Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells

Arzuhan Çetindağ; Sümeyye Nur Özbek; Levent Hacısüleyman

doi:10.17776/csj.1816408

Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells

Abstract

Oxidative stress plays a critical role in the pathogenesis of neurodegenerative diseases and glioma progression, with glial cells being central to maintaining redox homeostasis. Clonidine, an α2-adrenergic and imidazoline receptor agonist, has demonstrated neuroprotective and antioxidant effects in various experimental models. This study aimed to evaluate the protective effects of clonidine against hydrogen peroxide (H₂O₂)-induced oxidative damage in C6 glial cells. C6 glioma cells were pretreated with clonidine (0.3–4.8 µM) for 1 hour, followed by exposure to 0.5 mM H₂O₂ for 24 hours, and cell viability was assessed using the XTT assay, while total antioxidant status (TAS), total oxidant status (TOS), and superoxide dismutase (SOD) levels were measured using commercial kits and ELISA. H₂O₂ exposure significantly reduced cell viability and antioxidant parameters while increasing oxidative markers; however, clonidine treatment significantly improved cell viability, elevated TAS and SOD levels, and decreased TOS levels compared to the H₂O₂ group, demonstrating its dose-dependent protective effect against oxidative stress. These findings suggest that clonidine mitigates oxidative damage in C6 glial cells, potentially through antioxidant, anti-inflammatory, and imidazoline receptor-mediated mechanisms, and may have therapeutic relevance in conditions characterized by glial oxidative stress, including neurodegenerative diseases and glioma.

Keywords

Supporting Institution

TÜBİTAK

Project Number

1919B012108393

Ethical Statement

Not applicable. This study did not involve human participants or animal experiments

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Arzuhan Çetindağ ^*
0000-0002-5420-3546
Türkiye

Sümeyye Nur Özbek
0000-0002-8832-7774
Türkiye

Levent Hacısüleyman
0000-0002-4374-9025
Türkiye

Publication Date

April 29, 2026

Submission Date

November 5, 2025

Acceptance Date

March 3, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1816408

IZ

https://izlik.org/JA96CJ87AL

Cite

RIS / Bibtex

APA

Çetindağ, A., Özbek, S. N., & Hacısüleyman, L. (2026). Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells. Cumhuriyet Science Journal, 47(2), 216-222. https://doi.org/10.17776/csj.1816408

AMA

1.Çetindağ A, Özbek SN, Hacısüleyman L. Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells. CSJ. 2026;47(2):216-222. doi:10.17776/csj.1816408

Chicago

Çetindağ, Arzuhan, Sümeyye Nur Özbek, and Levent Hacısüleyman. 2026. “Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells”. Cumhuriyet Science Journal 47 (2): 216-22. https://doi.org/10.17776/csj.1816408.

EndNote

Çetindağ A, Özbek SN, Hacısüleyman L (April 1, 2026) Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells. Cumhuriyet Science Journal 47 2 216–222.

IEEE

[1]A. Çetindağ, S. N. Özbek, and L. Hacısüleyman, “Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells”, CSJ, vol. 47, no. 2, pp. 216–222, Apr. 2026, doi: 10.17776/csj.1816408.

ISNAD

Çetindağ, Arzuhan - Özbek, Sümeyye Nur - Hacısüleyman, Levent. “Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells”. Cumhuriyet Science Journal 47/2 (April 1, 2026): 216-222. https://doi.org/10.17776/csj.1816408.

JAMA

1.Çetindağ A, Özbek SN, Hacısüleyman L. Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells. CSJ. 2026;47:216–222.

MLA

Çetindağ, Arzuhan, et al. “Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells”. Cumhuriyet Science Journal, vol. 47, no. 2, Apr. 2026, pp. 216-22, doi:10.17776/csj.1816408.

Vancouver

1.Arzuhan Çetindağ, Sümeyye Nur Özbek, Levent Hacısüleyman. Clonidine Attenuates Oxidative Stress Induced by H₂O₂ in C6 Glial Cells. CSJ. 2026 Apr. 1;47(2):216-22. doi:10.17776/csj.1816408