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Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi

Yıl 2019, Cilt: 9 Sayı: 4, 2216 - 2224, 01.12.2019

Öz

Bu çalışmada üzüm çekirdeği ekstraktının Saccharomyces cerevisiae’de hidrojen peroksit hasarına karşı koruyucu etkisinin olup olmadığı moleküler biyolojik yönden araştırılmıştır. Bu araştırmada 4 grup oluşturulmuştur. Gruplar: (i) Kontrol grubu; (ii) Üzüm çekirdeği ekstraktı (ÜÇE) grubu; (iii) H2O2 grubu; (iv) ÜÇE + H2O2 grubu. S. cerevisiae kültürleri 1 saat, 3 saat, 5 saat ve 24 saat boyunca 30 °C'de geliştirildi. Hücre gelişimi ve lipit peroksidasyonu MDA (malondialdehit) analizleri spektrofotometre ile belirlendi. Total protein değişiklikleri SDS-PAGE elektroforezi ile tespit edildi ve Bradford metodu ile hesaplandı. Elde edilen sonuçlara göre; H2O2 grubu ile kıyaslandığında, ÜÇE+H2O2 grubunda (1, 3, 5 ve 24 saat) hücre gelişimi ve total protein sentezi artarken, MDA düzeyi azalış göstermiştir. Sonuç olarak üzüm çekirdeğinin S. cerevisiae kültüründe oksidatif hasarı azaltmasının yanı sıra, hücre büyümesini ve total protein sentezini teşvik edici bir role sahiptir.

Kaynakça

  • Aslan, 2006. Plasmodium vivax'ın laktat dehidrogenaz enzimini kodlayan genin ifade edilerek ilgili proteinin saflaştırılması ve analizlerinin yapılması, Fırat Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi (Basılmış).
  • Aslan A, 2015. The Effects of Different Essential FJ and their Combination on Saccharomyces cerevisiae Cell Growth. Progress in Nutrition, 17 (1): 36-40.
  • Aslan A, 2018. Cell Culture Developing and the İmaging of Total Protein Product Changing with SDS-PAGE in Saccharomyces cerevisiae. Progress Nutrition, 20 (1): 128-132.
  • Aslan A, Baspinar S, Yilmaz O, 2014b. Is Pomegranate Juice has a Vital Role for Protective Effect on Saccharomyces cerevisiae Growth?. Progress in Nutrition, 16 (3): 212-217.
  • Aslan A, Can MI, 2015a. The inhbition of Chromium Effect in Saccharomyces cerevisiae Thrive from Grapefruit. Progress in Nutrition, 17 (4): 339-342.
  • Aslan A, Can MI, 2015b.The Effect of Orange Juice against to H2O2 Stress in Saccharomyces cerevisiae. Progress in Nutrition, 17 (3): 250-254.
  • Aslan A, Can MI, Boydak D, 2014a. Anti-Oxidant Effects of Pomegranate Juice on Saccharomyces cerevisiae Cell Growth. Afr J Tradit Complement Altern Med, 11 (4): 14-18.
  • Aslan A, Gök Ö, Erman O, 2017. The Protective Effect of Kiwi Fruit Extract against to Chromium Effect on Protein Expression in Saccharomyces cerevisiae. Progress in Nutrition, 19(4): 472-476.
  • Aslan A, Gök Ö, Erman O, Kuloğlu T, 2018. Ellagic Acid İmpedes Carbontetrachloride-İnduced Liver Damage in Rats Through Suppression of NF-kB, Bcl-2 and Regulating Nrf-2 and Caspase Pathway. Biomedicine & Pharmacotherapy, 105: 662–669.
  • Aslan A. Can MI, 2017. Protein Expression Product Alterations in Saccharomyces cerevisiae. Progress in Nutrition, 19 (1): 81-85.
  • Aybastıer Ö, Dawbaa S, Demir C, 2018. Investigation of Antioxidant Ability Of Grape Seeds Extract to Prevent Oxidatively Induced DNA Damage by Gas Chromatography-Tandem Mass Spectrometry. Journal of Chromatography B, 1072: 328-335.
  • Babele PK, Thakre PK, Kumawat R, Tomar RS, 2018. Zinc Oxide Nanoparticles Induce Toxicity by Affecting Cell Wall Integrity Pathway, Mitochondrial Function and Lipid Homeostasis in Saccharomyces cerevisiae. Chemosphere, 213: 65-75.
  • Choudhary DK, Mishra A, 2018. In vitro investigation of Hypoglycemic and Oxidative Stress Properties of Fava Bean (Vicia faba L.) Seed Extract in Saccharomyces cerevisiae 2376. Preparative Biochemistry and Biotechnology, 48 (10): 920-929.
  • Eldaim MAA, Tousson E, El Sayed IET, El Aleim HA and Elsharkawy HN, 2019. Grape seeds proanthocyanidin extract ameliorates ehrlich solid tumor induced renal tissue and DNA damage in mice. Biomedicine & Pharmacotherapy, 115: 108908.
  • Göçmez A, Seferoğlu, HG, 2014. Asmalarda Resveratrol İçeriğini Etkileyen Faktörler ve İnsan Sağlığına Faydaları. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 11 (1): 31 – 38.
  • Hasona N and Morsi A, 2019. Grape seed extract alleviates dexamethasone-induced hyperlipidemia, lipid peroxidation, and hematological alteration in rats. Indian Journal of Clinical Biochemistry, 34 (2): 213-218.
  • Izadpanah A, Soorgi S and Geraminejad N, 2019. Effect of grape seed extract ointment on cesarean section wound healing: A double-blind, randomized, controlled clinical trial. Complementary Therapies in Clinical Practice 35: 323-328.
  • Jacewicz D, Siedlecka-Kroplewska K., Drzeżdżon J, Piotrowska A, Wyrzykowski D, Tesmar A, Zamojc K, Chmurzyński L, 2017. Method for Detection of Hydrogen Peroxide in HT22 Cells. Scientific Reports, 7: 45673.
  • Laemmli UK, 1970. Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4. Nature, 227: 680-685.
  • Ohkawa H, Ohishi N, Yagi K, 1979. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analyses of Biochemichal, 95: 351-358.
  • Park YS, Namiesnik J, Vearasilp K, Leontowicz H, Leontowicz M, Barasch D, Nemirovski A, Trakhtenberg S, Gorinstein S, 2014. Bioactive Compounds and the Antioxidant Capacity in New Kiwi Fruit Cultivars. Food Chemistry, 165: 354–361.
  • Rajkumari J, Dyavaiah M, Sudharshan SJ, Busi S, 2018. Evaluation of in vivo Antioxidant Potential of Syzygium jambos (L.) Alston and Terminalia citrina Roxb. towards Oxidative Stress Response in Saccharomyces cerevisiae. J Food Sci Technol, 55 (11): 4432-4439.
  • Santa K, Kumazawa Y and Nagaoka I, 2019. The potential use of grape phytochemicals for preventing the development of intestine-related and subsequent inflammatory diseases. Endocrine, Metabolic & İmmune Disorders Drug Targets, doi: 10.2174/1871530319666190529105226, (in press).
  • Soquetta MB, Stefanello FS, Mota Huerta K, Monteiro SS, Rosa CS, Terra NN, 2016. Characterization of Physiochemical and Microbiological Properties, and Bioactive Compounds, of Flour Made from the Skin and Bagasse of Kiwi Fruit (Actinidia deliciosa). Food chemistry, 199: 471-478.
  • Sun Q, Jia N, Li X, Yang J and Chen, G, 2019. Grape seed proanthocyanidins ameliorate neuronal oxidative damage by inhibiting GSK-3β-dependent mitochondrial permeability transition pore opening in an experimental model of sporadic Alzheimer's disease. Aging, 11(12):4107-4124.
  • Targhi RG, Banaei A and Saba V, 2019. Radioprotective effect of grape seed extract against gamma irradiation in mouse bone marrow cells. Journal of Cancer Research and Therapeutics, 15 (3): 512-516.
  • Tu X, Wang M, Liu Y, Zhao W, Ren X, Li Y, Liu H, Gu Z, Jia H and Li G, 2019. Pretreatment of grape seed proanthocyanidin extract exerts neuroprotective effect in murine model of neonatal hypoxic-ischemic brain ınjury by ıts antiapoptotic property. Cellular and Molecular Neurobiology, 1-9.
  • Vishvakarma R, Mishra A, 2019. Protective Effect of a Protease İnhibitor from Agaricus bisporus on Saccharomyces cerevisiae Cells against Oxidative Stress. Preparative Biochemistry and Biotechnology, 1-11.
  • Zhang R, Yu Q, Lu W, Shen J, Zhou D, Wang Y, Gao S and Wang Z, 2019. Grape seed procyanidin B2 promotes the autophagy and apoptosis in colorectal cancer cells via regulating PI3K/Akt signaling pathway. OncoTargets and Therapy, 12: 4109-4118.
  • Youssef S, Brisson G, Doucet-Beaupré H, Castonguay AM, Gora C, Amri M and Lévesque M, 2019. Neuroprotective benefits of grape seed and skin extract in a mouse model of Parkinson’s disease. Nutritional Neuroscience, doi: 10.1080/1028415X.2019.1616435, (in press).

The Protective Effect of Grape Seed Extract Against to Hydrogen Peroxide -Induced Damage in Saccharomyces cerevisiae

Yıl 2019, Cilt: 9 Sayı: 4, 2216 - 2224, 01.12.2019

Öz

In this study, it was investigated whether grape seed extract had a protective effect against hydrogen peroxide damage in Saccharomyces cerevisiae the aspect of molecular biology. In this study 4 groups were formed. Groups: (i) Control group; (ii) Grape seed extract group; (iii) H2O2 group; (iv) Grape seed extract + H2O2 group. S. cerevisiae cultures were developed at 30 ° C for 1 hour, 3 hours, 5 hours and 24 hours. Cell development and lipid peroxidation MDA (malondialdehyde) analyzes were determined by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and calculated by the Bradford method. According to the results, cell development and total protein synthesis increased in the ÜÇE + H2O2 group (1, 3, 5 and 24 hours) and MDA level decreased compared to the H2O2 group. As a result, grape seed extract has a role in promoting cell growth and total protein synthesis as well as reducing oxidative damage in S. cerevisiae culture.

Kaynakça

  • Aslan, 2006. Plasmodium vivax'ın laktat dehidrogenaz enzimini kodlayan genin ifade edilerek ilgili proteinin saflaştırılması ve analizlerinin yapılması, Fırat Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi (Basılmış).
  • Aslan A, 2015. The Effects of Different Essential FJ and their Combination on Saccharomyces cerevisiae Cell Growth. Progress in Nutrition, 17 (1): 36-40.
  • Aslan A, 2018. Cell Culture Developing and the İmaging of Total Protein Product Changing with SDS-PAGE in Saccharomyces cerevisiae. Progress Nutrition, 20 (1): 128-132.
  • Aslan A, Baspinar S, Yilmaz O, 2014b. Is Pomegranate Juice has a Vital Role for Protective Effect on Saccharomyces cerevisiae Growth?. Progress in Nutrition, 16 (3): 212-217.
  • Aslan A, Can MI, 2015a. The inhbition of Chromium Effect in Saccharomyces cerevisiae Thrive from Grapefruit. Progress in Nutrition, 17 (4): 339-342.
  • Aslan A, Can MI, 2015b.The Effect of Orange Juice against to H2O2 Stress in Saccharomyces cerevisiae. Progress in Nutrition, 17 (3): 250-254.
  • Aslan A, Can MI, Boydak D, 2014a. Anti-Oxidant Effects of Pomegranate Juice on Saccharomyces cerevisiae Cell Growth. Afr J Tradit Complement Altern Med, 11 (4): 14-18.
  • Aslan A, Gök Ö, Erman O, 2017. The Protective Effect of Kiwi Fruit Extract against to Chromium Effect on Protein Expression in Saccharomyces cerevisiae. Progress in Nutrition, 19(4): 472-476.
  • Aslan A, Gök Ö, Erman O, Kuloğlu T, 2018. Ellagic Acid İmpedes Carbontetrachloride-İnduced Liver Damage in Rats Through Suppression of NF-kB, Bcl-2 and Regulating Nrf-2 and Caspase Pathway. Biomedicine & Pharmacotherapy, 105: 662–669.
  • Aslan A. Can MI, 2017. Protein Expression Product Alterations in Saccharomyces cerevisiae. Progress in Nutrition, 19 (1): 81-85.
  • Aybastıer Ö, Dawbaa S, Demir C, 2018. Investigation of Antioxidant Ability Of Grape Seeds Extract to Prevent Oxidatively Induced DNA Damage by Gas Chromatography-Tandem Mass Spectrometry. Journal of Chromatography B, 1072: 328-335.
  • Babele PK, Thakre PK, Kumawat R, Tomar RS, 2018. Zinc Oxide Nanoparticles Induce Toxicity by Affecting Cell Wall Integrity Pathway, Mitochondrial Function and Lipid Homeostasis in Saccharomyces cerevisiae. Chemosphere, 213: 65-75.
  • Choudhary DK, Mishra A, 2018. In vitro investigation of Hypoglycemic and Oxidative Stress Properties of Fava Bean (Vicia faba L.) Seed Extract in Saccharomyces cerevisiae 2376. Preparative Biochemistry and Biotechnology, 48 (10): 920-929.
  • Eldaim MAA, Tousson E, El Sayed IET, El Aleim HA and Elsharkawy HN, 2019. Grape seeds proanthocyanidin extract ameliorates ehrlich solid tumor induced renal tissue and DNA damage in mice. Biomedicine & Pharmacotherapy, 115: 108908.
