Activity Improvement and Thermal Stability Enhancement of D-Aminoacylase Using Protein-Polymer Conjugates

Sema Bilgin; Nazan Gökşen Tosun; Cemil Alkan; Esra Koç; Seçil Erden Tayhan

doi:10.17776/csj.1003429

EN TR

Activity Improvement and Thermal Stability Enhancement of D-Aminoacylase Using Protein-Polymer Conjugates

Abstract

In this study, the synthesis of new polymer-protein conjugates using a grafting-from strategy was performed by employing photo-induced electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. D-aminoacylase is an industrially significant enzyme for the preparation of chiral amino acids and it is coupled with reversible addition-fragmentation (RAFT) chain transfer agent (CTA) using activated ester chemistry. The effects of polymeric side chain compositions on the activity of D-aminoacylase were studied with two different polymeric side chain lengths. For this reason, two monomers, a hydrophilic N-(2-aminoethyl acrylamide) and a hydrophobic and N- (iso-butoxymethyl) acrylamide were used, respectively. It was found that modification by grafting from strategy increased the thermal stability of D-aminoacylase enzyme. Additionally, the hydrophobic monomer conjugate has been reported to increase the activity of the enzyme more than the hydrophilic monomer.

Keywords

Öz

Bu çalışmada, aşılama stratejisi kullanılarak yeni polimer-protein konjugatlarının sentezi foto-indüklenmiş elektron transferi tersinir ekleme-parçalanma zincir transferi (PET-RAFT) polimerizasyonu ile gerçekleştirilmiştir. D-aminoaçilaz, kiral amino asitlerin hazırlanmasında kullanılan endüstriyel olarak önemli bir enzimdir ve aktive edilmiş ester kimyası kullanılarak tersinir ekleme-parçalama (RAFT) zincir transfer maddesi (CTA) ile birleştirilir. Polimerik yan zincir bileşimlerinin D-aminoaçilaz aktivitesi üzerindeki etkileri, iki farklı polimerik yan zincir uzunluğu ile incelenmiştir. Bu nedenle sırasıyla hidrofilik N-(2-aminoetil akrilamid) ve hidrofobik ve N-(izo-bütoksimetil) akrilamid olmak üzere iki farklı monomer kullanılmıştır. D-aminoaçilaz enziminin aşılama stratejisi ile modifikasyonun, enziminin termal stabilitesini arttırdığı bulundu. Ek olarak, hidrofobik monomer konjugatının, enzimin aktivitesini hidrofilik monomerden daha fazla arttırdığı rapor edilmiştir.

Anahtar Kelimeler

Supporting Institution

Tokat Gaziosmanpaşa Üniversitesi

Project Number

2014/59

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Sema Bilgin
Türkiye

Nazan Gökşen Tosun ^*
0000-0001-5269-1067
Türkiye

Cemil Alkan
0000-0002-1509-4789
Türkiye

Esra Koç
0000-0001-7171-608X
Türkiye

Seçil Erden Tayhan
0000-0001-8473-5896
Türkiye

Publication Date

December 27, 2022

Submission Date

October 1, 2021

Acceptance Date

October 2, 2022

Published in Issue

Year 2022 Volume: 43 Number: 4

DOI

https://doi.org/10.17776/csj.1003429

IZ

https://izlik.org/JA55TN45AF

Cite

RIS / Bibtex

APA

Bilgin, S., Gökşen Tosun, N., Alkan, C., Koç, E., & Erden Tayhan, S. (2022). Activity Improvement and Thermal Stability Enhancement of D-Aminoacylase Using Protein-Polymer Conjugates. Cumhuriyet Science Journal, 43(4), 621-628. https://doi.org/10.17776/csj.1003429