]. Jiang D.S., Long S.Y., Huang J., Xıao H.Y., Zhou J.Y., Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres, Biochem. Eng. J., 25 (2005) 15–23.
[7]. Durán N., Rosa M.A., D’annibale A., Gianfreda L., Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review, Enzyme Microb. Tec., 31(2002) 907–931.
[8]. Jolivalta C., Brenon S., Caminade E., Mougin C., Pontié M., Immobilization of laccase from Trametes versicolor on a modified PVDF microfiltration membrane: characterization of the grafted support and application in removing a phenylurea pesticide in wastewater, J. Membrane Sci., 180(2000) 103–113.

[9]. Wan Y., Lu R., Xiao L., Du Y., Miyakoshi T., Chen C., Knill C., Kennedy J., Effects of organic solvents on the activity of free and immobilised laccase from Rhus vernicifera, Int. J. Biol. Macromol., 47 (2010) 488–495.
[10]. Rotkova J., Sulakova R., Korecka L., Zdrazilova P., Jandova M., Lenfeld J., Horak D., Bilkova Z., Laccase immobilized on magnetic carriers for biotechnology applications, J. Magn. Mater., 321 (2009) 1335–1340.
[11]. Curulli A., Cusma A., Kaciulis S., Padeletti G., Pandolfi L., Valentini F., Vitocelli M., Immobilization of GOD and HRP enzyme on nanostructured substrates, Surf. Interface Anal., 38 (2006) 478–481.
[12]. Gokgoz M. and Altinok H., Immobilization of laccase on polyacrylamide and polyacrylamide - κ - carragennan-based semi-interpenetrating polymer networks, Artificial Cells, Blood Substitutes, and Biotechnology, 40 (2012) 326–330.
[13]. Yamak O., Kalkan N.A., Aksoy S., Altinok H., Hasirci N., Semi-interpenetrating polymer networks (semi-IPNs) for entrapment of laccase and their use in Acid Orange 52 decolorization, Process Biochemistry, 44 (2009) 440-445.
[14]. Koklukaya S.Z., Sezer S., Aksoy S., Hasirci N., Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization, Biotechnology and Applied Biochemistry, 63(5) (2016) 699-707.
[15]. Makas Y.G. , Kalkan N.A., Aksoy S., Altinok H., Hasirci N., Immobilization of laccase in -carrageenan based semi-interpenetrating polymer Networks, Journal of Biotechnology, 148 (2010) 216–220.
[16]. Leonowicz A. and Grzywnowicz K., Quantitative estimation of laccase forms in some white rot fungi using syringaldazine as a substrate, Enzyme Microb. Tech., 3 (1981) 55–58.
[17]. Lante A., Crapisi A., Krastanov A., Spettoli P., Biodegradation of phenols by laccase immobilised in a membrane reactor, Process Biochem., 36 (2000) 51–58.
[18]. Al-Adhami A.J.H., Bryjak J., Markiewicz B.G., Chozch W.P., Immobilization of woodrotting fungi laccases on modified cellulose and acrylic carriers, Process Biochem., 37 (2002)1387–1394.
[19]. Dodor D.E., Hwang H., Ekunwe S., Oxidation of anthracene and benzo[a]pyrene by immobilized laccase from Trametes versicolor, Enzyme Microb. Tech., 35 (2004) 210–217.
[20]. Fang H., Huang J., Ding L., Li M., Chen Z., Preparation of magnetic Chitosan nanoparticles and immobilization of Laccase, Journal of Wuhan University of Technology-Mater. Sci. Ed., 24 (2009) 42-47.
[21]. Yang W.Y., Min D.Y., Xiao S.W., Jin L., Rong L., Tetsuo M., Bo C., Immobilization and characterization of laccase from Chinese Rhus vernicifera on modified chitosan, Process Biochem., 41 (2006) 1378-1382.
[22]. Lineweaver H. and Burk D.J., The determination of enzyme dissociation constant, Am. Chem. Soc., 56 (1934) 658–666.
[23]. Xiao H., Huang J., Liu C., Jiang D., Immobilization of Laccase on amine-terminated magnetic nano-composite by glutaraldehyde crosslinking method, T. Nonferr. Metal Soc., 16 (2006) 414-418.
[24]. Zamora P. P., Pereira M. C., Tiburtius R. L., Rosa M.A., Minussi C.R., Duran N., Decolarization of reactive dyes by immobilized Laccase, Appl. Catal B-Environ ., 42 (2003) 131-144.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Haydar Altınok
Türkiye

Publication Date

September 30, 2018

Submission Date

April 26, 2018

Acceptance Date

September 12, 2018

Published in Issue

Year 2018 Volume: 39 Number: 3

DOI

https://doi.org/10.17776/csj.418897

IZ

https://izlik.org/JA75FU62HR

Cite

RIS / Bibtex

APA

Altınok, H. (2018). Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads. Cumhuriyet Science Journal, 39(3), 688-693. https://doi.org/10.17776/csj.418897

Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads

Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads

Abstract

Keywords

Lakkazın poli (Vinil Alkol) -Kalsiyum Aljinat Kürelerine İmmobilizasyonu

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite