Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes

Tülay Aşkın Çelik; Özlem Sultan Aslantürk

doi:10.17776/csj.405629

TR EN

Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. Sulu Ekstrelerinin in vitro İnsan Periferal Kan Lenfositleri Üzerindeki Sitotoksik ve Genotoksik Etkileri

Öz

Bu çalışmanın amacı Rosmarinus officinalis, Lavandula stoechas L. ve Tilia cordata Mill'den elde edilen sulu ekstrelerin (infüzyon ve dekoksiyon), insan periferal kan lenfositleri üzerindeki in vitro sitotoksik ve genotoksik etkisinin araştırılmasıdır.

Bu amaçla, 3 kadından alınan lenfositler, R. officinalis (1.5 ve 3 mg / ml), L. stoechas (0,4 ve 1,2 mg/ ml) ve T. cordata (1 mg / ml ve 3 mg / ml) sulu ekstreleri ile 48 saat süreyle muamele edilmiştir. Denemelerde PBS negatif kontrol olarak, Mitomisin C (MMC) (0,25 μg / ml) ise pozitif kontrol olarak kullanılmıştır. Ekstrelerin lenfositlerdeki sitotoksik etkisi, mitotik indeks hesaplanarak belirlenmiştir. Ayrıca, lenfositlerideki kromozom aberasyonları ve mikronukleus oluşumları da belirlenmiştir.

Deneyde kullanılan sulu ekstreler, kontrol gruplarına kıyasla konsantrasyon artışına bağlı olarak, periferal lenfosit hücrelerinde bölünmeyi önemli ölçüde azaltmıştır (p <0,01). Yine, metafazdaki hücrelerin sayısı da azalmıştır. Bu nedenle, periferal lenfositlerde kromozom incelemesi yapılamamıştır. Bununla birlikte, sulu ekstre muameleleri periferal lenfositlerde kontrol gruplarına kıyasla mikronukleus oluşumuna neden olmıuştur (p <0,01).

L. stoechas ve T. cordata'nın 3 mg / ml dekoksiyon ekstreleri, 48 saatlik muameleden sonra lenfositler üzerinde en yüksek sitotoksik etkiyi göstermiştir. En yüksek genotoksik etki ise 1.2 mg / ml L. stoechas dekoksiyon ekstresi ile muameleden sonra ortaya çıkmıştır.

Anahtar Kelimeler

Sitotoksik etki,genotoksik etki,insan periferal kan lenfositleri,Lavandula stoechas,Rosmarinus officinalis,Tilia cordata

Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes

Abstract

The aim of this study was to investigation of in vitro cytotoxic and genotoxic effects of infusion and decoction aqueous extracts obtained from Rosmarinus officinalis, Lavandula stoechas L. and Tilia cordata Mill. on human peripheral blood lymphocytes.

For this purpose, lymphocytes taken from 3 females, were treated with aqueous extracts of R. officinalis (1.5 and 3 mg/ml), L. stoechas (0,4 and 1,2 mg/ml) and T. cordata (1 mg/ml and 3 mg/ml) for 48 hours. PBS was used as negative control and Mitomycin C (MMC) (0,25 μg/ml) was used as positive control in experiments. Cytotoxic effect of extracts on lymphocytes was determined by calculating mitotic index. Also, chromosome aberrations micronucleus formations were determined.

All extracts considerably decreased the cell division in lymphocytes depending on the increased concentration in comparison with control groups (p<0,01). Also cells in metaphase decreased. Thus, no chromosomal aberration was observed in lymphocytes. However, extract treatments induced the formation of micronucleus in lymphocytes when compared to control groups (p<0,01). 3 mg/ml decoction extracts of L. stoechas and T. cordata showed the highest cytotoxic effect on lymphocytes, and the highest genotoxic effect appeared after 48 h treatment with 1.2 mg/ml decoction extract of L. stoechas.

Keywords

Cytotoxic effect,genotoxic effect,human peripheral lymphocytes,Lavandula stoechas,Rosmarinus officinalis,Tilia cordata

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Tülay Aşkın Çelik ^*

Özlem Sultan Aslantürk

Publication Date

March 16, 2018

Submission Date

July 7, 2017

Acceptance Date

November 30, 2017

Published in Issue

Year 2018 Volume: 39 Number: 1

DOI

https://doi.org/10.17776/csj.405629

IZ

https://izlik.org/JA44CS93WR

Cite

RIS / Bibtex

APA

Aşkın Çelik, T., & Aslantürk, Ö. S. (2018). Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes. Cumhuriyet Science Journal, 39(1), 127-143. https://doi.org/10.17776/csj.405629

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