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fCNT ve Oksidasyonun OGG/fCNT Çift Ağlı Hidrojel Üzerindeki Etkileri

Year 2021, Volume: 42 Issue: 4, 822 - 829, 29.12.2021

Serbülent Türk

Abstract

Fonksiyonelleştirilmiş karbon nanotüp (fCNT) ve oksitlenmiş jellan zamkından (OGG) oluşan çift ağlı (DN) hidrojel sistemleri, iyonik çapraz bağlama ile başarılı bir şekilde geliştirilmiştir. Bu çalışmada geliştirilmiş modül ve jelasyon sıcaklığına (Tgelasyon) sahip bir DN hibrit hidrojel elde etmek için OGG sentezlendi ve fCNT ile karıştırıldı. Hazırlanan OGG-fCNT hidrojellerinin jelleşme ve fiziko-kimyasal özellikleri üzerine hidrojen peroksit (H2O2) oksidasyonu ve fCNT ilavesinin etkileri araştırıldı. OGG ve hazırlanan OGG-fCNT DN hidrojelleri, OGG-fCNTs DN hidrojellerinin fizikokimyasal özellikleri üzerindeki H2O2 oksidasyonunu ve CNT etkileri SEM, FTIR, Zeta potansiyeli, temas açısı ve Tgelation ölçümleri ile karakterize edildi. Oksidasyon sonrası Tgelasyon derecesi fizyolojik sıcaklığın altına başarılı bir şekilde düşürülürken, bu sıcaklık fCNT ile karıştırılarak daha da düşürüldü. Sentezlenen DN hidrojeli, fCNT ile 1389 Pa'dan 4895 Pa'ya artan bir modül gösterdi. OGG-fCNT DN hidrojel sisteminin morfolojik yapısı, fCNT ilavesinden önemli ölçüde etkilenmiştir. OGG-fCNT hidrojel sistemi için yapılan bu çalışma, bileşenler arasındaki etkileşimi ve üç boyutlu bir GG yapısının özelliklerinin oksidasyon ve fCNT varlığından etkilenebileceğini göstermiştir. Bu çalışmadan elde edilen gözlemlerin, GG'nin doku mühendisliğinde bir biyomateryal ve taşıyıcı sistemlerde ilaç taşıyıcı olarak uygulanması için gerekli olabileceği anlaşılmıştır.

