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Understanding of Protein Adhesion to PLGA Through Molecular Docking Analysis

Year 2025, Volume: 46 Issue: 4, 689 - 697, 30.12.2025

Didem Mimiroğlu , Sema Zabcı

https://doi.org/10.17776/csj.1706951

Abstract

In tissue engineering, cellular adhesion is a multistep process which occurs between the cell and the specific molecules and proteins that are adsorbed from biological fluids to the material surface. Therefore, it is essential to understand the protein adsorption level and which protein can bind efficiently to the surface of the fabricated material. Although many studies in literature showing quantification of protein adsorption levels by chemical methods and some instrumental techniques, there is a notable gap in the literature regarding the application of molecular docking analysis to identify which proteins can bind effectively to material surfaces, together with the specific interactions and orientations involved. Towards this goal, the present study aimed to evaluate the interactions between FDA approved biomaterial poly(lactic-co-glycolic acid) (PLGA) surface and specific proteins which are commonly found in cell culture medium for in vitro studies, and integrins which are associated with cellular adhesion. The results showed that PLGA exhibited strong binding affinity to both BSA and integrin β2, while less stable bindings were performed by hemoglobin and 2-macroglobulin proteins. These results indicate that how molecular recognition via docking process is important for fabricating biomaterials in tissue engineering applications.

Keywords

Molecular docking PLGA Cellular adhesion Serum proteins

Ethical Statement

Not applicable

Project Number

The Scientific and Technological Research Council of Turkey (grant no: 217M952).

Thanks

The authors would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK, Grant No: 217M952) for its partial financial support.

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  • Başka bir talebiniz var mı? Bu referansların genel bir bibliyografyasını oluşturmamı veya belirli bir bölüme göre (örneğin protein yapısı vs.) gruplandırmamı ister misiniz?
There are 39 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Article
Authors

Didem Mimiroğlu 0000-0003-2838-9719

Sema Zabcı 0000-0002-8886-0077

Project Number The Scientific and Technological Research Council of Turkey (grant no: 217M952).
Submission Date May 26, 2025
Acceptance Date November 18, 2025
Publication Date December 30, 2025
Published in Issue Year 2025 Volume: 46 Issue: 4

Cite

APA Mimiroğlu, D., & Zabcı, S. (2025). Understanding of Protein Adhesion to PLGA Through Molecular Docking Analysis. Cumhuriyet Science Journal, 46(4), 689-697. https://doi.org/10.17776/csj.1706951

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