Investigating the Efficacy of Cannabidiol Against Arsenic-Induced Liver Cell Damage

Serkan Şahin; Vugar Ali Türksoy; Serap Yalcin

doi:10.17776/csj.1616411

TR EN

Arsenik neden olduğu karaciğer hücre hasarında karşı kannabidiolün etkinliğinin değerlendirlmesi

Öz

Arsenik (As) maruziyeti, zararlı etkileri bilinen bir durumdur ve etkili karşı önlemler arayışını teşvik etmektedir. Bu çalışmada, kannabidiolün (CBD) arsenik toksisitesine karşı karaciğer hücre kültürlerinde (THLE-2) koruyucu etkileri araştırılmıştır. As için IC50 değerleri belirlenmiş ve 2D hücre hatlarında 10 µM, 3D hücre hatlarında ise 15 µM bulunmuştur. CBD'nin koruyucu etkinliğini değerlendirmek amacıyla, IC50 konsantrasyonunda arsenik ile birlikte 5 µM CBD uygulanmıştır. Oksidatif stres belirteçleri olan GSH, MDA, MPO ve CAT seviyelerinin yanı sıra, inflamatuar sitokinler TNF-α, IL-1β ve IL-6, ELISA kitleri kullanılarak ölçülmüştür. As alımını değerlendirmek için ICP-MS analizi yapılmıştır. Sonuçlar, CBD'nin hücre proliferasyonunu önemli ölçüde artırdığını ve arsenik maruziyetiyle indüklenen oksidatif stres ve inflamatuar yanıtları tersine çevirdiğini göstermiştir. Ayrıca, CBD'nin arsenik alımını hücrelere etkili bir şekilde azalttığı tespit edilmiştir. Bu bulgular, CBD'nin arsenik kaynaklı toksisiteye karşı koruyucu rolünü güçlü bir şekilde desteklemekte ve arsenikin zararlı etkilerini hafifletmede terapötik ajan olarak potansiyelini vurgulamaktadır.

Anahtar Kelimeler

Supporting Institution

Yozgat Bozok University Scientific Research Projects Unit

Project Number

6608-TF/21-475

Ethical Statement

Ethical approval is not required as this is a cell culture study.

Thanks

This study is supported by Yozgat Bozok University Scientific Research Projects Unit (Project number: 6608-TF/21-475).

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Details

Primary Language

English

Subjects

Pharmaceutical Toxicology , Basic Pharmacology

Journal Section

Research Article

Authors

Serkan Şahin ^*
0000-0001-7000-6342
Türkiye

Vugar Ali Türksoy
0000-0002-3545-3945
Türkiye

Serap Yalcin
0000-0002-9584-266X
Türkiye

Publication Date

March 25, 2025

Submission Date

January 9, 2025

Acceptance Date

March 17, 2025

Published in Issue

Year 1970 Volume: 46 Number: 1

DOI

https://doi.org/10.17776/csj.1616411

IZ

https://izlik.org/JA62RP57ZR

Cite

RIS / Bibtex

APA

Şahin, S., Türksoy, V. A., & Yalcin, S. (2025). Investigating the Efficacy of Cannabidiol Against Arsenic-Induced Liver Cell Damage. Cumhuriyet Science Journal, 46(1), 55-61. https://doi.org/10.17776/csj.1616411

Investigating the Efficacy of Cannabidiol Against Arsenic-Induced Liver Cell Damage

Arsenik neden olduğu karaciğer hücre hasarında karşı kannabidiolün etkinliğinin değerlendirlmesi

Öz

Anahtar Kelimeler