DNA Barcoding of Commercial Cockroaches in Turkey

Şeyda Berk; Ayşe Nur Pektaş

doi:10.17776/csj.1196804

EN

DNA Barcoding of Commercial Cockroaches in Turkey

Abstract

Accurate species identification has become a precondition for accomplished biodiversity administration and further genetic research. Species acquaintance technics require molecular tools such as DNA barcoding as well as morphological identification for accurate identification. Particularly, the application of subunit I of the mitochondrial cytochrome c oxidase (COI) gene for DNA barcoding for insects has approved to be very useful in species acquaintance. The main aim of this study is to generate the first reference library of DNA barcode for cockroaches in Turkey using previously published data. As a result of the literature research, it has been observed that no study has been carried out on the DNA barcode of Turkish cockroaches. Therefore, in this study, we evaluated the advantage of DNA barcoding applied to two cockroach samples from Turkey for the first time. Our working samples implicated 10 DNA barcodes grounded on sequences created from our present study and 109 other DNA barcodes from BOLD. Various molecular analyzes including genetic distance-origin assessment (NeighborJoining and Maximum Likelihood trees) has been applied to accurately identify and describe species. In addition, Blaptica dubia (B. dubia) (Serville, 1838) and Nauphoeta cinerea (N. cinerea) (Olivier, 1789) have been reported as the first country records. It has been observed that reference libraries like BOLD are not yet sufficiently populated with COI sequences of Turkish cockroach species. In order for Turkish cockroach bio-assessment and biodiversity studies to benefit from the advantages of DNA barcoding, it is of great importance that cockroach inventories and taxonomic studies include DNA barcodes.

Keywords

Thanks

All analyses in this study were performed in the Molecular Biology laboratory of the Sivas Cumhuriyet University Advanced Technology and Research Center (CUTAM).

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Şeyda Berk ^*
0000-0003-4687-0223
Türkiye

Ayşe Nur Pektaş
0000-0001-5621-2844
Türkiye

Publication Date

March 26, 2023

Submission Date

October 31, 2022

Acceptance Date

March 14, 2023

Published in Issue

Year 2023 Volume: 44 Number: 1

DOI

https://doi.org/10.17776/csj.1196804

IZ

https://izlik.org/JA93HD87CR

Cite

RIS / Bibtex

APA

Berk, Ş., & Pektaş, A. N. (2023). DNA Barcoding of Commercial Cockroaches in Turkey. Cumhuriyet Science Journal, 44(1), 28-35. https://doi.org/10.17776/csj.1196804

Öz

DNA Barcoding of Commercial Cockroaches in Turkey

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite