Influence of Selected Natural Antioxidants on Iron-Induced Enzymatic Alterations Related to Oxidative Stress

Melike Karaman; Emine Toraman

doi:10.17776/csj.1425012

EN

Influence of Selected Natural Antioxidants on Iron-Induced Enzymatic Alterations Related to Oxidative Stress

Abstract

Iron is required in various biological processes of the cell, but excess iron causes oxidative stress. Oxidative stress can be prevented by antioxidants with free radical scavenging properties. Tannic acid and gallic acid are phenolic compounds with antioxidant properties found naturally in plants. In this study, the effects of gallic acid and tannic acid on iron-induced oxidative stress parameters were investigated in a fruit fly model. Effect of the compounds against iron-induced oxidative stress were evaluated by determining spectrophotometrically superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and acetylcholinesterase (AChE) enzyme activities, and levels of reduced glutathione (GSH) and malondialdehyde (MDA) in larvae (n: 10) and adults (n: 20) of wild type Oregon R strain of Drosophila melanogaster. Iron treatment decreased enzyme activities and GSH levels, but increased MDA levels. Co-treatment of these compounds with iron ameliorated iron-induced changes, especially in larvae. On the other hand, iron-induced decrease in AChE activity was increased in adults by treatment of these compounds with iron. The results showed that natural phenolic compounds have the potential to ameliorate iron-induced changes in oxidative stress parameters.

Keywords

References

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Details

Primary Language

English

Subjects

Enzymes

Journal Section

Research Article

Authors

Melike Karaman ^*
0000-0002-0973-2561
Türkiye

Emine Toraman
0000-0001-7732-6189
Türkiye

Publication Date

June 30, 2024

Submission Date

January 24, 2024

Acceptance Date

June 13, 2024

Published in Issue

Year 2024 Volume: 45 Number: 2

DOI

https://doi.org/10.17776/csj.1425012

IZ

https://izlik.org/JA34WM52GW

Cite

RIS / Bibtex

APA

Karaman, M., & Toraman, E. (2024). Influence of Selected Natural Antioxidants on Iron-Induced Enzymatic Alterations Related to Oxidative Stress. Cumhuriyet Science Journal, 45(2), 256-262. https://doi.org/10.17776/csj.1425012

Öz

Influence of Selected Natural Antioxidants on Iron-Induced Enzymatic Alterations Related to Oxidative Stress

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite