Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells

Zıad Joha; Sude Nur Göksu

doi:10.17776/csj.1535242

EN

Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells

Abstract

Previous studies have demonstrated the anticancer properties of certain calcium channel blockers when administered as sole agents. This exploration aimed to explore the potential anti-proliferative activity of nifedipine on AGS gastric cancer cells and to determine the contribution of oxidative stress in mediating this response. To assess the anti-proliferative impact of nifedipine, AGS cell viability was calculated employing the XTT assay across a concentration range of 25, 50, 100, 200, and 500 µg/mL. Concurrently, TAS and TOS kits were employed to evaluate the drug's influence on oxidative stress levels. Nifedipine exhibited a concentration-dependent cytotoxic effect on AGS cells, with a statistically significant reduction in cell viability. The IC50 value for AGS cells after 24 hours was determined to be 98.49 µg/mL. At this concentration, a substantial augmentation in TOS and a concurrent diminution in TAS levels were observed relative to the control group. These observations imply that nifedipine-induced oxidative stress is one of the mechanisms underlying its cytotoxic action against gastric cancer cells. Our results highlight the potential therapeutic utility of nifedipine in this cancer type.

Keywords

Supporting Institution

TÜBİTAK

Project Number

1919B012221382

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Zıad Joha ^*
0000-0001-8520-3760
Türkiye

Sude Nur Göksu
0009-0007-9021-2021
Türkiye

Publication Date

December 30, 2024

Submission Date

August 18, 2024

Acceptance Date

December 16, 2024

Published in Issue

Year 2024 Volume: 45 Number: 4

DOI

https://doi.org/10.17776/csj.1535242

IZ

https://izlik.org/JA85HL56CT

Cite

RIS / Bibtex

APA

Joha, Z., & Göksu, S. N. (2024). Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells. Cumhuriyet Science Journal, 45(4), 683-687. https://doi.org/10.17776/csj.1535242

AMA

1.Joha Z, Göksu SN. Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells. CSJ. 2024;45(4):683-687. doi:10.17776/csj.1535242

Chicago

Joha, Zıad, and Sude Nur Göksu. 2024. “Oxidative Stress Mediates Anti-Proliferative Effects of Nifedipine on AGS Cells”. Cumhuriyet Science Journal 45 (4): 683-87. https://doi.org/10.17776/csj.1535242.

EndNote

Joha Z, Göksu SN (December 1, 2024) Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells. Cumhuriyet Science Journal 45 4 683–687.

IEEE

[1]Z. Joha and S. N. Göksu, “Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells”, CSJ, vol. 45, no. 4, pp. 683–687, Dec. 2024, doi: 10.17776/csj.1535242.

ISNAD

Joha, Zıad - Göksu, Sude Nur. “Oxidative Stress Mediates Anti-Proliferative Effects of Nifedipine on AGS Cells”. Cumhuriyet Science Journal 45/4 (December 1, 2024): 683-687. https://doi.org/10.17776/csj.1535242.

JAMA

1.Joha Z, Göksu SN. Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells. CSJ. 2024;45:683–687.

MLA

Joha, Zıad, and Sude Nur Göksu. “Oxidative Stress Mediates Anti-Proliferative Effects of Nifedipine on AGS Cells”. Cumhuriyet Science Journal, vol. 45, no. 4, Dec. 2024, pp. 683-7, doi:10.17776/csj.1535242.

Vancouver

1.Zıad Joha, Sude Nur Göksu. Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells. CSJ. 2024 Dec. 1;45(4):683-7. doi:10.17776/csj.1535242