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An Investigation of the Bactericidal and Fungicidal Effects of Algerian Propolis Extracts and Essential Oils

Yıl 2022, Cilt 43, Sayı 1, 14 - 19, 30.03.2022
https://doi.org/10.17776/csj.936040

Öz

Natural products have been used in medicine for variety of purposes for centuries. As a natural product, propolis is gaining increasing importance today due to its antimicrobial activity against pathogenic microorganisms. Hence, the aim of the present study was to investigate the antimicrobial activity of extracts and essential oils of propolis collected from various regions of Algeria against food-borne and clinically pathogen microorganisms including Salmonella enteritidis RSKK 171, Shigella sonnei MU:57 and Candida glabrata RSKK 04019. The antimicrobial activity of the propolis extracts and essential oils were evaluated using disc diffusion method. The results showed that all propolis extracts and essential oils exhibited antimicrobial activity against the tested microorganisms with inhibition zones varied from 8.31 mm to 14.53 mm. The minimal inhibitory concentration (MIC) and minimal bactericidal or fungicidal concentration (MBC or MFC) of the samples were determined by microdilution-broth method. The MIC and MBC or MFC values were in the range of 0.25-2 µg/µl and 0.25-4 µg/µl. Therefore, propolis extracts and essential oils from different regions of Algeria have potential to be used as a natural additive for food and pharmaceutical industries.

Kaynakça

  • [1] Tajkarimi M.M., Ibrahim S.A., Cliver D.O., Antimicrobial herb and spice compounds in food, Food Control, 21 (2010) 1199–1218.
  • [2] Karatepe P., Patir B., The effect of eugenol and thymol on chemical, microbiological and sensory quality of pasteurized butter, Firat University Medical Journal of Health Sciences, 26 (2012) 35-46.
  • [3] Oskay M., Tamer A.U., Ay G., Sari D., Akta K., Antimicrobial activity of the leaves of Lippia triphylla (L'Her) O. Kuntze (Verbenaceae) against on bacteria and yeasts, J. Biol. Sci., 5(5) (2005) 620-622.
  • [4] da Silva C., Prasniewski A., Calegari M.A., de Lima V.A., Oldoni T.L., Determination of total phenolic compounds and antioxidant activity of ethanolic samples of propolis using ATR–FT-IR spectroscopy and chemometrics, Food Anal. Methods, 11(7) (2018) 2013-2021.
  • [5] Segueni N., Khadraoui F., Rhouati S., Volatile compounds as propolis characterization markers. In Euro-Mediterranean Conference for Environmental Integration, November, Springer, (2017) 1271-1273.
  • [6] Ghisalberti E.L., Propolis: a review, Bee World, 60 (1979) 59–84.
  • [7] El-Ghamdi A.A., Bayaqoob N.I., Rushdi A.I., Alattal Y., Simoneit B.R., El-Mubarak A.H., Al-Mutlaq K.F., Chemical compositions and characteristics of organic compounds in propolis from Yemen, Saudi J. Biol. Sci., 24(5) (2017) 1094-1103.
  • [8] Kumazawa S., Hamasaka T., Nakayama T., Antioxidant activity of propolis of various geographic origins, Food Chem., 84 (2004) 329–339.
  • [9] Yadav H., Mungara P., Jivrajani M., Nivsarkar M., Anandjiwala S., TLC-densitometric quantification of negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol using HPTLC from vitex negundo leaves, J. Liq. Chromatogr. Relat. Technol., 35(11) (2012) 1565-1584.
  • [10] Piccinelli A.L., Mencherini T., Celano R., Mouhoubi Z., Tamendjari A., Aquino R.P., Rastrelli L., Chemical composition and antioxidant activity of Algerian propolis, J. Agric. Food Chem., 61(21) (2013) 5080-5088.
  • [11] de Moura S.A.L., Negri G., Salatino A., Lima L.D.C., Dourado L.P.A., Mendes J.B., Aqueous sample Brazilian propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges, Evid. Based Complement. Alternat. Med., 18 (2009) 1–9.
  • [12] Wang R., Ding S., Zhao D., Wang Z., Wu J., Hu W., Effect of dehydration methods on antioxidant activities, phenolic contents, cyclic nucleotides, and volatiles of jujube fruits, Food Sci. Biotechnol., 25(1) (2016) 137-143.
  • [13] Kang L.J., Lee H.B., Bae H.J., Lee S.G., Antidiabetic effect of propolis: reduction of expression of glucose‐6‐phosphatase through inhibition of Y279 and Y216 autophosphorylation of GSK‐3α/β in HepG2 cells. Phytother. Res., 24(10) (2010) 1554-1561.
  • [14] de Castro P.A., Savoldi M., Bonatto D., Malavazi I., Goldman M.H., Berretta A.A., Transcriptional profiling of Saccharomyces cerevisiae exposed to propolis, BMC Complement. Altern. Med., 12 (2012) 194.
  • [15] Silva F., Domingues F.C., Antimicrobial activity of coriander oil and its effectiveness as food preservative, Crit. Rev. Food Sci. Nutr., 57 (2017) 35-47.
  • [16] Martins N., Ferreira I.C.F.R., Barros L., Silva S., Henriques M., Candidiasis: predisposing factors, prevention, diagnosis and alternative treatment, Mycopathologia, 177(5–6) (2014) 223-240
  • [17] Douglas LJ., Candida biofilms and their role in infection, Trends Microbiol., 11(1) (2003) 30-36.
  • [18] Wu F.M., Doyle M.P., Beuchat L.A., Wells J.G., Mintz E.D., Swaminathan B. Fate of Shigella sonnei on parsley and methods of disinfection, J. Food Prot., 63 (2000) 568-572.
  • [19] Lampel, K.A., Maurelli, A.T., 2002. Shigella. In: Cliver, D.O., Riemann, H.P. (Eds.), Foodborne Diseases. 2nd ed. London: Elsevier Science Ltd, ( 2002) 69-77.
  • [20] Gouws P.A., Visser M., Brözel V.S., A polymerase chain reaction procedure for the detection of Salmonella sp. within 24 hours, J. Food Prot. 61(8) (1998) 1039-1042.
  • [21] Akpınar M., Salmonella ile bakteriyofajlarının çeşitli köy kümes folluklarından izolasyonu ve karakterizasyonu. Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Ankara Üniversitesi, Ankara (2020).
  • [22] Sforcin J.M., Fernandes Jr., Lopes C.A.M., Bankova V., Funari S.R.C., Seasonal effect on Brazilian propolis antibacterial activity, J. Ethnopharmacol., 73 (2000) 243–249.
  • [23] Hegazi A.G., Abd El Hady F.K., Abd Allah F.A., Chemical composition and antimicrobial activity of European propolis, Z. Naturforsch. C, 55 (2000) 70–75.
  • [24] Park Y.K., Ikegaki M., Preparation of water and ethanolic extracts of propolis and evaluation of the preparations, Bioscience, Biotechnology and Biochemistry, 62(11) (1998) 2230–2232.
  • [25] Murray P.R., Baron E.J., Pfaller M.A., Tenevor F.C., Yolke R.H., Manual of Clinical Microbiology. 6th ed. Washington: ASM Press, (1995) 457–464.
  • [26] Kujumgiev A., Bankova V., Ignatova A., Popov S., Antibacterial activity of propolis, some of its components and analogs, Pharmazie, 48 (1993) 785-786.
  • [27] Krishnan N., Ramanathan S., Sasidharan S., Murugaiyah V., Mansor S.M., Antimicrobial activity evaluation of Cassia spectabilis leaf samples, Int. J. Pharmacol., 6(4) (2010) 510–514.
  • [28] Hazen K.C., Fungicidal versus fungistatic activity of terbinafine and itraconazole: An in vitro comparison, J. Am. Acad. Dermatol., 38(5) (1998) 37-41.
  • [29] Marcucci M.C., Propolis: Chemical composition, biological properties and therapeutic activity, Apidologie, 26 (1995) 83-99.
  • [30] De Castro S.L., Higashi K.O., Effect of different formulations of propolis on mice infected with Trypanosoma cruzi, J. Ethnopharmacol., 46 (1995) 55–58.
  • [31] Bhavaniramya S., Vishnupriya S., Al-Aboody M.S., Vijayakumar R., Baskaran D., Role of essential oils in food safety: Antimicrobial and antioxidant applications, Grain & Oil Science and Technology, 2(2) (2019) 49-55.
  • [32] Nieva M.M.I., Isla M.I., Cudmani N.G., Vattuone M.A., Sampietro A.R., Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis, J. Ethnopharmacol., 68 (1999) 97–102.
  • [33] Grange J.M., Davey R.W., Antibacterial properties of propolis (bee glue), Journal of the Royal Society of Medicine, 83 (1990) 159–161.
  • [34] Przybyłek I., Karpinski T.M. Antibacterial properties of propolis, Molecules, 24(11) (2019) 2047.
  • [35] Al-An I., Zimmermann S., Reichling J., Wink M., Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics, Medicines, (1) (2018) 2.
  • [36] Gür N., Bayrak N., Topdemir A., Determination of antimicrobial activity and some biochemical properties of honey and propolis in Turkish markets, Prog. Nutr., 22(3) (2020) e2020040.
  • [37] Mohdaly A.A.A., Mahmoud A.A., Roby M.H.H., Smetanska I., Ramadan M.F., Phenolic extract from propolis and bee pollen: composition, antioxidant and antibacterial activities, J. Food Biochem., 39 (2015) 538–547.
  • [38] Bouzahouane H., Ayari A., Guehria I., Riah O., Propolis: Antimicrobial activity and chemical composition analysis: Properties of propolis, J. Microbiol. Biotech. Food Sci., 10(6) (2021) e3211.
  • [39] Seidel V., Peyfoon E., Watson D.G., Fearnley J., Comparative study of the antibacterial activity of propolis from different geographical and climatic zones, Phytother. Res., 22(2008) 1256–1263.

Yıl 2022, Cilt 43, Sayı 1, 14 - 19, 30.03.2022
https://doi.org/10.17776/csj.936040

Öz

Kaynakça

  • [1] Tajkarimi M.M., Ibrahim S.A., Cliver D.O., Antimicrobial herb and spice compounds in food, Food Control, 21 (2010) 1199–1218.
  • [2] Karatepe P., Patir B., The effect of eugenol and thymol on chemical, microbiological and sensory quality of pasteurized butter, Firat University Medical Journal of Health Sciences, 26 (2012) 35-46.
  • [3] Oskay M., Tamer A.U., Ay G., Sari D., Akta K., Antimicrobial activity of the leaves of Lippia triphylla (L'Her) O. Kuntze (Verbenaceae) against on bacteria and yeasts, J. Biol. Sci., 5(5) (2005) 620-622.
  • [4] da Silva C., Prasniewski A., Calegari M.A., de Lima V.A., Oldoni T.L., Determination of total phenolic compounds and antioxidant activity of ethanolic samples of propolis using ATR–FT-IR spectroscopy and chemometrics, Food Anal. Methods, 11(7) (2018) 2013-2021.
  • [5] Segueni N., Khadraoui F., Rhouati S., Volatile compounds as propolis characterization markers. In Euro-Mediterranean Conference for Environmental Integration, November, Springer, (2017) 1271-1273.
  • [6] Ghisalberti E.L., Propolis: a review, Bee World, 60 (1979) 59–84.
  • [7] El-Ghamdi A.A., Bayaqoob N.I., Rushdi A.I., Alattal Y., Simoneit B.R., El-Mubarak A.H., Al-Mutlaq K.F., Chemical compositions and characteristics of organic compounds in propolis from Yemen, Saudi J. Biol. Sci., 24(5) (2017) 1094-1103.
  • [8] Kumazawa S., Hamasaka T., Nakayama T., Antioxidant activity of propolis of various geographic origins, Food Chem., 84 (2004) 329–339.
  • [9] Yadav H., Mungara P., Jivrajani M., Nivsarkar M., Anandjiwala S., TLC-densitometric quantification of negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol using HPTLC from vitex negundo leaves, J. Liq. Chromatogr. Relat. Technol., 35(11) (2012) 1565-1584.
  • [10] Piccinelli A.L., Mencherini T., Celano R., Mouhoubi Z., Tamendjari A., Aquino R.P., Rastrelli L., Chemical composition and antioxidant activity of Algerian propolis, J. Agric. Food Chem., 61(21) (2013) 5080-5088.
  • [11] de Moura S.A.L., Negri G., Salatino A., Lima L.D.C., Dourado L.P.A., Mendes J.B., Aqueous sample Brazilian propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges, Evid. Based Complement. Alternat. Med., 18 (2009) 1–9.
  • [12] Wang R., Ding S., Zhao D., Wang Z., Wu J., Hu W., Effect of dehydration methods on antioxidant activities, phenolic contents, cyclic nucleotides, and volatiles of jujube fruits, Food Sci. Biotechnol., 25(1) (2016) 137-143.
  • [13] Kang L.J., Lee H.B., Bae H.J., Lee S.G., Antidiabetic effect of propolis: reduction of expression of glucose‐6‐phosphatase through inhibition of Y279 and Y216 autophosphorylation of GSK‐3α/β in HepG2 cells. Phytother. Res., 24(10) (2010) 1554-1561.
  • [14] de Castro P.A., Savoldi M., Bonatto D., Malavazi I., Goldman M.H., Berretta A.A., Transcriptional profiling of Saccharomyces cerevisiae exposed to propolis, BMC Complement. Altern. Med., 12 (2012) 194.
  • [15] Silva F., Domingues F.C., Antimicrobial activity of coriander oil and its effectiveness as food preservative, Crit. Rev. Food Sci. Nutr., 57 (2017) 35-47.
  • [16] Martins N., Ferreira I.C.F.R., Barros L., Silva S., Henriques M., Candidiasis: predisposing factors, prevention, diagnosis and alternative treatment, Mycopathologia, 177(5–6) (2014) 223-240
  • [17] Douglas LJ., Candida biofilms and their role in infection, Trends Microbiol., 11(1) (2003) 30-36.
  • [18] Wu F.M., Doyle M.P., Beuchat L.A., Wells J.G., Mintz E.D., Swaminathan B. Fate of Shigella sonnei on parsley and methods of disinfection, J. Food Prot., 63 (2000) 568-572.
  • [19] Lampel, K.A., Maurelli, A.T., 2002. Shigella. In: Cliver, D.O., Riemann, H.P. (Eds.), Foodborne Diseases. 2nd ed. London: Elsevier Science Ltd, ( 2002) 69-77.
  • [20] Gouws P.A., Visser M., Brözel V.S., A polymerase chain reaction procedure for the detection of Salmonella sp. within 24 hours, J. Food Prot. 61(8) (1998) 1039-1042.
  • [21] Akpınar M., Salmonella ile bakteriyofajlarının çeşitli köy kümes folluklarından izolasyonu ve karakterizasyonu. Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Ankara Üniversitesi, Ankara (2020).
  • [22] Sforcin J.M., Fernandes Jr., Lopes C.A.M., Bankova V., Funari S.R.C., Seasonal effect on Brazilian propolis antibacterial activity, J. Ethnopharmacol., 73 (2000) 243–249.
  • [23] Hegazi A.G., Abd El Hady F.K., Abd Allah F.A., Chemical composition and antimicrobial activity of European propolis, Z. Naturforsch. C, 55 (2000) 70–75.
  • [24] Park Y.K., Ikegaki M., Preparation of water and ethanolic extracts of propolis and evaluation of the preparations, Bioscience, Biotechnology and Biochemistry, 62(11) (1998) 2230–2232.
  • [25] Murray P.R., Baron E.J., Pfaller M.A., Tenevor F.C., Yolke R.H., Manual of Clinical Microbiology. 6th ed. Washington: ASM Press, (1995) 457–464.
  • [26] Kujumgiev A., Bankova V., Ignatova A., Popov S., Antibacterial activity of propolis, some of its components and analogs, Pharmazie, 48 (1993) 785-786.
  • [27] Krishnan N., Ramanathan S., Sasidharan S., Murugaiyah V., Mansor S.M., Antimicrobial activity evaluation of Cassia spectabilis leaf samples, Int. J. Pharmacol., 6(4) (2010) 510–514.
  • [28] Hazen K.C., Fungicidal versus fungistatic activity of terbinafine and itraconazole: An in vitro comparison, J. Am. Acad. Dermatol., 38(5) (1998) 37-41.
  • [29] Marcucci M.C., Propolis: Chemical composition, biological properties and therapeutic activity, Apidologie, 26 (1995) 83-99.
  • [30] De Castro S.L., Higashi K.O., Effect of different formulations of propolis on mice infected with Trypanosoma cruzi, J. Ethnopharmacol., 46 (1995) 55–58.
  • [31] Bhavaniramya S., Vishnupriya S., Al-Aboody M.S., Vijayakumar R., Baskaran D., Role of essential oils in food safety: Antimicrobial and antioxidant applications, Grain & Oil Science and Technology, 2(2) (2019) 49-55.
  • [32] Nieva M.M.I., Isla M.I., Cudmani N.G., Vattuone M.A., Sampietro A.R., Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis, J. Ethnopharmacol., 68 (1999) 97–102.
  • [33] Grange J.M., Davey R.W., Antibacterial properties of propolis (bee glue), Journal of the Royal Society of Medicine, 83 (1990) 159–161.
  • [34] Przybyłek I., Karpinski T.M. Antibacterial properties of propolis, Molecules, 24(11) (2019) 2047.
  • [35] Al-An I., Zimmermann S., Reichling J., Wink M., Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics, Medicines, (1) (2018) 2.
  • [36] Gür N., Bayrak N., Topdemir A., Determination of antimicrobial activity and some biochemical properties of honey and propolis in Turkish markets, Prog. Nutr., 22(3) (2020) e2020040.
  • [37] Mohdaly A.A.A., Mahmoud A.A., Roby M.H.H., Smetanska I., Ramadan M.F., Phenolic extract from propolis and bee pollen: composition, antioxidant and antibacterial activities, J. Food Biochem., 39 (2015) 538–547.
  • [38] Bouzahouane H., Ayari A., Guehria I., Riah O., Propolis: Antimicrobial activity and chemical composition analysis: Properties of propolis, J. Microbiol. Biotech. Food Sci., 10(6) (2021) e3211.
  • [39] Seidel V., Peyfoon E., Watson D.G., Fearnley J., Comparative study of the antibacterial activity of propolis from different geographical and climatic zones, Phytother. Res., 22(2008) 1256–1263.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Bölüm Natural Sciences
Yazarlar

Meltem AŞAN ÖZÜSAĞLAM (Sorumlu Yazar)
AKSARAY UNIVERSITY
0000-0002-3638-1306
Türkiye


Songül TACER Bu kişi benim
AKSARAY UNIVERSITY
0000-0002-7035-8134
Türkiye


Safia BOULECHFAR Bu kişi benim
University of Larbi Ben Mhidi Oum El Bouaghi
0000-0002-2716-0261
Algeria


Amar ZELLAGUI Bu kişi benim
University of Larbi Ben Mhidi Oum El Bouaghi
0000-0002-6515-8103
Algeria

Yayımlanma Tarihi 30 Mart 2022
Başvuru Tarihi 21 Mayıs 2021
Kabul Tarihi 27 Ocak 2022
Yayınlandığı Sayı Yıl 2022, Cilt 43, Sayı 1

Kaynak Göster

APA Aşan Özüsağlam, M. , Tacer, S. , Boulechfar, S. & Zellaguı, A. (2022). An Investigation of the Bactericidal and Fungicidal Effects of Algerian Propolis Extracts and Essential Oils . Cumhuriyet Science Journal , 43 (1) , 14-19 . DOI: 10.17776/csj.936040