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Crocus Officinalis (L.) Extract on Human Colerectal Cancer Cell Line (HT-22): Investigation in Vitro

Year 2024, Volume: 45 Issue: 1, 35 - 39, 28.03.2024

Sinan Soylu

https://doi.org/10.17776/csj.1419826

Abstract

Crocus sativus L. has been used as spice, food colouring and medicinal plant for thousands of years. In this study, antioxidant properties and cytotoxic effect of saffron extract were evaluated in HT-22 cell lines. Malignant and non-malignant cells (L929) were cultured in DMEM medium and incubated with different concentrations of saffron extract in different solvents (water, ethanol, ethyl-acetate, n-hexane). Cell viability was determined by MTT assay. Antioxidant parameters such as DPPH, FRAP, total polyphenol and flavonoid amounts were measured. The highest DPPH value was found as 15.90±0.12 µg/mL in ethyl acetate solvent. Total polyphenols were 89.06±0.02 mg GAE/g extract, flavonoids were 76.98±2.08 µg QE/g extract and FRAP was 67.93±0.98 mg/g extract in ethanol solvent. In colon cancer, IC50 value 94±1.37 µg/mL was found to be the highest value. This is thought to be due to the high antioxidant capacity in ethanol solvent. While antioxidant capacity was not found to be high, antiproliferation effect was found to be high. Saffron may also be considered as a promising chemotherapeutic agent in cancer treatment in the future.

Keywords

Antioxidant parameters Crocus sativus L. HT-22 cell lines Saffron

References

  • [1] Smith-Warner S.A., Elmer P.J., Tharp T.M., Fosdick L., Randall B., Gross M., Wood J., Potter J.D., Increasing vegetable and fruit intake: randomized intervention and monitoring in an at-risk population, Cancer Epidemiol Biomar Prev., 9 (3) (2000) 307-317.
  • [2] World Health Organization. 2018. Global Health Observatory. http: //www.who.int.- /data/gho Erişim tarihi: 21.06.2018
  • [3] Doğruyol H., Additives in Foods and Their Harm; Childhood Hyperactivity, Current Pediatrics., 4 (2) (2006) 42-48.
  • [4] Abdullaev F.I., Espinosa-Aguirre J.J., Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials, Cancer Detection and Prevention., 28 (6) (2004) 426-432.
  • [5] Khazdair M.R., Boskabady M.H., Hosseini M., Rezaee R., Tsatsakis A.M., The effects of Crocus sativus (saffron) and its constituents on nervous system: A review, Avicenna J Phytomed., 5 (5) (2015) 376-391.
  • [6] Behzad S., Razavi M., Mahajeri M., The effect of mineral nutrients (N.P.K.) on saffron production, Acta Hort., 306 (1992) 426-430.
  • [7] Melnyk J.P., Wang S., Marcone M.F., Chemical and biological properties of the world's most expensive spice: Saffron, Food Research International., 43 (8) (2010) 1981– 1989.
  • [8] Bolhassani A., Khavari A., Bathaie S.Z., Saffron and natural carotenoids: Biochemical activities and anti-tumor effects, Biochimica et Biophysica Acta (BBA)-Reviews on Cancer., 1845 (1 )(2014) 20-30.
  • [9] Rios, J. L., Recio, M. C., Giner, R. M. ve Manez, S. (1996). An Update Review of Saffron and İts Active Constituents. Phytotherapy Research, 10(3), 189-193.
  • [10] Zararsız H. F., Gastronomi Ve Mutfak Sanatları Anabilim Dalı, master thesis, karabük of university, Lisansüstü Eğitim of institue, 2023.
  • [11] Abdullaev F.I., Riverón-Negrete L., Caballero-Ortega H., Manuel Hernández J., Pérez-López I., Pereda-Miranda R., Espinosa-Aguirre J.J., Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.), Toxicol In Vitro., 17 (5-6) (2003) 731-736.
  • [12] Hosseinzadeh H., Abootorabi A., Sadeghnia H.R., Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs, Dna and Cell Biology., 27 (12) (2008) 1-8.
  • [13] Assimopoulou A.N., Sinakos Z., Papageorgiou V.P., Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents, Phytother. Res., 19(11) (2005) 997-1000.
  • [14] Hosseinzadeh H., Younesi H.M., Antinociceptive and antiinflammatory effects of Crocus sativus L. stigma and petal extracts in mice, BMC Pharmacol., 2 (2002) 1-8.
  • [15] Fatehi M., Rashidabady T., Fatehi-Hassanabad Z., Effects of Crocus sativus petals' extract on rat blood pressure, J. Ethnopharmacol., 84 (2-3) (2003) 199-203.
  • [16] Mashmoul M., Azlan A., Yusof B.N.M., Khaza’ai H., Mohtarrudin N., Boroushaki M.T., Effects of saffron extract and crocin on anthropometrical, nutritional and lipid profile parameters of rats fed a high fat diet, Journal of Functional Foods, 8 (2014) 180-187.
  • [17] Omidi A., Riahinia N., Montazer Torbati M.B., Behdani M.A., Hepatoprotectiveeffect of Crocus sativus(saffron)petals extract against acetaminophen toxicity in male Wistar rats, Avicenna J Phytomed., 4 (5) (2014) 330- 336.
  • [18] Cuendet M., Hostettmann K., Potterat O., Iridoid glucosides with free radical scavenging properties from Fagraea blumei, Helvetica Chimica Acta, 80 (4) (1997) 1144-1152.
  • [19] Benzie I. F., Strain J. J., The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay, Analytical biochemistry., 239 (1) (1996) 70-76.
  • [20] Joseph J., Paul D., Kavitha M.P., Dineshkumar B., Menon J.S., Bhat A.R. and Krishnakumar K., Preliminary phytochemical screening and in vitro antioxidant activity of Banana flower (Musa paradisiaca AAB Nendran variety), Journal of Pharmacy Research, 8 (2) (2014) 144 – 1519.
  • [21] Chang C.C., Yang M.H., Wen H.M., Chern J.C., Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis, 10 (3) (2002) 178–182.
  • [22] Gezegen H., Tutar U., Hepokur C., Ceylan M., Synthesis and biological evaluation of novel indenopyrazole derivatives, J Biochem Mol Toxicol., 33(5) (2019) :e22285. doi: 10.1002/jbt.22285
  • [23] Kaur C., Dalamu M., Singh S., Walia S., Joshi A.D., Variations in phenolics and antioxidants in Indian onions (Allium cepa L.) Genotype selection for breeding, Nutr. Food Sci., 40 (1) (2004) 6–19
  • [24] Mzabri I., Addi M., Berrichi A., Traditional and Modern Uses of Saffron (Crocus Sativus), Cosmetics, 6 (4) (2019) 63.
  • [25] Somayeh R., Sohrab M., Maryam H., Seyed A.S., Evaluation of antioxidant activities of bioactive compounds and various extracts obtained from saffron (Crocus sativus L.): A review, J. Food Sci. Technol., 52 (2015) 1881–1888.
  • [26] Karimi E., Oskoueian E., Hendra R., Jaafar H.Z., Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity, Molecules, 15 (9) (2010) 6244–6256.
  • [27] Forni C., Facchiano F., Bartoli M., Beneficial Role of Phytochemicals on Oxidative Stress and Age-Related Diseases, BioMed Res. Int., 7 (2019) 1-16.
  • [28] Makhlouf H., Saksouk M., Habib J., Chahine R., Determination of antioxidant activity of saffron taken from the flower of Crocus sativus grown in Lebanon., African Journal of Biotechnology, 10 (41) (2011) 8093-8100.
  • [29] Hawa Z., Jaafari E., Bahtaei D., Evaluation of Crocus sativus L. Stigma Phenolic and Flavonoid Compounds and Its Antioxidant Activity, Molecules, 15 (9) (2010) 6244-6256.
  • [30] Tavakkol-Afshari J., Brook A., Mousavi S. H., Study of cytotoxic and apoptogenic properties of saffron extract in human cancer cell lines, Food and Chemical Toxicology, 46 (11) (2018) 3443-3447.
  • [31] Kondhare D., Lade H., Phytochemical profile, aldose reductase inhibitory, and antioxidant activities of indian traditional medicinal Coccinia grandis (L.) fruit extract, Biotech., 7 (6) (2017) 378.
  • [32] Wali A. F., Alchamat H. A. A., Hariri H. K., Hariri B. K., Menezes G. A., Zehra U., Ahmad, P., Antioxidant, antimicrobial, antidiabetic and cytotoxic activity of Crocus sativus L. Petals, Applied Sciences., 10 (4) (2020) 1519.

Year 2024, Volume: 45 Issue: 1, 35 - 39, 28.03.2024

Sinan Soylu

https://doi.org/10.17776/csj.1419826

Abstract

References

  • [1] Smith-Warner S.A., Elmer P.J., Tharp T.M., Fosdick L., Randall B., Gross M., Wood J., Potter J.D., Increasing vegetable and fruit intake: randomized intervention and monitoring in an at-risk population, Cancer Epidemiol Biomar Prev., 9 (3) (2000) 307-317.
  • [2] World Health Organization. 2018. Global Health Observatory. http: //www.who.int.- /data/gho Erişim tarihi: 21.06.2018
  • [3] Doğruyol H., Additives in Foods and Their Harm; Childhood Hyperactivity, Current Pediatrics., 4 (2) (2006) 42-48.
  • [4] Abdullaev F.I., Espinosa-Aguirre J.J., Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials, Cancer Detection and Prevention., 28 (6) (2004) 426-432.
  • [5] Khazdair M.R., Boskabady M.H., Hosseini M., Rezaee R., Tsatsakis A.M., The effects of Crocus sativus (saffron) and its constituents on nervous system: A review, Avicenna J Phytomed., 5 (5) (2015) 376-391.
  • [6] Behzad S., Razavi M., Mahajeri M., The effect of mineral nutrients (N.P.K.) on saffron production, Acta Hort., 306 (1992) 426-430.
  • [7] Melnyk J.P., Wang S., Marcone M.F., Chemical and biological properties of the world's most expensive spice: Saffron, Food Research International., 43 (8) (2010) 1981– 1989.
  • [8] Bolhassani A., Khavari A., Bathaie S.Z., Saffron and natural carotenoids: Biochemical activities and anti-tumor effects, Biochimica et Biophysica Acta (BBA)-Reviews on Cancer., 1845 (1 )(2014) 20-30.
  • [9] Rios, J. L., Recio, M. C., Giner, R. M. ve Manez, S. (1996). An Update Review of Saffron and İts Active Constituents. Phytotherapy Research, 10(3), 189-193.
  • [10] Zararsız H. F., Gastronomi Ve Mutfak Sanatları Anabilim Dalı, master thesis, karabük of university, Lisansüstü Eğitim of institue, 2023.
  • [11] Abdullaev F.I., Riverón-Negrete L., Caballero-Ortega H., Manuel Hernández J., Pérez-López I., Pereda-Miranda R., Espinosa-Aguirre J.J., Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.), Toxicol In Vitro., 17 (5-6) (2003) 731-736.
  • [12] Hosseinzadeh H., Abootorabi A., Sadeghnia H.R., Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs, Dna and Cell Biology., 27 (12) (2008) 1-8.
  • [13] Assimopoulou A.N., Sinakos Z., Papageorgiou V.P., Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents, Phytother. Res., 19(11) (2005) 997-1000.
  • [14] Hosseinzadeh H., Younesi H.M., Antinociceptive and antiinflammatory effects of Crocus sativus L. stigma and petal extracts in mice, BMC Pharmacol., 2 (2002) 1-8.
  • [15] Fatehi M., Rashidabady T., Fatehi-Hassanabad Z., Effects of Crocus sativus petals' extract on rat blood pressure, J. Ethnopharmacol., 84 (2-3) (2003) 199-203.
  • [16] Mashmoul M., Azlan A., Yusof B.N.M., Khaza’ai H., Mohtarrudin N., Boroushaki M.T., Effects of saffron extract and crocin on anthropometrical, nutritional and lipid profile parameters of rats fed a high fat diet, Journal of Functional Foods, 8 (2014) 180-187.
  • [17] Omidi A., Riahinia N., Montazer Torbati M.B., Behdani M.A., Hepatoprotectiveeffect of Crocus sativus(saffron)petals extract against acetaminophen toxicity in male Wistar rats, Avicenna J Phytomed., 4 (5) (2014) 330- 336.
  • [18] Cuendet M., Hostettmann K., Potterat O., Iridoid glucosides with free radical scavenging properties from Fagraea blumei, Helvetica Chimica Acta, 80 (4) (1997) 1144-1152.
