The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations

Deniz Şahin İnan; Zübeyde Akın Polat; Rasim Hamutoğlu

doi:10.17776/csj.1180547

EN

The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations

Abstract

This study aimed to associate the possible cytotoxic and apoptotic effects of Botox (Allergan) and Dysport (Ipsen) and immunolocalization of matrix metalloproteinase (MMP) proteins with HCN2 cortical neuron cell line. Accordingly, cytotoxic potentials of Botox and Dysport were determined on different concentrations. Then, the apoptosis rates of these cells were evaluated by TUNEL method. MMP2, MMP7, and MMP9 proteins were also visualized using immunofluorescence method. There was a significant difference in cytotoxicity between those treated with 3.2, 6.4, and 12.8IU compared with the control. The Dysport 12.8 IU group was statistically more toxic than Botox group at the same concentration. Therefore, the number of apoptotic cells increased from 0.1 IU in Botox and 0.01 IU in Dysport groups compared to the control. The number of apoptotic cells was significantly higher in Dysport group at 1.6, 3.2, 6.4, and 12.8 IU concentrations than in Botox group. It was determined that MMPs increased gradually at the concentrations where the number of apoptotic cells was highest compared to the control group. As a result, we consider that it may be necessary to deal with the dose adjustment in Botox and Dysport applications, together with detailed studies to be carried out in the future.

Keywords

Supporting Institution

CÜBAP

Project Number

T-756

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Deniz Şahin İnan ^*
0000-0002-0292-4448
Türkiye

Zübeyde Akın Polat
0000-0002-1130-4953
Türkiye

Rasim Hamutoğlu
0000-0002-2474-5336
Türkiye

Publication Date

March 26, 2023

Submission Date

September 26, 2022

Acceptance Date

March 14, 2023

Published in Issue

Year 2023 Volume: 44 Number: 1

DOI

https://doi.org/10.17776/csj.1180547

IZ

https://izlik.org/JA64RR87SP

Cite

RIS / Bibtex

APA

Şahin İnan, D., Akın Polat, Z., & Hamutoğlu, R. (2023). The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations. Cumhuriyet Science Journal, 44(1), 19-27. https://doi.org/10.17776/csj.1180547

Öz

The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations

Abstract

Keywords

Supporting Institution

Project Number

References

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Primary Language

Subjects

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Publication Date

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Acceptance Date

Published in Issue

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