The comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats

Ahmet Şevki Taşkıran; Yaşar Taştemur

doi:10.17776/csj.735457

EN

The comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats

Abstract

Recent studies have provided important evidence that neuroinflammation has an effective role in epilepsy pathophysiology. However, it is not clear that the occurrence of neuroinflammation is related to one epileptic seizure or repeating seizures. Therefore, we aimed to investigate the comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats. In this study, 18 male Wistar albino rats were used. The animals divided into three groups as control, acute epileptic seizure model and chronic epilepsy model. Inflammatory markers (TNF-α, IL-1 β, COX-1, and COX-2) were measured by using ELISA methods in the cortical and hippocampal brain regions after completing the epileptic model procedure. Statistical evaluation of the data was performed by one-way ANOVA and multiple comparisons were determined by the Tukey test. Statistical significance was defined at p<0.05. Obtained data show that there was significant increase in inflamattory markers in chronic epilepsy model compared to the control and acute epileptic seizure model (p<0.05). In conlusion, this study may suggest that inflammatory sytem is related to epileptogenesis process rather than only one epileptic seizure.

Keywords

References

Karabulut S., Bayramov R., Korkmaz Bayramov K., Filiz A.K., Taskiran A.S. and Ozdemir E. Effect of the allopregnanolone and allotetrahydrodeoxycorticosteron on spike-wave discharges in the EEG of absence epilepsy rat models. Gen Physiol Biophys., 37 (2) (2018) 205-211.
Jackson C.F., Makin S.M., Marson A.G. and Kerr M. Pharmacological interventions for epilepsy in people with intellectual disabilities. Cochrane Database Syst Rev., 3(9) (2015) CD005399
Akkaya R., Gümüş E., Akkaya B., Karabulut S., Gülmez K., Karademir M., Taştemur Y. and Taşkıran A.Ş. Wi-Fi decreases melatonin protective effect and increases hippocampal neuronal damage in pentylenetetrazole induced model seizures in rats. Pathophysiology., 26 (3-4) (2019) 375-379.
Wiebe S., Blume W.T., Girvin J.P. and Eliasziw M. Effectiveness and Efficiency of Surgery for Temporal Lobe Epilepsy Study Group. A randomized, controlled trial of surgery for temporal-lobe epilepsy. N Engl J Med., 345 (5) (20013) 11-8.
Dey A., Kang X., Qiu J., Du Y. and Jiang J. Anti-Inflammatory Small Molecules To Treat Seizures and Epilepsy: From Bench to Bedside. Trends Pharmacol Sci., 37 (6) (2016) 463-484
Vezzani A., Aronica E., Mazarati A. and Pittman Q.J. Epilepsy and brain inflammation. Exp Neurol., 244 (2013) 11-21.
Rana A. and Musto A.E. The role of inflammation in the development of epilepsy. J Neuroinflammation., 15 (1) (2018) 144.
Shimada T. and Yamagata K. Pentylenetetrazole-Induced Kindling Mouse Model. J Vis Exp., 12 (136) (2018) 56573.

