Cytotoxicity Analysis of the Effects of Heterobasidion Annosum Mycelia and Cisplatin on Colon Adenocarcinoma (CACO-2) Cell Line

Çiğdem Sevim; Sabri Ünal; Temel Kan Bakır; Mertcan Karadeniz; Ali Taghizadeh Tgzd

doi:10.17776/csj.1357215

EN

Cytotoxicity Analysis of the Effects of Heterobasidion Annosum Mycelia and Cisplatin on Colon Adenocarcinoma (CACO-2) Cell Line

Abstract

Colorectal cancer ranks as the third most prevalent form of cancer and stands as the second leading cause of mortality. Both environmental and genetic risk factors contribute to its manifestation. Presently, 5-fluoruracil/leucovorin (5-FU/LV) remains the recommended course for adjuvant therapy in addressing this condition. Conversely, mushrooms, celebrated for their biologically active constituents, including valuable enzymes, have emerged as a captivating subject in diverse medical disciplines, particularly within the realm of cancer therapy, due to their promising therapeutic properties. This specific investigation aimed to conduct in vitro cytotoxic experiments using extracts obtained from Heterobasidion annosum micelles cultivated in a liquid malt extract medium. The pulverized extracts were dissolved in Dulbecco's Modified Eagle Medium (DMEM) at varied concentrations ranging from 25ng/mL to 200ng/mL and subsequently administered to colon adenocarcinoma (Caco-2) cells. The cytotoxic effects of both the fungus and cisplatin, a well-known anticarcinogenic agent, were examined at intervals of 24, 48, and 72 hours. The findings indicated a significant inhibition of cancer cell development within this timeframe. Moreover, a noteworthy discovery emerged, revealing that cisplatin, known for its efficacy in various cancer studies, substantially diminished the viability of cancer cells after 72 hours in comparison to the control group.

Keywords

Thanks

We would like to thank Isparta University of Applied Sciences Faculty of Forestry faculty member Dr. Lecturer Ayşe Gülden ADAY KAYA for providing the Heterobasidion annosum isolate TR501 used in the study and contributing to our study.

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Çiğdem Sevim ^*
0000-0002-0575-3090
Türkiye

Sabri Ünal
0000-0002-3026-0597
Türkiye

Temel Kan Bakır
0000-0002-7447-1468
Türkiye

Mertcan Karadeniz
0000-0002-3627-9424
Türkiye

Ali Taghizadeh Tgzd
Türkiye

Publication Date

March 28, 2024

Submission Date

September 8, 2023

Acceptance Date

February 27, 2024

Published in Issue

Year 2024 Volume: 45 Number: 1

DOI

https://doi.org/10.17776/csj.1357215

IZ

https://izlik.org/JA95YP32DC

Cite

RIS / Bibtex

APA

Sevim, Ç., Ünal, S., Bakır, T. K., Karadeniz, M., & Tgzd, A. T. (2024). Cytotoxicity Analysis of the Effects of Heterobasidion Annosum Mycelia and Cisplatin on Colon Adenocarcinoma (CACO-2) Cell Line. Cumhuriyet Science Journal, 45(1), 105-110. https://doi.org/10.17776/csj.1357215

Öz

Cytotoxicity Analysis of the Effects of Heterobasidion Annosum Mycelia and Cisplatin on Colon Adenocarcinoma (CACO-2) Cell Line

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite