The Effects of Lapatinib and Trastuzumab in a Rat Model of Endometriosis

Çağlar Yıldız; Zeki Özsoy; Turgut Kacan; Hatice Özer

doi:10.17776/csj.1168698

EN

The Effects of Lapatinib and Trastuzumab in a Rat Model of Endometriosis

Abstract

Trastuzumab and lapatinib are drugs belonging to tyrosine kinase inhibitors family that are used in cancer treatment to prevent cell proliferation. Trastuzumab is an inhibitor of human epidermal growth factor receptor–2 (HER2) tyrosine kinase, and lapatinib is an inhibitor of epidermal growth factor receptor (EGFR). Tyrosine kinase inhibitors have also been investigated for treatment of endometriosis. In the present study, we aimed to investigate the effects of lapatinib and trastuzumab on rat endometriosis model. Endometriosis was surgically induced by the autologous transplantation of endometrial tissue and formation of endometriosis was confirmed via secondary laparotomy in 32 rats. Initially, 4 mg/kg dose of trastuzumab was applied intraperitoneally, and two additional doses of 2 mg/kg were applied 7 days and 14 days after the initial dose. Lapatinib was administered as 100 mg/kg daily doses for 14 days. Rats were randomly divided into four groups and were subjected to lapatinib, trastuzumab, anastrozole (0.004 mg/day, p.o.) and normal saline (0.1 ml, i.p.) treatments for 14 days. Then, endometriosis foci were excised, and endometriosis scores were calculated in a semi-quantitative manner. Immunohistochemical (IHC) examinations were also performed using VEGF, CD117 and Bax antibodies. Both anastrozole and tyrosine kinase inhibitors lowered endometriosis scores. Significant decreases in ovarian follicle numbers were observed in lapatinib and anastrozole groups but not trastuzumab group. Lapatinib and trastuzumab decreased endometriotic foci through suppressing cell proliferation and promoting programmed cell death.

Keywords

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Çağlar Yıldız ^*
0000-0003-3150-3340
Türkiye

Zeki Özsoy
0000-0003-0749-4157
Türkiye

Turgut Kacan
0000-0002-2067-1560
Türkiye

Hatice Özer
0000-0001-6479-3626
Türkiye

Publication Date

December 27, 2022

Submission Date

August 30, 2022

Acceptance Date

November 24, 2022

Published in Issue

Year 2022 Volume: 43 Number: 4

DOI

https://doi.org/10.17776/csj.1168698

IZ

https://izlik.org/JA75LP78NG

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RIS / Bibtex

APA

Yıldız, Ç., Özsoy, Z., Kacan, T., & Özer, H. (2022). The Effects of Lapatinib and Trastuzumab in a Rat Model of Endometriosis. Cumhuriyet Science Journal, 43(4), 556-563. https://doi.org/10.17776/csj.1168698

Öz

Anahtar Kelimeler

The Effects of Lapatinib and Trastuzumab in a Rat Model of Endometriosis

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite