Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line

Fatih Yulak; İrem Cinaz

doi:10.17776/csj.1742233

Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line

Abstract

This study aimed to explore the neuroprotective properties of everolimus and its association with oxidative stress. The HT-22 hippocampal neuronal cell line was utilized as the in vitro model. To assess the protective effects of everolimus, four experimental groups were designed. The control group received no treatment. The pentylenetetrazol (PTZ) group was exposed to 60 mM PTZ for 1 hour. In the everolimus group, cells were treated with increasing concentrations of everolimus (0.1, 0.5, 1, 5, and 10 nM) for 2 hours. In the combination group (everolimus + PTZ), cells were pre-treated with everolimus at the same concentrations for 1 hour, followed by 60 mM PTZ exposure for an additional hour. Cell viability was assessed via the XTT assay, while oxidative stress was evaluated by measuring total antioxidant status (TAS) and total oxidant status (TOS) using commercial assay kits. Treatment with everolimus significantly enhanced cell viability at concentrations of 5 and 10 nM (p < 0.01). Additionally, TAS levels were markedly elevated, and TOS levels were significantly reduced in the PTZ + everolimus (10 nM) group compared to the PTZ-only group (p < 0.01). These findings suggest that everolimus confers neuroprotection by enhancing the antioxidant defense system and mitigating PTZ-induced cytotoxicity

Keywords

Supporting Institution

TÜBİTAK

Project Number

1919B012310182

Ethical Statement

This study does not require ethical declaration.

Thanks

We express our gratitude to TÜBİTAK and the Research Center of Cumhuriyet University Faculty of Medicine (CÜTFAM) for providing the necessary resources to carry out this research.

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Details

Primary Language

English

Subjects

Cell Development, Proliferation and Death

Journal Section

Research Article

Authors

Fatih Yulak ^*
0000-0003-3708-6752
Türkiye

İrem Cinaz
0009-0003-3907-8665
Türkiye

Publication Date

April 29, 2026

Submission Date

July 14, 2025

Acceptance Date

March 9, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1742233

IZ

https://izlik.org/JA98AC45CT

Cite

RIS / Bibtex

APA

Yulak, F., & Cinaz, İ. (2026). Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line. Cumhuriyet Science Journal, 47(2), 223-229. https://doi.org/10.17776/csj.1742233

AMA

1.Yulak F, Cinaz İ. Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line. CSJ. 2026;47(2):223-229. doi:10.17776/csj.1742233

Chicago

Yulak, Fatih, and İrem Cinaz. 2026. “Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line”. Cumhuriyet Science Journal 47 (2): 223-29. https://doi.org/10.17776/csj.1742233.

EndNote

Yulak F, Cinaz İ (April 1, 2026) Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line. Cumhuriyet Science Journal 47 2 223–229.

IEEE

[1]F. Yulak and İ. Cinaz, “Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line”, CSJ, vol. 47, no. 2, pp. 223–229, Apr. 2026, doi: 10.17776/csj.1742233.

ISNAD

Yulak, Fatih - Cinaz, İrem. “Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line”. Cumhuriyet Science Journal 47/2 (April 1, 2026): 223-229. https://doi.org/10.17776/csj.1742233.

JAMA

1.Yulak F, Cinaz İ. Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line. CSJ. 2026;47:223–229.

MLA

Yulak, Fatih, and İrem Cinaz. “Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line”. Cumhuriyet Science Journal, vol. 47, no. 2, Apr. 2026, pp. 223-9, doi:10.17776/csj.1742233.

Vancouver

1.Fatih Yulak, İrem Cinaz. Exploring the Protective Role of Everolimus in Preventing Pentylenetetrazole-Triggered Injury in Hippocampal Neurons Using the HT-22 Cell Line. CSJ. 2026 Apr. 1;47(2):223-9. doi:10.17776/csj.1742233