Keywords

Gellan zamkı Oksidasyon Reolojik analiz. Çİft ağlı hidrojel

Project Number

2019-6-23-223

References

  • [1] Mondal S., Das S., Nandi A.K. , A review on recent advances in polymer and peptide hydrogels, Soft Matter, 16 (2020) 1404–1454.
  • [2] Dorishetty P., Dutta N.K., Choudhury N.R., Bioprintable tough hydrogels for tissue engineering applications, Advances in Colloid and Interface Science, 281(102163) (2020) 1-23.
  • [3] Yang J., Zhang Y.S., Yue K., Khademhosseini A. , Cell-laden hydrogels for osteochondral and cartilage tissue engineering, Bone Research, 5(17014) (2017) 1–20.
  • [4] Bonifacio M.A., Cometa S., Cochis A., Gentile P., Ferreira A.M., Azzimonti B., Procino G., Ceci E., Rimondini L., De Giglio E. , Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair, Carbohydrate Polymers, 198 (2018) 462–472.
  • [5] Kouhi M., Varshosaz J., Hashemibeni B., Sarmadi A., Injectable gellan gum / lignocellulose nano fi brils hydrogels enriched with melatonin loaded forsterite nanoparticles for cartilage tissue engineering : Fabrication, characterization and cell culture studies, Materials Science & Engineering C, 115(111114) (2020) 1–12.
  • [6] Palumbo F.S., Federico S., Pitarresi G., Fiorica C., Giammona G. , Gellan gum-based delivery systems of therapeutic agents and cells, Carbohydrate Polymers, 229(115430) (2020) 1-19.
  • [7] Liu S., Qiu Y., Yu W., Zhang H. , Highly Stretchable and Self-Healing Strain Sensor Based on Gellan Gum Hybrid Hydrogel for Human Motion Monitoring, ACS Applied Polymer Materials, 2(3) (2020) 1325–1334.
  • [8] Zhang X., Pan Y., Li S., Xing L., Du S., Yuan G., Li J., Zhou T., Xiong D., Tan H., Ling Z., Chen Y., Hu X., Niu X. , Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing, International Journal of Biological Macromolecules, 164 (2020) 2204–2214.
  • [9] Osmałek T.Z., Froelich A., Jadach B. , Rheological investigation of high-acyl gellan gum hydrogel and its mixtures with simulated body fluids, Journal of Biomaterials Applications, 32(10) (2018) 1435–1449.
  • [10] Mohammadinejad R., Kumar A., Ranjbar-Mohammadi M., Ashrafizadeh M., Han S.S., Khang G., Roveimiab Z. , Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review, Polymers, 12(176) (2020) 1-38.
  • [11] Wang D., Li C., Gong Y., Wang C., Lai R.C., Su K., Zhang F., Wang D. , An improved injectable polysaccharide hydrogel : modified gellan gum for long-term cartilage regeneration in vitro, Journal of Materials Chemistry, 19(14) (2009) 1968–1977.
  • [12] Türk S., Altınsoy I., Çelebi Efe G., Ipek M., Özacar M., Bindal C. , 3D porous collagen/functionalized multiwalled carbon nanotube/chitosan/hydroxyapatite composite scaffolds for bone tissue engineering, Materials Science & Engineering C, 92 (2018) 757–768.
  • [13] Lu Y., Zhao X., Fang S. , Characterization, Antimicrobial Properties and Coatings Application of Gellan Gum Oxidized with Hydrogen Peroxide, Foods, 8(31) (2019) 1–12.
  • [14] Wang P., Luo Z., Xiao Z. , Preparation, physicochemical characterization and in vitro release behavior of resveratrol-loaded oxidized gellan gum/resistant starch hydrogel beads, Carbohydrate Polymers, 260(117794) (2021) 1-10.

The effects of fCNT and oxidation on the OGG/fCNT double networked hydrogel

Year 2021, Volume: 42 Issue: 4, 822 - 829, 29.12.2021

Serbülent Türk

Abstract

Double networked (DN) hydrogel systems consisting of fCNT and OGG have been successfully developed by ionic crosslinking. OGG was synthesized and mixed with fCNT to obtain DN hybrid hydrogel with improved modulus and Tgelation. The effects of H2O2 oxidation and fCNT addition on the gelling and physico-chemical features of the prepared OGG-fCNT hydrogels were investigated. The OGG and prepared OGG-fCNT DN hydrogels were characterized by SEM, FTIR, zeta potential, contact angle, and Tgelation measurements to evaluate H2O2 oxidation and CNT effects on the physicochemical properties. While the degree of Tgelation after oxidation is successfully reduced below physiological temperature, this temperature was further lowered by mixing with fCNT. The synthesized DN hydrogel showed an increasing modulus from 1389 Pa to 4895 Pa with fCNT. The morphological structure of the OGG-fCNT DN hydrogel system was significantly affected by the addition of fCNT. This study for the OGG-fCNT hydrogel system demonstrated the interaction between components and the properties of a three-dimensional GG structure can be affected by oxidation and the presence of fCNT. The obtained observations from this study may be necessary for the application of GG as a biomaterial in tissue engineering and drug carrier in delivery systems.

Keywords

Double networked hydrogel Gellan gum Oxidation Rheological analysis.

Supporting Institution

Sakarya Üniversitesi

Project Number

2019-6-23-223

Thanks

This work was supported by the Scientific Research Projects Commission of Sakarya University (Project number: 2019-6-23-223). The author thanks Dr. Muaz Kemerli for assisting with rheological studies.

References

  • [1] Mondal S., Das S., Nandi A.K. , A review on recent advances in polymer and peptide hydrogels, Soft Matter, 16 (2020) 1404–1454.
  • [2] Dorishetty P., Dutta N.K., Choudhury N.R., Bioprintable tough hydrogels for tissue engineering applications, Advances in Colloid and Interface Science, 281(102163) (2020) 1-23.
  • [3] Yang J., Zhang Y.S., Yue K., Khademhosseini A. , Cell-laden hydrogels for osteochondral and cartilage tissue engineering, Bone Research, 5(17014) (2017) 1–20.
  • [4] Bonifacio M.A., Cometa S., Cochis A., Gentile P., Ferreira A.M., Azzimonti B., Procino G., Ceci E., Rimondini L., De Giglio E. , Antibacterial effectiveness meets improved mechanical properties: Manuka honey/gellan gum composite hydrogels for cartilage repair, Carbohydrate Polymers, 198 (2018) 462–472.
  • [5] Kouhi M., Varshosaz J., Hashemibeni B., Sarmadi A., Injectable gellan gum / lignocellulose nano fi brils hydrogels enriched with melatonin loaded forsterite nanoparticles for cartilage tissue engineering : Fabrication, characterization and cell culture studies, Materials Science & Engineering C, 115(111114) (2020) 1–12.
  • [6] Palumbo F.S., Federico S., Pitarresi G., Fiorica C., Giammona G. , Gellan gum-based delivery systems of therapeutic agents and cells, Carbohydrate Polymers, 229(115430) (2020) 1-19.
  • [7] Liu S., Qiu Y., Yu W., Zhang H. , Highly Stretchable and Self-Healing Strain Sensor Based on Gellan Gum Hybrid Hydrogel for Human Motion Monitoring, ACS Applied Polymer Materials, 2(3) (2020) 1325–1334.
  • [8] Zhang X., Pan Y., Li S., Xing L., Du S., Yuan G., Li J., Zhou T., Xiong D., Tan H., Ling Z., Chen Y., Hu X., Niu X. , Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing, International Journal of Biological Macromolecules, 164 (2020) 2204–2214.
  • [9] Osmałek T.Z., Froelich A., Jadach B. , Rheological investigation of high-acyl gellan gum hydrogel and its mixtures with simulated body fluids, Journal of Biomaterials Applications, 32(10) (2018) 1435–1449.
  • [10] Mohammadinejad R., Kumar A., Ranjbar-Mohammadi M., Ashrafizadeh M., Han S.S., Khang G., Roveimiab Z. , Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review, Polymers, 12(176) (2020) 1-38.
  • [11] Wang D., Li C., Gong Y., Wang C., Lai R.C., Su K., Zhang F., Wang D. , An improved injectable polysaccharide hydrogel : modified gellan gum for long-term cartilage regeneration in vitro, Journal of Materials Chemistry, 19(14) (2009) 1968–1977.
  • [12] Türk S., Altınsoy I., Çelebi Efe G., Ipek M., Özacar M., Bindal C. , 3D porous collagen/functionalized multiwalled carbon nanotube/chitosan/hydroxyapatite composite scaffolds for bone tissue engineering, Materials Science & Engineering C, 92 (2018) 757–768.
  • [13] Lu Y., Zhao X., Fang S. , Characterization, Antimicrobial Properties and Coatings Application of Gellan Gum Oxidized with Hydrogen Peroxide, Foods, 8(31) (2019) 1–12.
  • [14] Wang P., Luo Z., Xiao Z. , Preparation, physicochemical characterization and in vitro release behavior of resveratrol-loaded oxidized gellan gum/resistant starch hydrogel beads, Carbohydrate Polymers, 260(117794) (2021) 1-10.
There are 14 citations in total.

Details

Primary Language English
Journal Section Natural Sciences
Authors

Serbülent Türk 0000-0003-4284-5397

Project Number 2019-6-23-223
Publication Date December 29, 2021
Submission Date July 2, 2021
Acceptance Date October 27, 2021
Published in Issue Year 2021Volume: 42 Issue: 4

Cite

APA Türk, S. (2021). The effects of fCNT and oxidation on the OGG/fCNT double networked hydrogel. Cumhuriyet Science Journal, 42(4), 822-829.