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Protective Role of Resveratrol Against Toluene-Induced Oxidative Stress in Rats

Year 2024, Volume: 45 Issue: 2, 188 - 193, 30.06.2024

Ahmet Barat , Egemen Dere

https://doi.org/10.17776/csj.1409301
Cited By: 1

Abstract

Toluene is a toxic substance commonly used in the industry that leads to oxidative stress in the body and causes in the formation of reactive oxygen species. In addition to the antioxidants produced by our body, we also obtain antioxidants through foods. Resveratrol is a polyphenol found in many plant species and possesses antioxidant properties. The effects of oxidative stress induced by toluene and the protective effect of resveratrol were investigated through changes in malondialdehyde (MDA) and glutathione (GSH) levels. Male rats were administered different doses of toluene and resveratrol (5 mg/kg, 10 mg/kg, 20 mg/kg), while control groups were given physiological saline and ethanol. At the end of the experiment, blood and lung tissues of the animals were examined, and MDA and GSH levels were measured. According to the obtained data, a significant increase in GSH activity was observed after the administration of toluene. However, in groups treated with combinations of toluene and various doses of resveratrol, a significant decrease in GSH levels was detected. While a significant increase in MDA levels in lung tissues was observed between the control groups and the toluene group, no significant change was observed in blood samples.

Keywords

Toluene Resveratrol Malondialdehyde Glutathione

Ethical Statement

Bu çalışma Bursa Uludağ Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir (QUAP(F)-2019/14). Deneyler, hayvan deneyleri yerel etik kurulundan alınan 2019/04-03 numaralı etik kurul kararına uygun olarak ve 2013 yılında revize edilen Helsinki Deklarasyonuna uyularak gerçekleştirilmiştir

Supporting Institution

Bursa Uludağ Üniversitesi Bilimsel Araştırmalar Birimi

Project Number

QUAP(F)-2019/14.

Thanks

Makale, Uludağ Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Moleküler Biyoloji Bilim Dalında Ahmad Baratov tarafından tamamlanan "Resveratrolün Toluen Maruziyetine Bağlı Oksidatif Stres Etkilerine Karşı Koruyucu Özelliklerinin Araştırılması" başlıklı yüksek lisans tezine dayanmaktadır. Bu çalışmaya katkılarından dolayı Emine Gonca PEKEL, Oğuzhan Akgün ve Raissa SOAMANJARY'ye teşekkür ederiz.

References

  • [1] Mohammad M.F., Mousa L.Y., Idayyir D.H., Kadim Z.M., The Toxic Effects of Toluene on Oxidative Stress Status in the Genital System of Female Mice, TMJ., 44 (6) (2021) 3345-3353.
  • [2] Özcan O., Erdal H., Çakırca G., Yönden Z., OxidativeS and Its Impacts on Intracellular Lipids, Proteins and DNA, JCEI., 6 (3) (2015) 331-336
  • [3] Cederbaum A.I., Cytochrome P450 2E1-Dependent Oxidant Stress and Upregulation of Anti-Oxidant Defense in Liver Cells, J Gastroenterol Hepatol., 21 (2006) 22–S5.
  • [4] Finaud J., Lac G., Filaire E., Oxidative Stress, Sports Med., 36 (4) (2006) 327-358.
  • [5] Filley C.M., Halliday W., Kleinschmidt-Demasters, B.K., The Effects of Toluene on The Central Nervous System, J Neuropathol Exp Neurol., 63 (1) (2004) 1-12.
  • [6] Jadimurthy R., Jagadish S., NayakS.C., Kumar S., Mohan C.D., Rangappa K.S., Phytochemicals as Invaluable Sources of Potent Antimicrobial Agents to Combat Antibiotic Resistance, Life, 13 (2023), 948.
  • [7] Wallerath T., Deckert G., Ternes T., Anderson H., Li H., Witte K., Förstermann U., Resveratrol, aPolyphenolic Phytoalexin Present in Red Wine, Enhances Expression and Activity of Endothelial Nitric Oxide Synthase, Circulation, 106 (13) (2002) 1652-1658.
  • [8] Montero C., Cristescu S.M., Jiménez J.B., Orea J.M., Hekkert S.L., Harren F.J.M., Ureña A.G., trans-Resveratrol and Grape Disease Resistance. A Dynamical Study by High-Resolution Laser-Based Techniques, Plant Physiol., 131 (1) (2003) 129–138.
  • [9] Xia N., Daiber A., Förstermann U., Li H., Antioxidant Effects of Resveratrol in the Cardiovascular System, British J Pharmacol., 174 (12) (2017) 1633–1646.
  • [10] Leifert W.R., Abeywardena M.Y., Cardioprotective Actions of Grape Polyphenols, Nutr Res., 28 (2008) 729–37.
  • [11] Lushchak V.I., Glutathione Homeostasis and Functions: Potential Targets for Medical Interventions, J. Amino Acids. 2012 (2012) 26
  • [12] Wu G., Fang Y.Z., Yang S., Lupton J.R., Turner N.D., Glutathione Metabolism and its Implications for Health, J Nutr., 134 (2004) 489–492.
  • [13] Cheng S.B., Liu H.T., Chen S.Y., Lin P.T., Lai C. Y., Huang Y.V., Changes of Oxidative Stress, Glutathione, and Its Dependent Antioxidant Enzyme Activities in Patients with Hepatocellular Carcinoma before and after Tumor Resection, PLoS One, 12(1) (2017)
  • [14] Tsikas D., Assessment o Lipid Peroxidation by Measuring Malondialdehyde (MDA) and Relatives in Biological Samples: Analytical and biological challenges, Anal Biochem., 524 (2017) 13-30.
  • [15] Danuta R., Juan D.S., Gabriella W., Mohan R., Ioli M., Stefan P., Jean O., Samuel D. Lipid Profile Analysis in Spinal Trauma Patients Shows Severe Distortion of AA/DHA after Injury, Front Immunol, 4 (2013)
  • [16] Çiçek M., Şişman T., Akut Tolüen Maruziyetinin Tavşan Beyin Korteksinde Yol Açtığı Toksik Etkiler, Etlik Vet Mikrobiyol Derg., 28 (1) (2017) 33-38.
  • [17] Ulakoğlu Z. E., Saygı A., Gümüştaş M.K., Zor E., Akkaya A., Kökoğlu E., The efEfect of Chronic Thinner İnhalation on Lipid Peroxidation in Rat Lung and Liver, Cerrahpaşa J. Med, 29 (2) (1998) 75-78.
  • [18] Choi G.S., Trinh H.K.T., Yang E.M., Ye Y.M., Shin Y.S., Kim S.H., Park H.S. Role of Clusterin/Progranulin in Toluene Diisocyanate-İnduced Occupational Asthma, Exp Mol Med, 50 (5) (2018).
  • [19] Park J.J., Chang H., Jung J.Y., Jung S.J., Song K.H., Suh W.N., Chun S.W., Lee H. Y.Yoo T.H., A Case of Tolüene-induced Renal Tubular Acidosis Presented with Hypokalemic Paralysis, JKSEM, 17 (6) (2006) 656-658.
  • [20] Chavan S., Sava L., Saxena V., Pillai S., Sontakke A., Ingole D., Reduced Glutathione: Importance of Specimen Collection, Indian J Clin Biochem, 20 (1) (2005) 150-152.
  • [21] Das D.K., Maulik N. Resveratrol in Cardioprotection: a Therapeutic Promise of Alternative Medicine, Mol. Interv., 6 (1) (2006) 36-47.
  • [22] Bircan C.I., Merhan O., Kadmiyum Uygulanan Farelerde Oluşturulan Oksidatif Strese Karşı Resveratrolün Koruyucu Etkisinin Araştırılması, Erciyes Üniv. Vet. Fak. Derg., 17 (3) (2020) 215-220.

Year 2024, Volume: 45 Issue: 2, 188 - 193, 30.06.2024

Ahmet Barat , Egemen Dere

https://doi.org/10.17776/csj.1409301
Cited By: 1

Abstract

Project Number

QUAP(F)-2019/14.

