Araştırma Makalesi
Cytotoxicity, Radical Scavenging, Antioxidant Properties and Chemical Composition of the Essential Oil of Satureja cilicica P.H. Davis from Turkey
Öz
Satureja cilicica P.H. Davis is an endemic species of Lamiaceae,
distributed in Turkey. In this study, the essential oil of S.
cilicica was obtained by hydrodistillation from the aerial
parts. The essential oil was analyzed by GC/MS and the main
constituents are identified. Radical scavenging capacities of oil
obtained from S. cilicica were determined by using DPPH and
ABTS radical scavenging assays. In addition, ferric-reducing
power of the essential oil was detected. Cytotoxic activity
of the essential oil was determined by MTT assay after 24 h
treatment of MCF-7. The major components of the essential
oil are p-cymene (17.68%), carvacrol (14.02%), γ-terpinene
(11.23 %) and thymol (8.76%). Radical scavenging capacities of
the essential oil were measured as 3.28±0.02 and 238.15±3.59
mg Trolox equivalent per milliliter of oil in term of DPPH and
ABTS radical scavenging, respectively. Ferric-reducing power
of essential oil was found 39.76±3.66 mg Trolox equivalent per
milliliter of essential oil. Growth inhibition effect of essential
oil against MCF-7 cancer cell is established. According to the
results, essential oil of S. cilicica exhibit low cytotoxic activity,
with IC50 values, with IC50 value of 268 μg/mL. The findings of
the analyzed species are compared with the results of previous
studies.
Anahtar Kelimeler
Kaynakça
- 1. Ćavara S, Maksimović M, Šolić ME, Jerković-Mujkić A, Bešta R. Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils. Food Chem 2008; 111: 648-53.
- 2. Hajhashemi V, Ghannadi A, Pezeshkian SK. Antinociceptive and anti-inflammatory effects of Satureja hortensis L.extracts and essential oil. J Ethnopharmacol 2002; 82: 83-7.
- 3. Kadereit JW. Flowering Plants. Dicotyledons: Lamiales. In: The Families and genera of vascular plants. Editor: Kubitzki K. Springer. Vol. 7. 2004, pp 239.
- 4. Öztekin M. Satureja L. In: A checklist of the Flora of Turkey (Vascular Plants). Editors: Güner A, Aslan S, Ekim T, Vural M, Babaç MT. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul. 2012, pp 581–582.
- 5. Ozkan G, Simsek B, Kuleasan H. Antioxidant activities of Satureja cilicica essential oil in butter and in vitro. J Food Eng 2007 79: 1391-6.
- 6. Momtaz S, Abdollahi M. A Systematic review of the biological activities of Satureja L. species. PhOL 2008; 2:34-54.
- 7. Sadeghi I, Yousefzadi M, Behmanesh M, Sharifi M, Moradi A. In vitro cytotoxic and antimicrobial activity of essential oil from Satureja intermedia. Iran Red Crescent Medical J 2013; 15:70-4.
- 8. Kundaković T, Stanojković T, Kolundžija B, Markovi, S, Šukilović B, Milenković M, Lakušić B. Cytotoxicity and antimicrobial activity of the essential oil from Satureja montana subsp. pisidica (Lamiceae). Nat Prod Commun 2014; 9: 569-72.
- 9. Schulz H, Özkan G, Baranska M, Krüger H, Özcan M. Characterisation of essential oil plants from Turkey by IR and Raman spectroscopy. Vib Spectrosc 2005; 39: 249-56.
- 10. Tümen G, Baser KHC, Kirimer N. The essential oil of Satureja cilicica P.H. Davis. J Essent Oil Res 1993; 5:547-8.
- 11. Davis PH. Satureja L. In: Flora of Turkey and the East Aegean Islands, Vol. 7. Editor: Davis PH. Edinburgh University Press, Edinburgh. 1982, pp. 314–323.
- 12. Başer KHC, Tümen G, Tabanca N, Demirci F. Composition and antibacterial activity of the essential oils from Satureja wiedemanniana (Lallem.) Velen. Z Naturforsch 2001; 56c: 731-8.
- 13. NIST (National Institute of Standards and Technology). <http://webbook.nist.gov/chemistry/name-ser.html> (accessed March 2017).
- 14. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995; 28: 25-30.
- 15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med 1999; 26: 1231-7.
- 16. Oyaizu M. Antioxidative activities of browning products of glucosamine ractionated by organic solvent and thin layer chromatography. Nippon Shokuhin Gakk 1988; 35: 771-5.
- 17. Runyoro D, Ngassapa O, Vagionas K, Aligiannis N, Graikou K, Chinou I. Chemical composition and antimicrobial activity of the essential oils of four Ocimum species growing in Tanzania. Food Chem 2010 119: 311-6.
- 18. Stajner D, Milic N, Mimica-Dukic N, Lazic B, Igic R. Antioxidant abilities of cultivated and wild species of garlic. Phytotherapy Res 1998; 12: 13-4.
- 19. Malencic D, Gasiç O, Popovic M, Boza P. Screening for antioxidant properties of Salvia reflexa Hornem. Phytother Res 2000; 14: 546-8.
- 20. Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994; 315: 161-9.
- 21. Ulukanli Z, Karaborklu S, Bozok F, Ates B, Erdogan S, Cenet M, Karaaslan MG. Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils. Chin J Nat Med 2014; 12: 901-10.
- 22. Miri R, Firuzi O, Peymani P, Nazarian Z, Shafiee A. Synthesis and Cytotoxicity Study of New Cyclopenta [b] quinoline-1,8- dione Derivatives. Iran J PharmRes 2011;10:489–96.
Türkiye’de Yetişen Satureja cilicica P.H. Davis Türünün Uçucu Yağının Sitotoksisitesi, Antioksidan Aktivitesi ve Kimyasal Kompozisyonu
Öz
Satureja cilicica P.H. Davis Lamiaceae familyasının Türkiye’de
yetişen endemik bir türüdür. Bu çalışmada, S. cilicica’nın uçucu
yağı bitkinin toprak üstü kısımlarından su distilasyonu yöntemi
ile elde edilmiştir. Uçucu yağ GC/MS ile analiz edilmiş ve ana
bileşenleri belirlenmiştir. S. cilicica’dan elde edilen yağın radikal
süpürme kapasiteleri DPPH radikali ve ABTS radikali süpürme
yöntemi ile belirlenmiştir. Ayrıca uçucu yağın ferrik indirgeme
gücü tespit edilmiştir. Uçucu yağın sitotoksik aktivitesi 24
saat MCF-7 ile muamele edildikten sonra MTT yöntemi
ile belirlenmiştir. p-Simen (%17.68), karvakrol %(14.02),
γ-terpinen (%11.23 ) ve timol (%8.76) uçucu yağın ana
bileşenleri olarak saptanmıştır. Uçucu yağın radikal süpürme
kapasiteleri sırasıyla DPPH ve ABTS radikal süpürme yöntemi
ile 3,28±0,02 ve 238,15±3,59 mg eşdeğer trolox/mL uçucu yağ
olarak ölçülmüştür. Uçucu yağın ferrik indirgeme gücü, 39,76
± 3,66 mg eşdeğer trolox/mL uçucu yağ olarak bulunmuştur.
Uçucu yağın MCF-7 kanser hücresine karşı büyüme inhibisyon
etkisi araştırılmıştır. Sonuç olarak, S. cilicica’nın uçucu yağının
268 μg/mL IC50 değeri ile düşük sitotoksik aktiviteye sahip
olduğu görülmüştür. Analiz edilen türden elde edilen veriler
önceki çalışmalar ile karşılaştırılmıştır.
Anahtar Kelimeler
Kaynakça
- 1. Ćavara S, Maksimović M, Šolić ME, Jerković-Mujkić A, Bešta R. Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils. Food Chem 2008; 111: 648-53.
