Synthesis and In Vitro Evaluation of Novel Thiazole-Hydrazone Derivatives with Antioxidant and Cytotoxic Potential

Ebru Nur Ay; Tugba Ercetin; Ulviye Acar Çevik

doi:10.17776/csj.1700142

Synthesis and In Vitro Evaluation of Novel Thiazole-Hydrazone Derivatives with Antioxidant and Cytotoxic Potential

Abstract

A new series of thiazole–hydrazone derivatives (2a–2l) was synthesized and evaluated for their in vitro antioxidant and cytotoxic properties. The structures of the synthesized compounds were confirmed by ¹H NMR, ¹³C NMR, and HRMS analyses. Antioxidant activity was assessed using the DPPH radical scavenging assay, while cytotoxicity was evaluated using the MTT assay in human colorectal cancer (HCT116) and non-small cell lung cancer (H460) cell lines. In the DPPH assay, compounds 2c and 2h exhibited the notable radical scavenging activity, with IC₅₀ values of 31.73 ± 0.004 μM and 31.13 ± 0.008 μM, respectively, comparable to the reference compound gallic acid (IC₅₀ = 29.48 ± 0.014 μM). Cytotoxicity screening performed at 100 μM for 24 h indicated that most derivatives did not significantly reduce cell viability in HCT116 cells. In contrast, a partial decrease in cell viability was observed in H460 cells for the selected compounds, with compound 2l showing a moderate effect (IC₅₀ = 82.00 ± 3.67 μM). The results indicate that the biological activity of these thiazole–hydrazone derivatives is strongly influenced by substitution patterns. While the compounds exhibited limited antiproliferative effects under the tested conditions, certain derivatives demonstrated notable antioxidant activity. These findings suggest that this scaffold may serve as a useful starting point for further structural optimization toward multifunctional bioactive molecules

Keywords

References

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Details

Primary Language

English

Subjects

Cancer Biology , Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Ebru Nur Ay ^*
0000-0001-7121-4362
Türkiye

Tugba Ercetin
0000-0001-7774-7266
Kuzey Kıbrıs Türk Cumhuriyeti

Ulviye Acar Çevik
0000-0003-1879-1034
Türkiye

Publication Date

April 29, 2026

Submission Date

May 15, 2025

Acceptance Date

April 20, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1700142

IZ

https://izlik.org/JA98AW82XJ

Cite

RIS / Bibtex

APA

Ay, E. N., Ercetin, T., & Acar Çevik, U. (2026). Synthesis and In Vitro Evaluation of Novel Thiazole-Hydrazone Derivatives with Antioxidant and Cytotoxic Potential. Cumhuriyet Science Journal, 47(2), 276-285. https://doi.org/10.17776/csj.1700142