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Year 2020, Volume: 41 Issue: 4, 784 - 801, 29.12.2020

Adewole Sebiomo , Folake Banjo

https://doi.org/10.17776/csj.774034

Abstract

References

  • [1] Zabaloy M.C., Garland J.L. and Gómez M.A., An integrated approach to evaluate the impacts of the herbicides glyphosate, 2,4-D and metsulfuron-methyl on soil microbial communities in the Pampas region, Argentina, Appl. Soil Ecol., 40 (2008) 1–12.
  • [2] Pampulha M.E. and Oliveira A., Impact of an herbicide combination of bromoxynil and prosulfuron on soil microorganisms. Curr. Microbiol., 53 (2006) 238–243.
  • [3] Teng Y., Luo Y.M., Sun X.H., Tu C., Xu, L., and Liu W.X. Influence of arbuscular mycorrhiza and Rhizobium on phytoremediation by alfalfa of an agricultural soil contaminated with weathered PCBs: A field study, International Journal of Phytoremediation 12 (5) (2010) 516-533.
  • [4] Zhang J. and Chiao C., Novel approaches for remediation of pesticide pollutants, International Journal Environment and Pollution, 18 (5) (2002) 423-433.
  • [5] Kearney P. and Wauchope R., Disposal options based on properties of pesticides in soil and water, in: P. Kearney, T. Roberts (Eds.), Pesticideremediation in Soils and Water, Wiley Series in Agrochemicals and Plant Protection, (1998).
  • [6] Schoefs O., Perrier M. and Samson R., Estimation of contaminant depletion in unsaturated soils using a reduced-order biodegradation model and carbon dioxide measurement. App., Microbiol. Biotechnol., 64 (2004) 256-261.
  • [7] Pointing S.B., Feasibility of bioremediation by white-rot fungi. Appl. Microbiol. Biotechnol., 57 (2001) 20-33.
  • [8] Evans C. and Hedger J., Degradation of cell wall polymers. In: Fungi in bioremediation, Cambridge;. Gadd G. Ed Cambridge University Press., U.K.,2001.
  • [9] Sasek V., Why mycoremediations have not yet come to practice. In Sasek V. et al., (Eds.) In: The utilization of bioremediation to reduce soil contamination: Problems and solutions, Kluwer Academis Publishers. 2003; 247-276.
  • [10] Johal G.S. and Huber D.M,. Glyphosate effects on diseases of plants, Eur. J. Agron., 31 (2009) 144-152.
  • [11] Mbanaso F.U., Coupe S.J., Charlesworth S.M. and Nnadi E.O., Laboratory-based experiments to investigate the impact of glyphosate-containing herbicide on pollution attenuation and biodegradation in a model pervious paving system, Chemosphere, 90 (2013) 737-746.
  • [12] Shushkova T., Ermakova I. and Leontievsky A., Glyphosate bioavailability in soil, Biodegradation, 21 (2010) 403-410.
  • [13] Solomon K.R., Anadón A., Carrasquilla G. and Cerdeira A.L., Coca and poppy eradication in Colombia: environmental and human health assessment of aerially applied glyphosate, Rev. Environ. Contam. Toxicol., 190 (2007) 43-125.
  • [14] Leckie S.E., Methods of microbial community profiling and their application to forest soils, Forest Ecol. Manag., 220 (2005) 88-106.
  • [15] Kryuchkova Y.V., Burygin G.L., Gogoleva N.E. and Gogolev Y.V., Isolation and characterization of a glyphosate-degrading rhizosphere strain, Enterobacter cloacae K7. Microbiol. Res., 169 (2014) 99-105.
  • [16] Qiao X., Ma, L., and Hummel H.E. Persistence of atrazine and occurrence of its primary metabolites in three soils, Journal of Agriculture and Food Chemistry, 14 (1996) 187-192.
  • [17] Kruger E.L, Rice P.J., Anhalt J.C., Anderson T.A. and Coats, J.R. Use ofundisturbed soil columns under controlled conditions to study the fate of [14C] diethylatrazine, Journal of Agriculture and Food Chemistry, 44 (1996) 1144-1149.
  • [18] Ayansina A.D.V. and Oso B.A., Effect of two commonly used herbicides on soil microflora at two different concentrations, African Journal of Biotechnology, 5 (2) (2006) 129-4132.
  • [19] Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T. and Williams S.T., Bergye’s Manual of Determinative Bacteriology, 9th Ed. Baltimore, Maryland: Williams and Wilkins, 1994.
  • [20] Cheesbrough M., Medical Laboratory Manual for Tropical Countries, Microbiology. Linacre House, Jordan Hill, Oxford. Vol.11., (1984).
  • [21] Moneke A.N., Okpala G.N. and Anyanwu C.U., Biodegradation of glyphosate herbicide in vitro using bacterial isolates from four rice fields, African Journal of Biotechnology, 9 (26) (2010) 4067-4074.
  • [22] Diez M.C., Biological aspects involved in the degradation of organic pollutants, J. Soil. Sci. Plant Nutrition, 10(3) (2010) 244-267.
  • [23] Chirnside A., Ritter W. and Radosevich M., Isolation of selected microbial consortium from a pesticide-contaminated mixed load site soil capable of degrading the herbicides atrazine and alachlor, Soil Biology and Biochemistry, 39 (2007) 3056-3065.
  • [24] Whitelaw-Weckert M., Hutton R., Rouse E. and Lamont R., The effect of herbicides on permanent swards on soil microbial populations, In 3rd Australian New Zealand Soils Conference, University of Sydney, Australia, (2004) 1-5.
  • [25] Filimon M.N., Popescu R., Borozan A.B., Bordean D.M., Dumitrescu G., and Voia S.O., Influence of xenobiotic substances on actinomycete communities in soil, Animal Science and Biotechnologies, 45(2) (2012) 221-224.
  • [26] Lynch J.M., Microorganisms and enzymes in the soil. In: Soil Biotechnology, Microbial Factors in Crop Productivity, London: Blackwell Sci. Publ., ,1983; 185.
  • [27] Radosevich M., Traina S.J., Hao Y.I. and Touvinen O.H., Degradation and mineralization of atrazine by a soil bacterial isolate, Applied and Environmental Microbiology, 61 (1995) 297-302.
  • [28] Milosevic N.A. and Govedarica M.M., Effect of herbicides on microbiological properties of soil, Proceedings for Natural Sciences, Matica Srpska Novi Sad., 102 (2002) 5-21.
  • [29] Singh B.K., and Walker A., Microbial degradation of organophosphorus compounds, FEMS Microbiological Review, 30 (2006) 428-471.
  • [30] Lipok J., Dombrovska L., Wieczorek P. and Kafarski P., The ability of fungi isolated from stored carrot seeds to degrade organophosphonate herbicides: in pesticides in air, plant, soil and water system, Proceedings of the XII Symposium Pesticide Chemistry, (2003).
  • [31] Dibua U.M.E., Mkpuma V. O. and Enemuo S., Isolation, Characterization and Biodegradation Assay of Glyphosate Utilizing Bacteria from Exposed Rice Farm, Journal of Biology, Agriculture and Healthcare, 5(5) (2015) 96-109.
  • [32] Adelowo F.E., Olu-Arotiowa O.A. and Amuda O.S., Biodegradation of Glyphosate by Fungi Species, Advances in Bioscience and Bioengineering, 2(1) (2014) 104-118.
  • [33] Martins P.F., Martinez C.O., Giselle de Carvalho Carneiro P.I.B., Azevedo R.A., Pileggi S.A.V. and Itamar Soares de Melo, Pileggi, M., Selection of microorganisms degrading S-Metolachlor herbicide, Brazilian Archives of Biology and Technology, 50(1) (2007) 153-159.
  • [34] Ferrey M.L., Koskinen W.C., Blanchette R.A. and Burnes T.A., Mineralisation of Alachlor by lignin-degrading fungi, Canadian Journal of Microbiology, 40 (9) (1994) 795-798.

Abilities of ındigenous microorganisms to utilise herbicides for growth and as carbon source ın-vitro

Year 2020, Volume: 41 Issue: 4, 784 - 801, 29.12.2020

Adewole Sebiomo , Folake Banjo

https://doi.org/10.17776/csj.774034

Abstract

This study determined the abilities of indigenous microorganisms to utilise atrazine, xtravest, gramoxone and glyphosate as carbon source and for growth, which is a prerequisite for biodegradation and bioremediation of this herbicides in the soil. Soil treatments were carried out using the complete randomized block design for a period of 8 weeks; at company recommended rates of 4 l/h (at 350 ml in 15 l sprayer), soil treatments were carried out in triplicates. Isolation of microorganisms was done using the spread plate method on the solid mineral salts medium with each herbicide added to separate plates. The plates were incubated at 30°C for 5 days for bacteria and at 30oC for 7days for fungi. The ability of microbial isolates to utilise herbicide substrates in pure cultures were determined in minimal salt medium. B. subtilis, P. aeruginosa, P. florescences, P. putida, Aspergillus niger, A. tamarii, Fusarium oxysporum, and P. chrysogenum were isolated in all the herbicide treated soils. Bacillus subtilis recorded the highest optical density value of 1.401 on the 25th day and viable count value of 9.08 (1.21×109 cfu/ml) on the 20th day during growth on glyphosate. F. oxysporum recorded the lowest pH of 4 in gramoxone on the 25th day of incubation and the highest count of 6.10×104 cfu/g on the 20th day during atrazine utilisation. B. subtilis, A. niger and F. oxysporum showed the best abilities to utilise the herbicides for growth and as carbon source. Indigenous microorganisms used in this study successfully utilised the herbicides as carbon source and for growth. Indigenous microorganisms could be employed in the bioremediation of herbicide polluted soils. The ultimate success of bioremediation is dependent on microorganisms staying in close physical contact with substance to be degraded.

