Cytotoxic Activity, Anti-migration and in silico Study of o-Coumaric acid on H1975 Non-small Cell Lung Cancer Cells

Doğukan Mutlu

doi:10.17776/csj.1592097

TR EN

o-Kumarik Asidin H1975 Küçük Hücreli Olmayan Akciğer Kanseri Hücreleri Üzerinde Sitotoksik Aktivitesi, Anti-migrasyon ve in-Silico Çalışması

Öz

Akciğer kanseri, dünya genelinde en yaygın kanser türü ve kansere bağlı ölümlerin önde gelen nedenidir. Fenolik asitler, özellikle antikanser özellikleri nedeniyle çeşitli biyolojik aktiviteleriyle dikkat çeken bir grup bileşiktir. Bu çalışma, o-kumarik asidin (OCA) sitotoksik aktivitesini, anti-migrasyon etkilerini ve moleküler yerleştirme analizini değerlendirmektedir. H1975 hücrelerindeki OCA'nın in vitro sitotoksisitesi, MTT testi ve AO/EB boyaması kullanılarak değerlendirilirken, hücre göçü üzerindeki etkisi in vitro yara iyileşme testi (scratch assay) ile analiz edilmiştir. OCA, H1975 hücrelerine karşı sitotoksik aktivite göstermiş, IC50 değeri 8.107 mM olarak belirlenmiş ve hücre göçünü %38 oranında inhibe etmiştir. Ayrıca, in silico moleküler yerleştirme analizi, epidermal büyüme faktörü reseptörü (EGFR) ile etkileşimini araştırmak için gerçekleştirilmiştir. Sonuçlar, OCA'nın EGFR'ye güçlü bir bağlanma afinitesi sergilediğini ortaya koymuştur. Bu bulgular, OCA'nın küçük hücreli dışı akciğer kanserine karşı terapötik bir ajan olarak potansiyele sahip olduğunu ve endüstriyel ve klinik uygulamalarına yönelik daha fazla araştırma yapılması gerektiğini göstermektedir.

Anahtar Kelimeler

Cytotoxic Activity, Anti-migration and in silico Study of o-Coumaric acid on H1975 Non-small Cell Lung Cancer Cells

Abstract

Lung cancer represents the most common malignancy and remains the primary cause of cancer-related deaths worldwide. Phenolic acids, including o-coumaric acid (OCA), have attracted considerable attention due to their diverse biological activities, particularly their anticancer properties. This study evaluates the cytotoxic activity of OCA, along with its anti-migration effects and molecular docking analysis. The in vitro cytotoxicity of OCA on H1975 cells was assessed using the MTT assay and Acridine orange/ethidium bromide (AO/EB) staining, while its impact on cell migration was analyzed through an in vitro scratch assay. OCA demonstrated cytotoxic activity against H1975 cells, with an IC50 value of 8.107 mM, and inhibited cell migration by 38%. Additionally, in silico molecular docking was performed to investigate its interaction with the epidermal growth factor receptor (EGFR). Although OCA exhibited notable binding interactions with EGFR, including hydrogen bonding and pi-alkyl interactions, its binding affinity (−5.9 kcal/mol) was lower compared to Gefitinib (−8.5 kcal/mol), a known EGFR inhibitor. These findings suggest that while OCA holds potential as a therapeutic agent against non-small cell lung cancer, its efficacy may be enhanced through structural modifications, including the synthesis of derivatives, warranting further research into its industrial and clinical applications

Keywords

References

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Details

Primary Language

English

Subjects

Molecular Docking , Cell Development, Proliferation and Death , Cancer Biology

Journal Section

Research Article

Authors

Doğukan Mutlu ^*
0000-0003-3259-5822
Türkiye

Publication Date

March 25, 2025

Submission Date

November 27, 2024

Acceptance Date

February 13, 2025

Published in Issue

Year 2025 Volume: 46 Number: 1

DOI

https://doi.org/10.17776/csj.1592097

IZ

https://izlik.org/JA54AL23UK

Cite

RIS / Bibtex

APA

Mutlu, D. (2025). Cytotoxic Activity, Anti-migration and in silico Study of o-Coumaric acid on H1975 Non-small Cell Lung Cancer Cells. Cumhuriyet Science Journal, 46(1), 35-40. https://doi.org/10.17776/csj.1592097

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