Isolation and Characterization of Heat Shock Protein 70-Ipek 1 from Toxoplasma gondii

Kübra Açıkalın Coşkun; Lütfi Tutar

doi:10.17776/csj.1501849

EN

Isolation and Characterization of Heat Shock Protein 70-Ipek 1 from Toxoplasma gondii

Abstract

Toxoplasma gondii is a common intracellular parasite that causes the toxoplasmosis. Heat shock proteins (Hsps) have a critical role in pathogenesis of toxoplasmosis. Hsps are highly conserved proteins in evolution among living organisms. This protein family responsible for a wide range of biological processes such as protein folding, protein translocation, protein aggregation. In the present study, Hsp70, a member of the Hsp family, was isolated from T. gondii and its sequence and motifs were determined by PCR, cloning, sequencing and homology modelling analysis. ATP hydrolysis, luciferase folding, and luciferase aggregation experiments were performed for determination of its chaperone activity while the stability and secondary structure of the Hsp70 were discovered by using biophysical experiments (FTIR, florescence and quenching experiment). In addition, in silico analysis were used to determine the physicochemical characteristics of Hsp70. The results revealed that Hsp70 protein obtained from T. gondii (Hsp70-IPEK1) is similar to Hsp70s from other organisms. Also, the chaperone activity, stability and secondary structure of Hsp70-IPEK1 were determined. Hsp70-IPEK1 together with other chaperones in the presence of nucleotide were dramatically increased protein folding and aggregation. According to these results, it is thought that Hsp70 has a potential to contribute many research areas such as pharmaceutical analysis

Keywords

References

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Details

Primary Language

English

Subjects

Analytical Biochemistry , Protein Trafficking , Medical Biochemistry - Proteins, Peptides and Proteomics

Journal Section

Research Article

Authors

Kübra Açıkalın Coşkun ^*
0000-0002-0047-8368
Türkiye

Lütfi Tutar
0000-0002-6260-3136
Türkiye

Publication Date

September 30, 2024

Submission Date

June 15, 2024

Acceptance Date

September 15, 2024

Published in Issue

Year 2024 Volume: 45 Number: 3

DOI

https://doi.org/10.17776/csj.1501849

IZ

https://izlik.org/JA28AG95CN

Cite

RIS / Bibtex

APA

Açıkalın Coşkun, K., & Tutar, L. (2024). Isolation and Characterization of Heat Shock Protein 70-Ipek 1 from Toxoplasma gondii. Cumhuriyet Science Journal, 45(3), 496-502. https://doi.org/10.17776/csj.1501849

Öz

Isolation and Characterization of Heat Shock Protein 70-Ipek 1 from Toxoplasma gondii

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite