Antibacterial and Antioxidant Activities of Ocimum basilicum L. Against Mastitis Pathogens

Gülten Ökmen; Neslihan Balpınar

doi:10.17776/csj.409675

EN TR

Antibacterial and Antioxidant Activities of Ocimum basilicum L. Against Mastitis Pathogens

Abstract

The most common bacterial agents cause inflammation in the mammary gland (mastitis), which disease cause to less yields in cows. These bacteria are Staphylococcus aureus and Coagulase-negative Staphylococci (CNS). Therefore, these bacteria were selected in this study. The purpose of this study is to research the antibacterial and antioxidant potential of the various extracts of Ocimum basilicum L. The mastitis agents used in the study are 7 bacteria in total; 2 of them are Staphylococcus aureus and the other 5 bacteria are CNS. The antibacterial activities studies were carried out using Kirby-Bauer disc diffusion technique. The other antibacterial activity test performed within the scope of this study was minimum inhibitory concentration (MIC) test. Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. This study showed that the largest inhibition zone was obtained from the methanol extract of O. basilicum. Result of this study is 9 mm. The lowest MIC value was recorded as 3250 μg/mL. It was found that O. basilicum used in the study had antibacterial activity against mastitis pathogens, and high antioxidant activity could be obtained from the water extracts (72%). Consequently, O. basilicum is a good candidate in developing new antibacterial and antioxidant agents.

Keywords

Antibacterial activity,Antioxidant activity,Mastitis,Ocimum basilicum,Ocimum basilicum

Mastitis Patojenlerine Karşı Ocimum basilicum L.’ un Antibakteriyel ve Antioksidan Aktiviteleri

Öz

En yaygın bakteriyel ajanlar meme bezlerinde iltihaplanmaya (mastitis) neden olmakta, ki bu hastalık ineklerde düşük verime neden olur. Bu bakteriler Staphylococcus aureus ve koagülaz-negatif Staphylococci (KNS)’dir. Bu çalışmanın amacı, Ocimum basilicum L.’nin çeşitli özütlerinin antibakteriyel ve antioksidan potansiyellerinin araştırılmasıdır. Çalışmada kullanılan mastitis etkenleri toplam 7 bakteri olup, bunlardan 2 tanesi Staphylococcus aureus, diğer 5 bakteri ise KNS’dir. Antibakteriyel aktivite çalışmaları Kirby-Bauer disk difüzyon metodu kullanılarak gerçekleştirilmiştir. Bu çalışma kapsamında yapılan diğer bir antibakteriyel aktivite testi, minimum inhibitör konsantrasyon (MİK) testidir. Antioksidan aktivite çalışmaları 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürme yöntemi ile belirlenmiştir. Çalışma en geniş inhibisyon zonunun, O. basilicum’un metanol özütünden sağlandığını göstermiştir. Bu çalışmanın sonucu 9 mm’dir. En düşük MİK değeri 3250 µg/mL olarak kayıt edilmiştir. Çalışmada kullanılan O. basilicum, mastitis patojenlerine karşı antibakteriyel aktiviteye sahiptir ve yüksek antioksidan aktivite sulu özütlerden elde edilmiştir (%72). Sonuç olarak, yeni antibakteriyel ve antioksidan ajanların geliştirilmesinde, O. basilicum’ un iyi bir aday olduğunu ortaya koymaktadır.

Anahtar Kelimeler

Antibakteriyel aktivite,Antioksidan aktivite,Mastitis,Ocimum basilicum

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Gülten Ökmen ^*
0000-0003-3207-6715
Türkiye

Neslihan Balpınar
0000-0002-4469-8629
Türkiye

Publication Date

September 30, 2018

Submission Date

March 26, 2018

Acceptance Date

September 24, 2018

Published in Issue

Year 2018 Volume: 39 Number: 3

DOI

https://doi.org/10.17776/csj.409675

IZ

https://izlik.org/JA27SR69PA

Cite

RIS / Bibtex

APA

Ökmen, G., & Balpınar, N. (2018). Antibacterial and Antioxidant Activities of Ocimum basilicum L. Against Mastitis Pathogens. Cumhuriyet Science Journal, 39(3), 573-580. https://doi.org/10.17776/csj.409675

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