Investigation of the Inhibition Effects of Some Metal Ions on Glutathione Reductase Enzyme From Japanese Quail (Coturnix coturnix japonica) Liver

Yusuf Temel; Sarkat Salim Mohammed Taher; Mohammad Ahmad Hamza; İbrahim Hamdi Shafeeq; Ümit Muhammet Koçyiğit; Mehmet Çiftçi

doi:10.17776/csj.414680

EN TR

Investigation of the Inhibition Effects of Some Metal Ions on Glutathione Reductase Enzyme From Japanese Quail (Coturnix coturnix japonica) Liver

Abstract

In the present study, the inhibition effect of some heavy metal ions on glutathione reductase (GR) enzyme which has important functions in metabolism, was investigated. For this purpose, GR enzyme was purified with 19.33 EU/mg specific activity, a yield of 14.06% and 128.8-fold from quail liver by using ammonium sulphate precipitation and 2ʹ, 5ʹ-ADP Sepharose 4B affinity chromatography. To check the purity of GR enzyme, sodium dodecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The activity of the GR enzyme was measured spectrophotometrically at 340 nm according to the method of Carlberg and Mannervik. Then, the inhibitory effects of different heavy metal ions (Co ²⁺, Zn ²⁺, Pb ²⁺, Fe ²⁺, Cd ²⁺, Al ³⁺ and Fe ³⁺) on the activity of GR enzyme were examined under in vitro conditions. For the GR enzyme from quail liver tissue, heavy metal concentrations (IC₅₀) were obtained in which 50% of GR enzyme activity was inhibited in vitro conditions. Finally, K_i values for these metal ions were calculated from the Lineweaver-Burk plots.

Keywords

Glutathion Reductase,Enzyme,Purification,Quail,Inhibition,Liver

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Yusuf Temel

Sarkat Salim Mohammed Taher

Mohammad Ahmad Hamza

İbrahim Hamdi Shafeeq

Ümit Muhammet Koçyiğit ^*
Türkiye

Mehmet Çiftçi

Publication Date

September 30, 2018

Submission Date

April 12, 2018

Acceptance Date

August 27, 2018

Published in Issue

Year 2018 Volume: 39 Number: 3

DOI

https://doi.org/10.17776/csj.414680

IZ

https://izlik.org/JA63PE92CE

Cite

RIS / Bibtex

APA

Temel, Y., Taher, S. S. M., Hamza, M. A., Shafeeq, İ. H., Koçyiğit, Ü. M., & Çiftçi, M. (2018). Investigation of the Inhibition Effects of Some Metal Ions on Glutathione Reductase Enzyme From Japanese Quail (Coturnix coturnix japonica) Liver. Cumhuriyet Science Journal, 39(3), 679-687. https://doi.org/10.17776/csj.414680

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Pathogens

https://doi.org/10.3390/pathogens14060593

Investigation of the Inhibition Effects of Some Metal Ions on Glutathione Reductase Enzyme From Japanese Quail (Coturnix coturnix japonica) Liver

Investigation of the Inhibition Effects of Some Metal Ions on Glutathione Reductase Enzyme From Japanese Quail (Coturnix coturnix japonica) Liver

Abstract

Keywords

Bazı Metal İyonlarının Japon Bıldırcın (Coturnix coturnix japonica) Karaciğer Glutatyon Redüktaz Enzimi Aktivitesi Üzerine İnhibisyon Etkilerinin Araştırılması

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Purification and characterization glutathione S-transferase enzyme from quail (Coturnix, coturnix japonica) heart and investigation the effect of some metal ions on enzyme activity

Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Effects of cadmium acetate contaminated drinking water on vital organs: A histopathological and biochemical study

Koyun Dalak Dokusundan Saflaştırılan Glukoz-6-Fosfat Dehidrogenaz ve Glutatyon Redüktaz Enzimi Üzerine Diklofenak Sodyumun İn Vitro Etkileri

Ameliorative effects of astaxanthin against copper(II) ion–induced alteration of pentose phosphate pathway and antioxidant system enzymes in rats

Oxidative Stress and Apoptotic Markers in Goats Naturally Infected with Mycobacterium avium subsp. paratuberculosis