Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities

İlkay Açıkgöz Erkaya; Dilek Yalçın; Belgin Erdem

doi:10.17776/csj.1408553

EN

Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities

Abstract

In this study, both dry and fresh biomass extracts of Klebsormidium subtile were used for the synthesis of silver nanoparticles (AgNPs). The UV-visible spectrum showed an absorption peak at 430 nm, indicating the presence of AgNPs through surface plasmon resonance. FT-IR analysis identified bioactive functional groups, such as amines, which acted as stabilizing agents for the nanoparticles. SEM imaging revealed well-dispersed, spherical AgNPs ranging from 5 to 25 nm and 40 to 60 nm in size, accumulating on cell surfaces. EDS analysis confirmed the presence of elemental silver. The antimicrobial activity of AgNPs derived from both fresh and dry K. subtile extracts was similar, though AgNPs from the dry extract were more effective against Staphylococcus aureus, with inhibition zones of 15.8, 16.2, and 15.2 mm at 1 mM, 2 mM, and 3 mM concentrations, respectively. AgNPs also showed strong activity against Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans, but were less effective against Bacillus cereus and Aeromonas hydrophila. These findings suggest that K. subtile-derived AgNPs have significant antimicrobial potential, particularly against S. aureus and C. albicans, and may be useful in biomedical applications, particularly for treating biofilm-related infections.

Keywords

Supporting Institution

Kırşehir Ahievran University

Project Number

MMF.A4.21.009

References

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Details

Primary Language

English

Subjects

Cell Metabolism , Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

İlkay Açıkgöz Erkaya
0000-0003-1730-4951
Türkiye

Dilek Yalçın ^*
0000-0003-2127-8186
Türkiye

Belgin Erdem
0000-0001-9108-5561
Türkiye

Publication Date

September 30, 2024

Submission Date

December 22, 2023

Acceptance Date

September 15, 2024

Published in Issue

Year 2024 Volume: 45 Number: 3

DOI

https://doi.org/10.17776/csj.1408553

IZ

https://izlik.org/JA89NB82JZ

Cite

RIS / Bibtex

APA

Açıkgöz Erkaya, İ., Yalçın, D., & Erdem, B. (2024). Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities. Cumhuriyet Science Journal, 45(3), 454-464. https://doi.org/10.17776/csj.1408553

Öz

Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite