The Anti-proliferative Effect of Caffeic Acid and Dactolisib on Human Cervical Carcinoma HeLa Cell Line

Zeynep Büşra Bolat

doi:10.17776/csj.1394831

EN

The Anti-proliferative Effect of Caffeic Acid and Dactolisib on Human Cervical Carcinoma HeLa Cell Line

Abstract

Cervical carcinoma is a common gynecological cancer with high mortality rate among women worldwide. Caffeic acid exerts an antiproliferative effect against cervical carcinoma. Dactolisib is a dual PI3K and mTOR inhibitor that has a therapeutic potential for cervical carcinoma. This study aimed to reveal the anti-proliferative effect of combination treatment of caffeic acid and Dactolisib on cervical carcinoma HeLa cell line. Cytotoxicity of caffeic acid and Dactolisib on HeLa cell line was assessed by MTS assay. Colony formation of HeLa cells treated with caffeic acid and Dactolisib was determined by staining colonies with crystal violet and visualizing under light microscope. Dactolisib decreased cell proliferation of HeLa cells in time and dose dependent manner. 5 μM caffeic acid did not show any significant change in cell viability of HeLa cells. Combination treatment of 5 μM caffeic acid and 0.5 μM Dactolisib showed decrease in cell viability of HeLa cells when compared to Dactolisib treated cells. Combination of caffeic acid and Dactolisib decreased colony diameter of HeLa cells significantly when compared to control group. Caffeic acid and Dactolisib shows anti-proliferative effect on human cervical carcinoma HeLa cell line, so further studies should be performed to reveal the mechanism of action.

Keywords

Supporting Institution

Funding: This study was funded partially by Istanbul Sabahattin Zaim University BAP grant number: BAP1000-102.

Project Number

BAP1000-102

Ethical Statement

Ethics Committee Approval: Not required.

Thanks

Acknowledgements: The human cervical carcinoma HeLa cell lines was a kind gift of Prof. Aysegul Dogan at Yeditepe University.

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Zeynep Büşra Bolat ^*
0000-0002-9216-6336
Türkiye

Publication Date

March 28, 2024

Submission Date

November 23, 2023

Acceptance Date

February 27, 2024

Published in Issue

Year 2024 Volume: 45 Number: 1

DOI

https://doi.org/10.17776/csj.1394831

IZ

https://izlik.org/JA25PK96SU

Cite

RIS / Bibtex

APA

Bolat, Z. B. (2024). The Anti-proliferative Effect of Caffeic Acid and Dactolisib on Human Cervical Carcinoma HeLa Cell Line. Cumhuriyet Science Journal, 45(1), 15-19. https://doi.org/10.17776/csj.1394831

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Drug repurposing identifies novel Wee1 kinase inhibitors for triple negative breast cancer therapeutics

European Journal of Medicinal Chemistry

https://doi.org/10.1016/j.ejmech.2025.118340

Öz

The Anti-proliferative Effect of Caffeic Acid and Dactolisib on Human Cervical Carcinoma HeLa Cell Line

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Exploring the mechanisms of action of polyphenols in the treatment of cervical cancer

Drug repurposing identifies novel Wee1 kinase inhibitors for triple negative breast cancer therapeutics