  • Göçmez A, Seferoğlu, HG, 2014. Asmalarda Resveratrol İçeriğini Etkileyen Faktörler ve İnsan Sağlığına Faydaları. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 11 (1): 31 – 38.
  • Hasona N and Morsi A, 2019. Grape seed extract alleviates dexamethasone-induced hyperlipidemia, lipid peroxidation, and hematological alteration in rats. Indian Journal of Clinical Biochemistry, 34 (2): 213-218.
  • Izadpanah A, Soorgi S and Geraminejad N, 2019. Effect of grape seed extract ointment on cesarean section wound healing: A double-blind, randomized, controlled clinical trial. Complementary Therapies in Clinical Practice 35: 323-328.
  • Jacewicz D, Siedlecka-Kroplewska K., Drzeżdżon J, Piotrowska A, Wyrzykowski D, Tesmar A, Zamojc K, Chmurzyński L, 2017. Method for Detection of Hydrogen Peroxide in HT22 Cells. Scientific Reports, 7: 45673.
  • Laemmli UK, 1970. Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4. Nature, 227: 680-685.
  • Ohkawa H, Ohishi N, Yagi K, 1979. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analyses of Biochemichal, 95: 351-358.
  • Park YS, Namiesnik J, Vearasilp K, Leontowicz H, Leontowicz M, Barasch D, Nemirovski A, Trakhtenberg S, Gorinstein S, 2014. Bioactive Compounds and the Antioxidant Capacity in New Kiwi Fruit Cultivars. Food Chemistry, 165: 354–361.
  • Rajkumari J, Dyavaiah M, Sudharshan SJ, Busi S, 2018. Evaluation of in vivo Antioxidant Potential of Syzygium jambos (L.) Alston and Terminalia citrina Roxb. towards Oxidative Stress Response in Saccharomyces cerevisiae. J Food Sci Technol, 55 (11): 4432-4439.
  • Santa K, Kumazawa Y and Nagaoka I, 2019. The potential use of grape phytochemicals for preventing the development of intestine-related and subsequent inflammatory diseases. Endocrine, Metabolic & İmmune Disorders Drug Targets, doi: 10.2174/1871530319666190529105226, (in press).
  • Soquetta MB, Stefanello FS, Mota Huerta K, Monteiro SS, Rosa CS, Terra NN, 2016. Characterization of Physiochemical and Microbiological Properties, and Bioactive Compounds, of Flour Made from the Skin and Bagasse of Kiwi Fruit (Actinidia deliciosa). Food chemistry, 199: 471-478.
  • Sun Q, Jia N, Li X, Yang J and Chen, G, 2019. Grape seed proanthocyanidins ameliorate neuronal oxidative damage by inhibiting GSK-3β-dependent mitochondrial permeability transition pore opening in an experimental model of sporadic Alzheimer's disease. Aging, 11(12):4107-4124.
  • Targhi RG, Banaei A and Saba V, 2019. Radioprotective effect of grape seed extract against gamma irradiation in mouse bone marrow cells. Journal of Cancer Research and Therapeutics, 15 (3): 512-516.
  • Tu X, Wang M, Liu Y, Zhao W, Ren X, Li Y, Liu H, Gu Z, Jia H and Li G, 2019. Pretreatment of grape seed proanthocyanidin extract exerts neuroprotective effect in murine model of neonatal hypoxic-ischemic brain ınjury by ıts antiapoptotic property. Cellular and Molecular Neurobiology, 1-9.
  • Vishvakarma R, Mishra A, 2019. Protective Effect of a Protease İnhibitor from Agaricus bisporus on Saccharomyces cerevisiae Cells against Oxidative Stress. Preparative Biochemistry and Biotechnology, 1-11.
  • Zhang R, Yu Q, Lu W, Shen J, Zhou D, Wang Y, Gao S and Wang Z, 2019. Grape seed procyanidin B2 promotes the autophagy and apoptosis in colorectal cancer cells via regulating PI3K/Akt signaling pathway. OncoTargets and Therapy, 12: 4109-4118.
  • Youssef S, Brisson G, Doucet-Beaupré H, Castonguay AM, Gora C, Amri M and Lévesque M, 2019. Neuroprotective benefits of grape seed and skin extract in a mouse model of Parkinson’s disease. Nutritional Neuroscience, doi: 10.1080/1028415X.2019.1616435, (in press).
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Yapısal Biyoloji
Bölüm Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic
Yazarlar