Romualdi P., Lesa G., Donatini A., Balboni G., Tomatis R. and Ferri S. Alterations in vasoactive intestinal polypeptide-related peptides after pentylenetetrazole-induced seizures in rat brain. Eur J Pharmacol., 229 (2-3) (1992) 149-153.
Kruger N.J. The Bradford method for protein quantitation. Methods Mol Biol., 32 (1994) 9-15.
Alyu F. and Dikmen M. Inflammatory aspects of epileptogenesis: Contribution of molecular inflammatory mechanisms. Acta Neuropsychiatr., 29 (1) (2017)1-16.
Scorza C.A., Marques M.J.G., Gomes da Silva S., Naffah-Mazzacoratti M.D.G., Scorza F.A. and Cavalheiro E.A. Status epilepticus does not induce acute brain inflammatory response in the Amazon rodent Proechimys, an animal model resistant to epileptogenesis. Neurosci. Lett., 668 (2018) 169-173.
Stellwagen D. and Malenka R.C. Synaptic scaling mediated by glial TNF-αlpha. Nature., 440 (7087) (2006) 1054-1059.
Galic M.A., Riazi K. and Pittman Q.J. Cytokines and brain excitability. Front. Neuroendocrino., 33 (1) (2012) 116-125.
Dede F., Karadenizli S., Özsoy Ö.D., Eraldemir F.C., Şahin D. and Ateş N. The Effects of Adenosinergic Modulation on Cytokine Levels in a Pentylenetetrazole-Induced Generalized Tonic-Clonic Seizure Model. Neuroimmunomodulation., 24 (1) (2017) 54–59.
Golechha M., Sarangal V., Bhatia J., Chaudhry U., Saluja D. and Arya D.S. Naringin ameliorates pentylenetetrazol-induced seizures and associated oxidative stress, inflammation, and cognitive impairment in rats: possible mechanisms of neuroprotection. Epilepsy Behav., 41(2014) 98–102.
Chen L., Liu X., Wang H. and Qu M. Gastrodin Attenuates Pentylenetetrazole-Induced Seizures by Modulating the Mitogen-Activated Protein Kinase-Associated Inflammatory Responses in Mice. Neurosci Bull., 33 (3) (2017) 264–272.
Mansoor S.R., Hashemian M., Khalili-Fomeshi M., Ashrafpour M., Moghadamnia A.A. and Ghasemi-Kasman M. Upregulation of klotho and erythropoietin contributes to the neuroprotection induced by curcumin-loaded nanoparticles in experimental model of chronic epilepsy. Brain Res Bull., 142 (2018) 281–288.
Ahmadian S.R., Ghasemi-Kasman M., Pouramir M. and Sadeghi F. Arbutin attenuates cognitive impairment and inflammatory response in pentylenetetrazol-induced kindling model of epilepsy. Neuropharmacology., 146 (2019) 117–127.
Muke S., Kaikini A., Peshattiwar V., Bagle S., Dighe V. and Sathaye S. Neuroprotective Effect of Coumarin Nasal Formulation: Kindling Model Assessment of Epilepsy. Front Pharmacol., 9 (2018) 992.
Hoda U., Agarwal N.B., Vohora D., Parvez S. and Raisuddin S. Resveratrol suppressed seizures by attenuating IL-1β, IL1-Ra, IL-6, and TNF-α in the hippocampus and cortex of kindled mice. Nutr Neurosci., 20 (9) (2017) 497–504.
Postnikova T., Zubareva O., Kovalenko A., Kim K., Magazanik L. and Zaitsev A. Status epilepticus impairs synaptic plasticity in rat hippocampus and is followed by changes in expression of NMDA receptors. Biochem Mosc., 82 (3) (2017) 282–290. Singh N., Saha L., Kumari P., Singh J., Bhatia A., Banerjee D. and Chakrabarti A. Effect of dimethyl fumarate on neuroinflammation and apoptosis in pentylenetetrazol kindling model in rats. Brain Res Bull., 144 (2019) 233–245.
Sutula T., Koch J., Golarai G., Watanabe Y. and McNamara J.O. NMDA receptor dependence of kindling and mossy fibersprouting: evidence that the NMDA receptor regulates pattern-ing of hippocampal circuits in the adult brain. J. Neurosci., 16 (1996) 7398-7406.
Davoudi M., Shojaei A., Palizvan M.R., Javan M. and Mirnajafi-Zadeh J. Comparison between standard protocol and a novel window protocol for induction of pentylenetetrazol kindled seizures in the rat. Epilepsy Res., 106 (1-2) (2013) 54–63.
Tanaka S., Nakamura T., Sumitani K., Takahashi F., Konishi R., Itano T. and Miyamoto O. Stage- and region-specific cyclooxygenase expression and effects of a selective COX-1 inhibitor in the mouse amygdala kindling model. Neurosci Res., 65 (1) (2009) 79-87.
Ma L., Cui X.L., Wang Y., Li X.W., Yang F., Wei D. and Jiang W. Aspirin attenuates spontaneous recurrent seizures and inhibits hippocampal neuronal loss, mossy fiber sprouting and aberrant neurogenesis following pilocarpine-induced status epilepticus in rats. Brain Res., 1469 (2012) 103-113.
Barbalho P.G., Lopes-Cendes I. and Maurer-Morelli C.V. Indomethacin treatment prior to pentylenetetrazole-induced seizures downregulates the expression of il1b and cox2 and decreases seizure-like behavior in zebrafish larvae. BMC Neurosci., 17 (2016) 12.
Kulkarni S.K. and Dhir A. Cyclooxygenase in epilepsy: from perception to application. Drugs Today (Barc)., 45(2) (2009) 135-154.
Citraro R., Leo A., Marra R., De Sarro G. and Russo E. Antiepileptogenic effects of the selective COX-2 inhibitor etoricoxib, on the development of spontaneous absence seizures in WAG/Rij rats. Brain Res Bull., 113 (2015) 1-7.
Tu B. and Bazan N.G. Hippocampal kindling epileptogenesis upregulates neuronal cyclooxygenase-2 expression in neocortex. Exp Neurol., 179 (2) (2003) 167-175.
Takemiya T., Suzuki K., Sugiura H., Yasuda S., Yamagata K., Kawakami Y. and Maru E. Inducible brain COX-2 facilitates the recurrence of hippocampal seizures in mouse rapid kindling. Prostaglandins Other Lipid Mediat., 71(3-4) (2003) 205-216.
Baik E.J., Kim E.J., Lee S.H. and Moon C. Cyclooxygenase-2 selective inhibitors aggravate kainic acid induced seizure and neuronal cell death in the hippocampus. Brain Res., 843 (1-2) (1999) 118-129.
Kim H.J., Chung J.I., Lee S.H., Jung Y.S., Moon C.H. and Baik E.J. Involvement of endogenous prostaglandin F2alpha on kainic acid-induced seizure activity through FP receptor: the mechanism of proconvulsant effects of COX-2 inhibitors. Brain Res., 1193 (2008) 153-161.

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ahmet Şevki Taşkıran ^*
0000-0002-5810-8415
Türkiye

Yaşar Taştemur
0000-0002-2016-0592
Türkiye

Publication Date

September 30, 2020

Submission Date

May 11, 2020

Acceptance Date

August 25, 2020

Published in Issue

Year 1970 Volume: 41 Number: 3

DOI

https://doi.org/10.17776/csj.735457

IZ

https://izlik.org/JA82SG35BG

Cite

RIS / Bibtex

APA

Taşkıran, A. Ş., & Taştemur, Y. (2020). The comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats. Cumhuriyet Science Journal, 41(3), 635-641. https://doi.org/10.17776/csj.735457