Synthesis, Characterization and In Vitro Antioxidant Activities of New Benzimidazole Ruthenium- (II) Complexes

Serap Şahin Bölükbaşı; Neslihan Şahin

doi:10.17776/csj.349326

EN TR

Synthesis, Characterization and In Vitro Antioxidant Activities of New Benzimidazole Ruthenium- (II) Complexes

Abstract

In this study; it was first intended to synthesize 1-substituted benzimidazole ligands containing a nitrogen-bound bulky or functional group and then preparing Ru- (II) -N coordinated metal complexes of these ligands. At last stage of study it has been investigated that the antioxidant activities of synthesized metal-complexes. Inert atmosphere-vacuum system has been used for the synthesis of all complexes. Their structure were characterized by spectroscopic techniques. The in vitro antioxidant activities of metal complexes were investigated based on the scavenging of hydroxyl, superoxide and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. In addition, total antioxidant activity of each metal-complex was also determined. It was determined that total antioxidant activities of metal-complexes increased by the order of 3, 4 and 2. Because of decreasing IC₅₀values indicates that increasing antioxidant activity, DPPH radical scavenging activity of complex 2, which has lower IC₅₀value, was greater than complex 4. IC₅₀values have not seen for complex 3 for DPPH. It was observed that hydroxyl radical scavenging activities of complexes increased 4, 3 and 2, while superoxide radical scavenging activities of complexes increased by the order of 3, 2 and 4, respectively. As a result; newly synthesized Ru (II) -azole complexes have been found to have significant antioxidant activity against DPPH, hydroxyl and superoxide radicals.

Keywords

Benzimidazole,Ruthenium,DPPH,Hydroxyl Radical,Superoxide Radical

Yeni Benzimidazol Rutenyum-(II) Komplekslerinin Sentezi, Karakterizasyonu ve İn Vitro Antioksidan Aktiviteleri

Öz

Bu çalışmada; ilk olarak azota bağlı hacimli veya fonksiyonel grup içeren 1-sübstitüye benzimidazol ligantlarının sentezlenmesi ve daha sonra sentezlenen bu ligantların Ru-(II)-N-koordine metal komplekslerinin hazırlanması amaçlanmıştır. Son aşamada ise sentezlenen metal-komplekslerin antioksidan aktiviteleri çalışılmıştır. Bileşeklerin sentezlenmesinde inert atmosfer-vakum sistemi kullanılmıştır. Tüm komplekslerin yapıları spektroskopik tekniklerle aydınlatılmıştır. Sentezlenen metal komplekslerin in vitro antioksidan aktiviteleri; hidroksil, süperoksit ve DPPH radikallerini süpürme özellikleri ile araştırılmıştır. Ayrıca her bir metal-kompleksin toplam antioksidan aktivitesi de saptanmıştır. Metal-komplekslerin toplam antioksidan aktivitelerinin 3, 4 ve 2 sıralamasıyla arttığı saptanmıştır. Azalan IC₅₀değeri artan antioksidan aktiviteye işaret ettiğinden, daha düşük IC₅₀değerine sahip olan 2 numaralı bileşiğin DPPH radikali süpürme aktivitesinin 4 numaralı bileşikten daha yüksek olduğu bulunmuştur. DPPH radikali süpürme aktivitesinde, 3 numaralı bileşik için IC₅₀değeri gözlenmemiştir. Bileşiklerin hidroksil radikali süpürme aktivitelerinin 4, 3 ve 2 sıralamasıyla artarken, süperoksit radikali süpürme aktivitelerinin ise; 3, 2 ve 4 sıralamasıyla arttığı bulunmuştur. Sonuç olarak; yeni sentezlenen Ru(II)-azol komplekslerinin DPPH, hidroksil ve süperoksit radikallerine karşı önemli antioksidan aktiviteye sahip oldukları bulunmuştur.

Anahtar Kelimeler

Benzimidazol,Rutenyum,DPPH,Hidroksil Radikali,Süperoksit Radikali

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Serap Şahin Bölükbaşı

Neslihan Şahin

Publication Date

December 8, 2017

Submission Date

May 12, 2017

Acceptance Date

August 28, 2017

Published in Issue

Year 2017 Volume: 38 Number: 4

DOI

https://doi.org/10.17776/csj.349326

IZ

https://izlik.org/JA75CL37CG

Cite

RIS / Bibtex

APA

Şahin Bölükbaşı, S., & Şahin, N. (2017). Synthesis, Characterization and In Vitro Antioxidant Activities of New Benzimidazole Ruthenium- (II) Complexes. Cumhuriyet Science Journal, 38(4), 738-747. https://doi.org/10.17776/csj.349326