The Investigation of the Effects of Chlorpyrifos and 2,4-Dichlorophenoxyacetic Acid Application on Bovine Liver Catalase Activity
Abstract
In
this study, it was investigated whether different concentrations of
organophosphate insecticide chlorpyrifos (CPF) and systemic herbicide
2,4-dichlorophenoxyacetic acid (2,4-D)
on bovine liver catalase (CAT) activity cause any inhibitions or
activations. For this purpose, 25, 50, 100, 250 and 500 ppm concentrations of
CPF and 2,4-D were used. Following the applications of all tested
concentrations of the both pesticides, the CAT activity elevated. Under the
exposure of 25, 50, 100, 250 and 500 ppm concentrations, % CAT activity
increases were calculated as 10.0; 6.2; 4.6; 6.9 and 6.0 in CPF apllications,
while these increases were calculated as 13.1; 10.3; 17.0; 24.4 and 18.8 in
2,4-D applications, respectively. The present research indicated the elevations
in CAT activity with 2,4-D were higher compared to CPF. This means that 2,4-D
may have increased hydrogen peroxide production more than CPF.
Keywords
References
- [1]. Israel B., Common Insecticide May Harm Boys’ Brains More Than Girls. Scientific American 2012; August 21.
- [2]. Loomis D., Carcinogenicity of lindane, DDT, and 2,4-dichlorophenoxyacetic acid, The Lancet Oncology 2015; 16(8): 891-892.
- [3]. The National Institute for Occupational Safety and Health (NIOSH), The Effects of Workplace Hazards on Male Reproductive Health 2014; Accessed date: November 2017.
- [4]. Banerjee B.D., Seth V., Ahmed R.S., Pesticide-induced oxidative stress: perspectives and trends, Rev. Environ. Health, 2001; 16: 1–40.
- [5]. Attia, A.A., ElMazoudy, R.H., Nahla S. El-Shenawy, N.S., Antioxidant role of propolis extract against oxidative damage of testicular tissue induced by insecticide chlorpyrifos in rats, Pesticide Biochemistry and Physiology, 2012; 103: 87–93.
- [6]. Halliwell B., Gutteridge J. M. C., Free Radicals In Biology And Medicine. Third Edition, Oxford University Press., New York, 1999.
- [7]. Chelikani P., Fita I., Loewen P.C., Diversity of structures and properties among catalases, Cell Mol Life Sci. 2004; 61 (2): 192–208.
- [8]. Gaetani G., Ferraris A., Rolfo M., Mangerini R., Arena S., Kirkman H., Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes. Blood 1996; 87 (4): 1595–9.
Details
Primary Language
English
Subjects
-
Journal Section
Research Article
Authors
Hasan Karadağ
ADIYAMAN ÜNİVERSİTESİ
Türkiye
Publication Date
September 30, 2018
Submission Date
November 1, 2017
Acceptance Date
March 20, 2018
Published in Issue
Year 2018 Volume: 39 Number: 3