Keywords

Herbicides viable counts biodegradation bioremediation

References

  • [1] Zabaloy M.C., Garland J.L. and Gómez M.A., An integrated approach to evaluate the impacts of the herbicides glyphosate, 2,4-D and metsulfuron-methyl on soil microbial communities in the Pampas region, Argentina, Appl. Soil Ecol., 40 (2008) 1–12.
  • [2] Pampulha M.E. and Oliveira A., Impact of an herbicide combination of bromoxynil and prosulfuron on soil microorganisms. Curr. Microbiol., 53 (2006) 238–243.
  • [3] Teng Y., Luo Y.M., Sun X.H., Tu C., Xu, L., and Liu W.X. Influence of arbuscular mycorrhiza and Rhizobium on phytoremediation by alfalfa of an agricultural soil contaminated with weathered PCBs: A field study, International Journal of Phytoremediation 12 (5) (2010) 516-533.
  • [4] Zhang J. and Chiao C., Novel approaches for remediation of pesticide pollutants, International Journal Environment and Pollution, 18 (5) (2002) 423-433.
  • [5] Kearney P. and Wauchope R., Disposal options based on properties of pesticides in soil and water, in: P. Kearney, T. Roberts (Eds.), Pesticideremediation in Soils and Water, Wiley Series in Agrochemicals and Plant Protection, (1998).
  • [6] Schoefs O., Perrier M. and Samson R., Estimation of contaminant depletion in unsaturated soils using a reduced-order biodegradation model and carbon dioxide measurement. App., Microbiol. Biotechnol., 64 (2004) 256-261.
  • [7] Pointing S.B., Feasibility of bioremediation by white-rot fungi. Appl. Microbiol. Biotechnol., 57 (2001) 20-33.
  • [8] Evans C. and Hedger J., Degradation of cell wall polymers. In: Fungi in bioremediation, Cambridge;. Gadd G. Ed Cambridge University Press., U.K.,2001.
  • [9] Sasek V., Why mycoremediations have not yet come to practice. In Sasek V. et al., (Eds.) In: The utilization of bioremediation to reduce soil contamination: Problems and solutions, Kluwer Academis Publishers. 2003; 247-276.
  • [10] Johal G.S. and Huber D.M,. Glyphosate effects on diseases of plants, Eur. J. Agron., 31 (2009) 144-152.
  • [11] Mbanaso F.U., Coupe S.J., Charlesworth S.M. and Nnadi E.O., Laboratory-based experiments to investigate the impact of glyphosate-containing herbicide on pollution attenuation and biodegradation in a model pervious paving system, Chemosphere, 90 (2013) 737-746.
  • [12] Shushkova T., Ermakova I. and Leontievsky A., Glyphosate bioavailability in soil, Biodegradation, 21 (2010) 403-410.
  • [13] Solomon K.R., Anadón A., Carrasquilla G. and Cerdeira A.L., Coca and poppy eradication in Colombia: environmental and human health assessment of aerially applied glyphosate, Rev. Environ. Contam. Toxicol., 190 (2007) 43-125.
  • [14] Leckie S.E., Methods of microbial community profiling and their application to forest soils, Forest Ecol. Manag., 220 (2005) 88-106.
  • [15] Kryuchkova Y.V., Burygin G.L., Gogoleva N.E. and Gogolev Y.V., Isolation and characterization of a glyphosate-degrading rhizosphere strain, Enterobacter cloacae K7. Microbiol. Res., 169 (2014) 99-105.
  • [16] Qiao X., Ma, L., and Hummel H.E. Persistence of atrazine and occurrence of its primary metabolites in three soils, Journal of Agriculture and Food Chemistry, 14 (1996) 187-192.
  • [17] Kruger E.L, Rice P.J., Anhalt J.C., Anderson T.A. and Coats, J.R. Use ofundisturbed soil columns under controlled conditions to study the fate of [14C] diethylatrazine, Journal of Agriculture and Food Chemistry, 44 (1996) 1144-1149.
  • [18] Ayansina A.D.V. and Oso B.A., Effect of two commonly used herbicides on soil microflora at two different concentrations, African Journal of Biotechnology, 5 (2) (2006) 129-4132.