  • [19] Benzie I. F., Strain J. J., The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay, Analytical biochemistry., 239 (1) (1996) 70-76.
  • [20] Joseph J., Paul D., Kavitha M.P., Dineshkumar B., Menon J.S., Bhat A.R. and Krishnakumar K., Preliminary phytochemical screening and in vitro antioxidant activity of Banana flower (Musa paradisiaca AAB Nendran variety), Journal of Pharmacy Research, 8 (2) (2014) 144 – 1519.
  • [21] Chang C.C., Yang M.H., Wen H.M., Chern J.C., Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis, 10 (3) (2002) 178–182.
  • [22] Gezegen H., Tutar U., Hepokur C., Ceylan M., Synthesis and biological evaluation of novel indenopyrazole derivatives, J Biochem Mol Toxicol., 33(5) (2019) :e22285. doi: 10.1002/jbt.22285
  • [23] Kaur C., Dalamu M., Singh S., Walia S., Joshi A.D., Variations in phenolics and antioxidants in Indian onions (Allium cepa L.) Genotype selection for breeding, Nutr. Food Sci., 40 (1) (2004) 6–19
  • [24] Mzabri I., Addi M., Berrichi A., Traditional and Modern Uses of Saffron (Crocus Sativus), Cosmetics, 6 (4) (2019) 63.
  • [25] Somayeh R., Sohrab M., Maryam H., Seyed A.S., Evaluation of antioxidant activities of bioactive compounds and various extracts obtained from saffron (Crocus sativus L.): A review, J. Food Sci. Technol., 52 (2015) 1881–1888.
  • [26] Karimi E., Oskoueian E., Hendra R., Jaafar H.Z., Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity, Molecules, 15 (9) (2010) 6244–6256.
  • [27] Forni C., Facchiano F., Bartoli M., Beneficial Role of Phytochemicals on Oxidative Stress and Age-Related Diseases, BioMed Res. Int., 7 (2019) 1-16.
  • [28] Makhlouf H., Saksouk M., Habib J., Chahine R., Determination of antioxidant activity of saffron taken from the flower of Crocus sativus grown in Lebanon., African Journal of Biotechnology, 10 (41) (2011) 8093-8100.
  • [29] Hawa Z., Jaafari E., Bahtaei D., Evaluation of Crocus sativus L. Stigma Phenolic and Flavonoid Compounds and Its Antioxidant Activity, Molecules, 15 (9) (2010) 6244-6256.
  • [30] Tavakkol-Afshari J., Brook A., Mousavi S. H., Study of cytotoxic and apoptogenic properties of saffron extract in human cancer cell lines, Food and Chemical Toxicology, 46 (11) (2018) 3443-3447.
  • [31] Kondhare D., Lade H., Phytochemical profile, aldose reductase inhibitory, and antioxidant activities of indian traditional medicinal Coccinia grandis (L.) fruit extract, Biotech., 7 (6) (2017) 378.
  • [32] Wali A. F., Alchamat H. A. A., Hariri H. K., Hariri B. K., Menezes G. A., Zehra U., Ahmad, P., Antioxidant, antimicrobial, antidiabetic and cytotoxic activity of Crocus sativus L. Petals, Applied Sciences., 10 (4) (2020) 1519.
There are 32 citations in total.

Details

Primary Language English
Subjects Metabolic Medicine
Journal Section Natural Sciences
Authors

Sinan Soylu 0000-0002-3911-3227

Publication Date March 28, 2024
Submission Date January 14, 2024
Acceptance Date March 15, 2024
Published in Issue Year 2024Volume: 45 Issue: 1

Cite

APA Soylu, S. (2024). Crocus Officinalis (L.) Extract on Human Colerectal Cancer Cell Line (HT-22): Investigation in Vitro. Cumhuriyet Science Journal, 45(1), 35-39. https://doi.org/10.17776/csj.1419826
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