T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

Mustafa Ergül

doi:10.17776/csj.1238418

EN

T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

Abstract

Accumulating evidence reports that T-type calcium channels play crucial roles in tumor formation and development. However, the roles of inhibiting calcium channels in tumor cells with various inhibitors in tumor progression remain unclear. This study aimed to investigate the cytotoxic and apoptotic effects of NNC 55-0396, a T-type calcium channel inhibitor, against SNU-1 gastric cancer cells. Cytotoxic and apoptotic effects of NNC 55-0396 were evaluated by the XTT assay and flow cytometry. The results showed that NNC 55-0396 had concentration-dependent cytotoxicity in SNU-1 cells and its the half-maximal inhibitory concentration (IC50) value was calculated as 4.17 µM. The results of the Annexin V experiments also showed that this inhibitor significantly increased apoptosis in SNU-1 cells. In conclusion, these results demonstrated that NNC 55-0396 induces cytotoxic effects by increasing apoptosis in gastric cancer cells. However, further research is required for its use as a possible therapeutic agent in the treatment of gastric cancer.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Mustafa Ergül ^*
0000-0003-4303-2996
Türkiye

Publication Date

June 30, 2023

Submission Date

January 18, 2023

Acceptance Date

June 7, 2023

Published in Issue

Year 2023 Volume: 44 Number: 2

DOI

https://doi.org/10.17776/csj.1238418

IZ

https://izlik.org/JA37TK34AX

Cite

RIS / Bibtex

APA

Ergül, M. (2023). T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal, 44(2), 254-257. https://doi.org/10.17776/csj.1238418

Cited By

Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells

Cumhuriyet Science Journal

https://doi.org/10.17776/csj.1535242

T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

Abstract

Keywords

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Oxidative Stress Mediates Anti-proliferative Effects of Nifedipine on AGS Cells