  • [19] Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T. and Williams S.T., Bergye’s Manual of Determinative Bacteriology, 9th Ed. Baltimore, Maryland: Williams and Wilkins, 1994.
  • [20] Cheesbrough M., Medical Laboratory Manual for Tropical Countries, Microbiology. Linacre House, Jordan Hill, Oxford. Vol.11., (1984).
  • [21] Moneke A.N., Okpala G.N. and Anyanwu C.U., Biodegradation of glyphosate herbicide in vitro using bacterial isolates from four rice fields, African Journal of Biotechnology, 9 (26) (2010) 4067-4074.
  • [22] Diez M.C., Biological aspects involved in the degradation of organic pollutants, J. Soil. Sci. Plant Nutrition, 10(3) (2010) 244-267.
  • [23] Chirnside A., Ritter W. and Radosevich M., Isolation of selected microbial consortium from a pesticide-contaminated mixed load site soil capable of degrading the herbicides atrazine and alachlor, Soil Biology and Biochemistry, 39 (2007) 3056-3065.
  • [24] Whitelaw-Weckert M., Hutton R., Rouse E. and Lamont R., The effect of herbicides on permanent swards on soil microbial populations, In 3rd Australian New Zealand Soils Conference, University of Sydney, Australia, (2004) 1-5.
  • [25] Filimon M.N., Popescu R., Borozan A.B., Bordean D.M., Dumitrescu G., and Voia S.O., Influence of xenobiotic substances on actinomycete communities in soil, Animal Science and Biotechnologies, 45(2) (2012) 221-224.
  • [26] Lynch J.M., Microorganisms and enzymes in the soil. In: Soil Biotechnology, Microbial Factors in Crop Productivity, London: Blackwell Sci. Publ., ,1983; 185.
  • [27] Radosevich M., Traina S.J., Hao Y.I. and Touvinen O.H., Degradation and mineralization of atrazine by a soil bacterial isolate, Applied and Environmental Microbiology, 61 (1995) 297-302.
  • [28] Milosevic N.A. and Govedarica M.M., Effect of herbicides on microbiological properties of soil, Proceedings for Natural Sciences, Matica Srpska Novi Sad., 102 (2002) 5-21.
  • [29] Singh B.K., and Walker A., Microbial degradation of organophosphorus compounds, FEMS Microbiological Review, 30 (2006) 428-471.
  • [30] Lipok J., Dombrovska L., Wieczorek P. and Kafarski P., The ability of fungi isolated from stored carrot seeds to degrade organophosphonate herbicides: in pesticides in air, plant, soil and water system, Proceedings of the XII Symposium Pesticide Chemistry, (2003).
  • [31] Dibua U.M.E., Mkpuma V. O. and Enemuo S., Isolation, Characterization and Biodegradation Assay of Glyphosate Utilizing Bacteria from Exposed Rice Farm, Journal of Biology, Agriculture and Healthcare, 5(5) (2015) 96-109.
  • [32] Adelowo F.E., Olu-Arotiowa O.A. and Amuda O.S., Biodegradation of Glyphosate by Fungi Species, Advances in Bioscience and Bioengineering, 2(1) (2014) 104-118.
  • [33] Martins P.F., Martinez C.O., Giselle de Carvalho Carneiro P.I.B., Azevedo R.A., Pileggi S.A.V. and Itamar Soares de Melo, Pileggi, M., Selection of microorganisms degrading S-Metolachlor herbicide, Brazilian Archives of Biology and Technology, 50(1) (2007) 153-159.
  • [34] Ferrey M.L., Koskinen W.C., Blanchette R.A. and Burnes T.A., Mineralisation of Alachlor by lignin-degrading fungi, Canadian Journal of Microbiology, 40 (9) (1994) 795-798.
There are 34 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Natural Sciences
Authors

Adewole Sebiomo 0000-0002-3930-0022

Folake Banjo

Publication Date December 29, 2020
Submission Date July 26, 2020
Acceptance Date November 11, 2020
Published in Issue Year 2020Volume: 41 Issue: 4

Cite

APA Sebiomo, A., & Banjo, F. (2020). Abilities of ındigenous microorganisms to utilise herbicides for growth and as carbon source ın-vitro. Cumhuriyet Science Journal, 41(4), 784-801. https://doi.org/10.17776/csj.774034