Antiproliferative Effects of Galium Aparine in SNU-1 Gastric Cancer Cells and Its Association with Growth Signaling Pathways

Mesut Parlak; Emre Gedikli; Zıad Joha

doi:10.17776/csj.1750248

Antiproliferative Effects of Galium Aparine in SNU-1 Gastric Cancer Cells and Its Association with Growth Signaling Pathways

Abstract

Galium aparine (G. aparine) is a plant with a long history of ethnopharmacological use and has been investigated for various biological activities, including potential anticancer effects. The present study aimed to investigate the in vitro effects of G. aparine extract on SNU-1 human gastric carcinoma cells and to examine its potential role in the inhibition of cell proliferation. Our experimental setup involved exposing SNU-1 cells to G. aparine (12.5–200 µg/mL) for 24 hours. Cell viability was then evaluated using the XTT assay, and ELISA measured the levels of growth signal factors mTOR, HER-2, EGFR, and ERK-1/2. The XTT assay revealed a significant reduction in cell viability (p<0.001). Furthermore, ELISA measurements showed that G. aparine treatment notably decreased the levels of crucial growth signal factors, including mTOR, HER-2, EGFR, and ERK-1/2 (p<0.05 to p<0.01). These findings suggest that the antiproliferative activity of Galium aparine against gastric cancer cells may be associated with the modulation of key growth signaling pathways involved in cancer cell survival and proliferation. This highlights the potential of G. aparine as a natural agent that could be further investigated as a complementary or alternative approach for gastric cancer research.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Mesut Parlak
0000-0001-9692-8396
Türkiye

Emre Gedikli
0000-0001-5593-2073
Türkiye

Zıad Joha ^*
0000-0001-8520-3760
Türkiye

Publication Date

April 29, 2026

Submission Date

July 28, 2025

Acceptance Date

February 22, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1750248

IZ

https://izlik.org/JA28BW26SY

Cite

RIS / Bibtex

APA

Parlak, M., Gedikli, E., & Joha, Z. (2026). Antiproliferative Effects of Galium Aparine in SNU-1 Gastric Cancer Cells and Its Association with Growth Signaling Pathways. Cumhuriyet Science Journal, 47(2), 202-207. https://doi.org/10.17776/csj.1750248