Abdullah Aslan 0000-0002-6243-4221

Özlem Gök 0000-0001-8521-6369

Seda Beyaz 0000-0003-0436-8112

Yayımlanma Tarihi 1 Aralık 2019
Gönderilme Tarihi 12 Mayıs 2019
Kabul Tarihi 8 Temmuz 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 9 Sayı: 4

Kaynak Göster

APA Aslan, A., Gök, Ö., & Beyaz, S. (2019). Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi. Journal of the Institute of Science and Technology, 9(4), 2216-2224.
AMA Aslan A, Gök Ö, Beyaz S. Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi. Iğdır Üniv. Fen Bil Enst. Der. Aralık 2019;9(4):2216-2224.
Chicago Aslan, Abdullah, Özlem Gök, ve Seda Beyaz. “Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi”. Journal of the Institute of Science and Technology 9, sy. 4 (Aralık 2019): 2216-24.
EndNote Aslan A, Gök Ö, Beyaz S (01 Aralık 2019) Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi. Journal of the Institute of Science and Technology 9 4 2216–2224.
IEEE A. Aslan, Ö. Gök, ve S. Beyaz, “Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi”, Iğdır Üniv. Fen Bil Enst. Der., c. 9, sy. 4, ss. 2216–2224, 2019.
ISNAD Aslan, Abdullah vd. “Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi”. Journal of the Institute of Science and Technology 9/4 (Aralık 2019), 2216-2224.
JAMA Aslan A, Gök Ö, Beyaz S. Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi. Iğdır Üniv. Fen Bil Enst. Der. 2019;9:2216–2224.
MLA Aslan, Abdullah vd. “Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi”. Journal of the Institute of Science and Technology, c. 9, sy. 4, 2019, ss. 2216-24.
Vancouver Aslan A, Gök Ö, Beyaz S. Üzüm Çekirdeği Ekstraktının Saccharomyces cerevisiae’de Oluşturulan Hidrojen Peroksit Hasarına Karşı Koruyucu Etkisi. Iğdır Üniv. Fen Bil Enst. Der. 2019;9(4):2216-24.