Metabolic Adaptations: Comparison of Amino Acid Profiles of SK-MEL-30 Melanoma Cells and CCD-1135Sk Fibroblasts

Nebiye Pelin Türker

doi:10.17776/csj.1698280

Metabolic Adaptations: Comparison of Amino Acid Profiles of SK-MEL-30 Melanoma Cells and CCD-1135Sk Fibroblasts

Abstract

This study provides original data on metabolic reprogramming of tumor cells by comparing amino acid profiles between malignant melanoma cells and healthy human dermal fibroblast cells. Quantitative amino acid analyses performed on SKMEL-30 (melanoma) and CCD-1135Sk (normal fibroblast) cells using LC-MS/MS technology showed that the total amino acid load in melanoma cells was approximately 80% higher than in normal fibroblasts (7595.94 ng/mL vs. 4225.35 ng/mL). Significant increases were detected in amino acids such as glutamate, valine, leucine, isoleucine, serine, alanine, threonine, and glycine. These increases support processes such as rapid proliferation of tumor cells, meeting energy needs, maintaining redox balance, and stress response. In addition, the dramatic increase in metabolites such as taurine and GABA in melanoma cells suggests that these cells gain a microsurvival advantage. The findings reveal that melanoma-specific amino acid metabolism has significant potential for biomarker development and targeted metabolic treatment approaches.

Keywords

Ethical Statement

Not applicable

Thanks

I appreciate the Trakya University Technology Research Development Application and Research Center (TUTAGEM) for providing all of the lab equipment.

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Details

Primary Language

English

Subjects

Cell Metabolism , Cancer Biology , Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Nebiye Pelin Türker ^*
0000-0001-6060-3557
Türkiye

Publication Date

September 30, 2025

Submission Date

May 13, 2025

Acceptance Date

September 18, 2025

Published in Issue

Year 2025 Volume: 46 Number: 3

DOI

https://doi.org/10.17776/csj.1698280

IZ

https://izlik.org/JA63ST29SP

Cite

RIS / Bibtex

APA

Türker, N. P. (2025). Metabolic Adaptations: Comparison of Amino Acid Profiles of SK-MEL-30 Melanoma Cells and CCD-1135Sk Fibroblasts. Cumhuriyet Science Journal, 46(3), 506-511. https://doi.org/10.17776/csj.1698280