Effect of Blueberry and Raspberry on Activin-AB and Oxidative Stress Levels in Mice Given Azoxymethane and Dextran Sulfate Sodium

Müge Mavioğlu Kaya

doi:10.17776/csj.1856795

Effect of Blueberry and Raspberry on Activin-AB and Oxidative Stress Levels in Mice Given Azoxymethane and Dextran Sulfate Sodium

Abstract

There are insufficient clear studies on activin-AB (act-AB) as one of the isomers of activins that plays role in development of cancer. In this study aimed to investigate the effect of azoxymethane (AOM) and dextran sulfate sodium (DSS) as chemical agents on the act-AB level of colon tissue with oxidative stress and tumor necrosis factor-α (TNF-α) levels. A comparison of the effects of blueberries and blackberries was also planned. It was formed six equal groups of mice. Only intraperitoneal (ip) physiological serum was administered to control group (CNT). The second group (AOMD) received ip AOM/DSS combination. Third and fourth groups received oral aqueous raspberry (Rubus idaeus L.) and blueberry (Vaccinium myrtillus L.) extracts (RAE and VAE) in drinking water, respectively. Others received VAE and RAE plus AOM/DSS. While enzyme-linked immunosorbent assay was used for act-AB and TNF-α measurements, spectrophotometric methods were used for analyses of malondialdehyde (MDA) and reduced glutathion (GSH). AOMD act-AB levels were lower than other groups but significantly higher in VAE (p<0.05). MDA levels of AOMD were significantly higher than CNT, RAE and VAE while MDA levels of AOMD+VAE were lower than AOMD. Plasma GSH levels were higher in VAE compared to AOMD (p<0.01). TNF-α levels were lower in VAE (p<0.01) and AOMD+VAE groups. As a result, it was determined that VAE may normalize act-AB level in AOMD group. VAE was also found to be more effective than RAE for anti-inflammatory response associated with oxidative stress and TNF-α level during AOM and DSS application.

Keywords

Supporting Institution

Scientific Research Project Coordinator of Kafkas University

Project Number

KAUBAP 2019-FM-41

Ethical Statement

This study was approved by the Ethics Committee of Kafkas University Animal Experiments Local Ethics Committee (Ethical approval code: KAU-HADYEK 2019/024).

Thanks

The author would like to thank for support of the Kafkas University Scientific Research Council (KAUBAP Project number: 2019-FM-41). The author also thanks Prof. Dr. Özkan Özden for his guidance in selecting the methodology.

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Details

Primary Language

English

Subjects

Cell Development, Proliferation and Death

Journal Section

Research Article

Authors

Müge Mavioğlu Kaya ^*
0000-0003-1276-3745
Türkiye

Publication Date

April 29, 2026

Submission Date

January 6, 2026

Acceptance Date

April 13, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1856795

IZ

https://izlik.org/JA88RC33FC

Cite

RIS / Bibtex

APA

Mavioğlu Kaya, M. (2026). Effect of Blueberry and Raspberry on Activin-AB and Oxidative Stress Levels in Mice Given Azoxymethane and Dextran Sulfate Sodium. Cumhuriyet Science Journal, 47(2), 243-250. https://doi.org/10.17776/csj.1856795