- 2. Hajhashemi V, Ghannadi A, Pezeshkian SK. Antinociceptive and anti-inflammatory effects of Satureja hortensis L.extracts and essential oil. J Ethnopharmacol 2002; 82: 83-7.
- 3. Kadereit JW. Flowering Plants. Dicotyledons: Lamiales. In: The Families and genera of vascular plants. Editor: Kubitzki K. Springer. Vol. 7. 2004, pp 239.
- 4. Öztekin M. Satureja L. In: A checklist of the Flora of Turkey (Vascular Plants). Editors: Güner A, Aslan S, Ekim T, Vural M, Babaç MT. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul. 2012, pp 581–582.
- 5. Ozkan G, Simsek B, Kuleasan H. Antioxidant activities of Satureja cilicica essential oil in butter and in vitro. J Food Eng 2007 79: 1391-6.
- 6. Momtaz S, Abdollahi M. A Systematic review of the biological activities of Satureja L. species. PhOL 2008; 2:34-54.
- 7. Sadeghi I, Yousefzadi M, Behmanesh M, Sharifi M, Moradi A. In vitro cytotoxic and antimicrobial activity of essential oil from Satureja intermedia. Iran Red Crescent Medical J 2013; 15:70-4.
- 8. Kundaković T, Stanojković T, Kolundžija B, Markovi, S, Šukilović B, Milenković M, Lakušić B. Cytotoxicity and antimicrobial activity of the essential oil from Satureja montana subsp. pisidica (Lamiceae). Nat Prod Commun 2014; 9: 569-72.
- 9. Schulz H, Özkan G, Baranska M, Krüger H, Özcan M. Characterisation of essential oil plants from Turkey by IR and Raman spectroscopy. Vib Spectrosc 2005; 39: 249-56.
- 10. Tümen G, Baser KHC, Kirimer N. The essential oil of Satureja cilicica P.H. Davis. J Essent Oil Res 1993; 5:547-8.
- 11. Davis PH. Satureja L. In: Flora of Turkey and the East Aegean Islands, Vol. 7. Editor: Davis PH. Edinburgh University Press, Edinburgh. 1982, pp. 314–323.
- 12. Başer KHC, Tümen G, Tabanca N, Demirci F. Composition and antibacterial activity of the essential oils from Satureja wiedemanniana (Lallem.) Velen. Z Naturforsch 2001; 56c: 731-8.
- 13. NIST (National Institute of Standards and Technology). <http://webbook.nist.gov/chemistry/name-ser.html> (accessed March 2017).
- 14. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995; 28: 25-30.
- 15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med 1999; 26: 1231-7.
- 16. Oyaizu M. Antioxidative activities of browning products of glucosamine ractionated by organic solvent and thin layer chromatography. Nippon Shokuhin Gakk 1988; 35: 771-5.
- 17. Runyoro D, Ngassapa O, Vagionas K, Aligiannis N, Graikou K, Chinou I. Chemical composition and antimicrobial activity of the essential oils of four Ocimum species growing in Tanzania. Food Chem 2010 119: 311-6.
- 18. Stajner D, Milic N, Mimica-Dukic N, Lazic B, Igic R. Antioxidant abilities of cultivated and wild species of garlic. Phytotherapy Res 1998; 12: 13-4.
- 19. Malencic D, Gasiç O, Popovic M, Boza P. Screening for antioxidant properties of Salvia reflexa Hornem. Phytother Res 2000; 14: 546-8.
- 20. Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994; 315: 161-9.
- 21. Ulukanli Z, Karaborklu S, Bozok F, Ates B, Erdogan S, Cenet M, Karaaslan MG. Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils. Chin J Nat Med 2014; 12: 901-10.
- 22. Miri R, Firuzi O, Peymani P, Nazarian Z, Shafiee A. Synthesis and Cytotoxicity Study of New Cyclopenta [b] quinoline-1,8- dione Derivatives. Iran J PharmRes 2011;10:489–96.
Toplam 22 adet kaynakça vardır.
Ayrıntılar
| Konular | Sağlık Kurumları Yönetimi |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 11 Mayıs 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 21 Sayı: 3 |