Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC

Ünal Savsar; Bilal Yılmaz

doi:10.17776/csj.1840832

Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC

Abstract

The aim of this study was to develop and validate a rapid, simple and reliable high-performance liquid chromatography (HPLC) method for the accurate determination of hydroxymethylfurfural (HMF) in commercially available honey-flavored syrups. Analyses were performed using a reverse-phase HPLC system equipped with a 250 × 4.6 mm, 5 µm C₁₈ column and a UV detector set at 284 nm. The mobile phase consisted of methanol and acetonitrile (10:90, v/v), delivered at a flow rate of 1.0 mL min^-1. Under these conditions, HMF was quantified in less than 6 minutes, demonstrating the method’s efficiency and suitability for routine analysis. Method validation followed International Council for Harmonisation guidelines. The calibration curve showed linearity across 0.10-100 µg mL^-1 with a correlation coefficient above 0.99. Precision values for both intra-day and inter-day measurements were below 2.37%, while accuracy showed relative error values under 0.87%. Recovery tests, conducted using the standard addition method on three honey-flavored syrups (GrinTuss, Biakaf, Bisolnatur), confirmed the method’s robustness. Overall, the developed HPLC procedure provides a straightforward, sensitive and highly reproducible approach for determining HMF levels, supporting quality control and safety assessment of honey-flavored pharmaceutical syrups. This ensures its applicability for routine monitoring in diverse quality control settings today

Keywords

Supporting Institution

The research reported in this study was funded by the Atatürk University Scientific Research Projects Coordination Unit under project number TYL-2024-14871.

Project Number

TYL-2024-14871

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Details

Primary Language

English

Subjects

Separation Science, Analytical Chemistry (Other)

Journal Section

Research Article

Authors

Ünal Savsar
0009-0008-1972-3360
Türkiye

Bilal Yılmaz ^*
0000-0002-8574-7570
Türkiye

Publication Date

April 29, 2026

Submission Date

December 15, 2025

Acceptance Date

April 12, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1840832

IZ

https://izlik.org/JA63TH24WH

Cite

RIS / Bibtex

APA

Savsar, Ü., & Yılmaz, B. (2026). Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. Cumhuriyet Science Journal, 47(2), 343-349. https://doi.org/10.17776/csj.1840832

AMA

1.Savsar Ü, Yılmaz B. Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. CSJ. 2026;47(2):343-349. doi:10.17776/csj.1840832

Chicago

Savsar, Ünal, and Bilal Yılmaz. 2026. “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”. Cumhuriyet Science Journal 47 (2): 343-49. https://doi.org/10.17776/csj.1840832.

EndNote

Savsar Ü, Yılmaz B (April 1, 2026) Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. Cumhuriyet Science Journal 47 2 343–349.

IEEE

[1]Ü. Savsar and B. Yılmaz, “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”, CSJ, vol. 47, no. 2, pp. 343–349, Apr. 2026, doi: 10.17776/csj.1840832.

ISNAD

Savsar, Ünal - Yılmaz, Bilal. “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”. Cumhuriyet Science Journal 47/2 (April 1, 2026): 343-349. https://doi.org/10.17776/csj.1840832.

JAMA

1.Savsar Ü, Yılmaz B. Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. CSJ. 2026;47:343–349.

MLA

Savsar, Ünal, and Bilal Yılmaz. “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”. Cumhuriyet Science Journal, vol. 47, no. 2, Apr. 2026, pp. 343-9, doi:10.17776/csj.1840832.

Vancouver

1.Ünal Savsar, Bilal Yılmaz. Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. CSJ. 2026 Apr. 1;47(2):343-9. doi:10.17776/csj.1840832