Evaluation of DNA Protective and Antimicrobial Properties of some Cladonia Species

Ahmet Ceylan; Gökçe Şeker Karatoprak; Zekiye Kocakaya; Mustafa Kocakaya

doi:10.17776/csj.1141849

EN

Evaluation of DNA Protective and Antimicrobial Properties of some Cladonia Species

Abstract

The present study evaluated the DNA protective properties and antimicrobial activities of the methanol extracts of nine Cladonia species, namely C. pocillum, C. subulata, C. pyxidata, C. coniocraea, C. foliacea, C. firma, C. furcata, C. fimbriata and C. rangiformis collected in Turkey. DNA protection properties efficiency of Cladonia extracts was evaluated using pBR322 plasmid DNA. In vitro antimicrobial activities of methanol extracts against two Gram-negative bacteria (Escherichia coli and Proteus mirabilis), three Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis) and two fungal strains (Candida glabrata and Candida albicans) were examined using the disc diffusion method and through the determination of minimal inhibitory concentrations (MIC). DNA protective studies, all Cladonia extracts protected pBR322 plasmid DNA against damage caused by the hydrogen peroxide (H2O2) with ultraviolet (UV). The results demonstrated that the inhibition zones in the disc diffusion method ranged from 6.5 to 19.0 mm. MIC results were ranged from 3.12 to 6.25 mg/mL. Cladonia extracts show a better antimicrobial effect against bacterial strains than fungal strains. The highest antimicrobial effect among lichen species was demonstrated by Cladonia pocillum. Our results demonstrated that tested Cladonia extracts had strong antibacterial and DNA protective effects. This is the first comprehensive study to evaluate the DNA protective properties activity of Cladonia extracts.

Keywords

Thanks

We would like to thank the research councils of Erciyes University and Bozok University for the financial support.

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ahmet Ceylan ^*
0000-0002-3087-066X
Türkiye

Gökçe Şeker Karatoprak
0000-0001-5829-6914
Türkiye

Zekiye Kocakaya
0000-0001-5248-0462
Türkiye

Mustafa Kocakaya
0000-0003-2306-8094
Türkiye

Publication Date

December 27, 2022

Submission Date

July 7, 2022

Acceptance Date

October 2, 2022

Published in Issue

Year 2022 Volume: 43 Number: 4

DOI

https://doi.org/10.17776/csj.1141849

IZ

https://izlik.org/JA68SD77DW

Cite

RIS / Bibtex

APA

Ceylan, A., Şeker Karatoprak, G., Kocakaya, Z., & Kocakaya, M. (2022). Evaluation of DNA Protective and Antimicrobial Properties of some Cladonia Species. Cumhuriyet Science Journal, 43(4), 550-555. https://doi.org/10.17776/csj.1141849

Evaluation of DNA Protective and Antimicrobial Properties of some Cladonia Species

Öz

Anahtar Kelimeler

Evaluation of DNA Protective and Antimicrobial Properties of some Cladonia Species

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite