Characterization of Local Okra Seed (Abelmoschus Esculentus) Extract and Evaluation of Bioactivity

Bilgenur Çelebi; Sevil Ozer; Sezen Canım Ateş

doi:10.17776/csj.1519528

EN

Characterization of Local Okra Seed (Abelmoschus Esculentus) Extract and Evaluation of Bioactivity

Abstract

A. esculentus (okra) plant is an economically important medicinal plant grown in tropical and subtropical parts of the world. The fruit and seeds of the A. esculentus (okra) plant; which are rich in oil and protein. The in vitro cytotoxic, bactericidal, and antioxidant activities of extracts obtained from different parts of okra (leaves, fruits, and seeds) were studied widely in the medical industry. This study evaluated the biocompatible and antioxidant potential of an optimized ethanolic extract of A. esculentus seeds. Commercially obtained A. esculentus seeds were first extracted and characterized by UV-Vis and FT-IR spectrophotometry. In vitro, antioxidant activity of okra seed extracts was evaluated using 1,1 -diphenyl -2picrylhydrazyl (DPPH) with different concentrations (0.5-5 mg/mL). Results show that the antioxidant activity increased in direct proportion to the increase in the concentration of the extract. In vitro, the cytotoxic effect of the extracts on C6 Glioma cancer cell line and L929 mouse fibroblast cell line was studied using 3-(4, 5-dimethylthiazolyl -2,5-diphenyl-tetrazolium bromide (MTT) assay with the concentrations between 50-1000 µg/mL. MTT results showed effective cytotoxicity on cancer cell lines with increasing extract concentration (IC50 value for C6 was 273.4 µg/ml, while the IC50 value for L929 was 431.45 µg/ml). The study indicates that the extract isolated from A. esculentus seeds shows that all concentrations have a substantial amount of anticancer and antioxidant activity. In conclusion, A. esculentus seeds could be considered innovative products and be proposed for alternative end-uses in pharmaceutical industries with antimicrobial and bioactive properties.

Keywords

References

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Details

Primary Language

English

Subjects

Cellular Interactions , Genotoxicity and Cytotoxicity , Cytogenetic

Journal Section

Research Article

Authors

Bilgenur Çelebi
0009-0006-5737-1840
Türkiye

Sevil Ozer ^*
0000-0002-0186-763X
Türkiye

Sezen Canım Ateş
0000-0003-2196-7053
Türkiye

Publication Date

December 30, 2024

Submission Date

July 20, 2024

Acceptance Date

December 16, 2024

Published in Issue

Year 2024 Volume: 45 Number: 4

DOI

https://doi.org/10.17776/csj.1519528

IZ

https://izlik.org/JA58XR28KP

Cite

RIS / Bibtex

APA

Çelebi, B., Ozer, S., & Canım Ateş, S. (2024). Characterization of Local Okra Seed (Abelmoschus Esculentus) Extract and Evaluation of Bioactivity. Cumhuriyet Science Journal, 45(4), 707-712. https://doi.org/10.17776/csj.1519528

Cited By

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Pharmacological Research - Modern Chinese Medicine

https://doi.org/10.1016/j.prmcm.2025.100678

Öz

Characterization of Local Okra Seed (Abelmoschus Esculentus) Extract and Evaluation of Bioactivity

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Evaluation of the Cardio-Protective Effect of Saponin-Rich Fraction of Dioscorea bulbifera in Mitigating Doxorubicin-Induced Cardiotoxicity in Rats: A Microanatomical Study