References

  • [1] Mohammad M.F., Mousa L.Y., Idayyir D.H., Kadim Z.M., The Toxic Effects of Toluene on Oxidative Stress Status in the Genital System of Female Mice, TMJ., 44 (6) (2021) 3345-3353.
  • [2] Özcan O., Erdal H., Çakırca G., Yönden Z., OxidativeS and Its Impacts on Intracellular Lipids, Proteins and DNA, JCEI., 6 (3) (2015) 331-336
  • [3] Cederbaum A.I., Cytochrome P450 2E1-Dependent Oxidant Stress and Upregulation of Anti-Oxidant Defense in Liver Cells, J Gastroenterol Hepatol., 21 (2006) 22–S5.
  • [4] Finaud J., Lac G., Filaire E., Oxidative Stress, Sports Med., 36 (4) (2006) 327-358.
  • [5] Filley C.M., Halliday W., Kleinschmidt-Demasters, B.K., The Effects of Toluene on The Central Nervous System, J Neuropathol Exp Neurol., 63 (1) (2004) 1-12.
  • [6] Jadimurthy R., Jagadish S., NayakS.C., Kumar S., Mohan C.D., Rangappa K.S., Phytochemicals as Invaluable Sources of Potent Antimicrobial Agents to Combat Antibiotic Resistance, Life, 13 (2023), 948.
  • [7] Wallerath T., Deckert G., Ternes T., Anderson H., Li H., Witte K., Förstermann U., Resveratrol, aPolyphenolic Phytoalexin Present in Red Wine, Enhances Expression and Activity of Endothelial Nitric Oxide Synthase, Circulation, 106 (13) (2002) 1652-1658.
  • [8] Montero C., Cristescu S.M., Jiménez J.B., Orea J.M., Hekkert S.L., Harren F.J.M., Ureña A.G., trans-Resveratrol and Grape Disease Resistance. A Dynamical Study by High-Resolution Laser-Based Techniques, Plant Physiol., 131 (1) (2003) 129–138.
  • [9] Xia N., Daiber A., Förstermann U., Li H., Antioxidant Effects of Resveratrol in the Cardiovascular System, British J Pharmacol., 174 (12) (2017) 1633–1646.
  • [10] Leifert W.R., Abeywardena M.Y., Cardioprotective Actions of Grape Polyphenols, Nutr Res., 28 (2008) 729–37.
  • [11] Lushchak V.I., Glutathione Homeostasis and Functions: Potential Targets for Medical Interventions, J. Amino Acids. 2012 (2012) 26
  • [12] Wu G., Fang Y.Z., Yang S., Lupton J.R., Turner N.D., Glutathione Metabolism and its Implications for Health, J Nutr., 134 (2004) 489–492.
  • [13] Cheng S.B., Liu H.T., Chen S.Y., Lin P.T., Lai C. Y., Huang Y.V., Changes of Oxidative Stress, Glutathione, and Its Dependent Antioxidant Enzyme Activities in Patients with Hepatocellular Carcinoma before and after Tumor Resection, PLoS One, 12(1) (2017)
  • [14] Tsikas D., Assessment o Lipid Peroxidation by Measuring Malondialdehyde (MDA) and Relatives in Biological Samples: Analytical and biological challenges, Anal Biochem., 524 (2017) 13-30.
  • [15] Danuta R., Juan D.S., Gabriella W., Mohan R., Ioli M., Stefan P., Jean O., Samuel D. Lipid Profile Analysis in Spinal Trauma Patients Shows Severe Distortion of AA/DHA after Injury, Front Immunol, 4 (2013)
  • [16] Çiçek M., Şişman T., Akut Tolüen Maruziyetinin Tavşan Beyin Korteksinde Yol Açtığı Toksik Etkiler, Etlik Vet Mikrobiyol Derg., 28 (1) (2017) 33-38.
  • [17] Ulakoğlu Z. E., Saygı A., Gümüştaş M.K., Zor E., Akkaya A., Kökoğlu E., The efEfect of Chronic Thinner İnhalation on Lipid Peroxidation in Rat Lung and Liver, Cerrahpaşa J. Med, 29 (2) (1998) 75-78.
  • [18] Choi G.S., Trinh H.K.T., Yang E.M., Ye Y.M., Shin Y.S., Kim S.H., Park H.S. Role of Clusterin/Progranulin in Toluene Diisocyanate-İnduced Occupational Asthma, Exp Mol Med, 50 (5) (2018).
  • [19] Park J.J., Chang H., Jung J.Y., Jung S.J., Song K.H., Suh W.N., Chun S.W., Lee H. Y.Yoo T.H., A Case of Tolüene-induced Renal Tubular Acidosis Presented with Hypokalemic Paralysis, JKSEM, 17 (6) (2006) 656-658.
  • [20] Chavan S., Sava L., Saxena V., Pillai S., Sontakke A., Ingole D., Reduced Glutathione: Importance of Specimen Collection, Indian J Clin Biochem, 20 (1) (2005) 150-152.
  • [21] Das D.K., Maulik N. Resveratrol in Cardioprotection: a Therapeutic Promise of Alternative Medicine, Mol. Interv., 6 (1) (2006) 36-47.
  • [22] Bircan C.I., Merhan O., Kadmiyum Uygulanan Farelerde Oluşturulan Oksidatif Strese Karşı Resveratrolün Koruyucu Etkisinin Araştırılması, Erciyes Üniv. Vet. Fak. Derg., 17 (3) (2020) 215-220.
There are 22 citations in total.

Details

Primary Language English
Subjects Enzymes
Journal Section Natural Sciences
Authors

Ahmet Barat 0000-0002-7618-9626

Egemen Dere 0000-0001-9572-1051

Project Number QUAP(F)-2019/14.
Publication Date June 30, 2024
Submission Date January 17, 2024
Acceptance Date June 13, 2024
Published in Issue Year 2024Volume: 45 Issue: 2

Cite

APA Barat, A., & Dere, E. (2024). Protective Role of Resveratrol Against Toluene-Induced Oxidative Stress in Rats. Cumhuriyet Science Journal, 45(2), 188-193. https://doi.org/10.17776/csj.1409301

Cited By

Combined effects of benzene, toluene, xylene, ethylbenzene, and styrene exposure on hearing loss mediated by oxidative stress at realistic low levels
Environmental Pollution
https://doi.org/10.1016/j.envpol.2024.125149
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