Concomitant strain of Bacillus vallismortis BR2 and Escherichia coli Khodavandi-Alizadeh-2 for Biocatalytic Synthesis of Fatty Acid Methyl Ester from Waste Oil Feedstock

Michael Osho; Olayinka Mary Otolorin

doi:10.17776/csj.1206615

EN

Concomitant strain of Bacillus vallismortis BR2 and Escherichia coli Khodavandi-Alizadeh-2 for Biocatalytic Synthesis of Fatty Acid Methyl Ester from Waste Oil Feedstock

Abstract

Bacillus vallismortis BR2 and Escherichia coli Khodavandi-Alizadeh-2 lipases (E.C.3.1.1.3) were used to produce fatty acid methyl ester (FAME), a sustainable source of fuel. The lipase activity was measured using the titrimetric method after it was extracted from a solid fermented substrate in phosphate buffer. The use of Central Composite Design to optimize condition parameters was examined, while qualitative and quantitative assessments of FAME samples were performed using GC-MS with MSD in scan mode and selective ion monitoring. Lipase activity peaked at 24 h and then declined as the incubation time went on. The independent variables, such as pH, temperature, agitation, incubation time and enzyme quantity, all had an effect on biodiesel yield since they were all significant in the rate of biodiesel yield. FAME yield increased significantly after adding 1 to 2 mL of enzyme and a pH range of 4.57143 to 7.42857, but thereafter declined. The chromatograms indicated a peak of cis-10-Heptadecanoic acid methyl ester with concentrations of 39.95 mg/L and 58.95 mg/L in the FAME molecules. The viscosity (3.67 m3/s), specific gravity (0.813 g/cm3), flash point (102.70 °C), cetane number (55.52), and pour point (-24 °C) of the fuel were also measured. The synthesized biodiesel from the spent oil through the synergic enzymes were found to be a simple, effective, and sustainable fuel production process, as well as a potential means of eliminating pollution caused by haphazard waste cooking oil disposal.

Keywords

References

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Details

Primary Language

English

Subjects

Biomaterial

Journal Section

Research Article

Authors

Michael Osho ^*
0000-0003-1177-8363
Nigeria

Olayinka Mary Otolorin
0009-0000-1306-1839
Nigeria

Publication Date

June 30, 2023

Submission Date

November 18, 2022

Acceptance Date

June 20, 2023

Published in Issue

Year 2023 Volume: 44 Number: 2

DOI

https://doi.org/10.17776/csj.1206615

IZ

https://izlik.org/JA86KD69EM

Cite

RIS / Bibtex

APA

Osho, M., & Otolorin, O. M. (2023). Concomitant strain of Bacillus vallismortis BR2 and Escherichia coli Khodavandi-Alizadeh-2 for Biocatalytic Synthesis of Fatty Acid Methyl Ester from Waste Oil Feedstock. Cumhuriyet Science Journal, 44(2), 244-253. https://doi.org/10.17776/csj.1206615

Concomitant strain of Bacillus vallismortis BR2 and Escherichia coli Khodavandi-Alizadeh-2 for Biocatalytic Synthesis of Fatty Acid Methyl Ester from Waste Oil Feedstock

Öz

Anahtar Kelimeler

Concomitant strain of Bacillus vallismortis BR2 and Escherichia coli Khodavandi-Alizadeh-2 for Biocatalytic Synthesis of Fatty Acid Methyl Ester from Waste Oil Feedstock

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite