Neurodegenerative diseases (NDs) are characterized by loss of neurons. NDs are associated with development of inflammation. Existing drugs against NDs only delay the progression; however, they do not provide a cure. The studies for the treatment of NDs focused on to discover natural products that have the potential of anti-inhibition and anti-radical properties. The Papaveraceae family members are important for the synthesis of pharmaceutically compounds such as flavonoids which act like anti-inflammatory drugs. In this study, methanol and water extracts of Glaucium corniculatum, a member of the Papaveraceae family, were analysed for flavonoid compounds. The effects of extracts on neuronal PC12 cells viability was determined. The anti-inflammatory effects of extracts were assessed by measuring the levels of IL-6 and IL-10 cytokines on hydrogen peroxide (H2O2)-stimulated PC12 cells. As a result of our studies, Rutin and Quercetin flavonoids have been found to be as major. The amount of Rutin was higher in methanol (45 μg/ml) than water (41 μg/ml). Quercetin was better extracted with methanol (12 μg/ml) than water (10 μg/ml). None of the tested extracts were cytotoxic even to PC12 cells. Both extracts showed an anti-inflammatory effect in a dose dependent manner. The water extract showed the maximum anti-inflammatory effect, with IL-6 secretion decreased 79 fold according to the H2O2 treated group and IL-10 secretion increased to 87 fold according to the control group. This study is an evidence that the Rutin and Quercetin flavonoids detected in G. corniculatum methanol and water extracts have a neuroprotective effect through anti-inflammation.
Glaucium corniculatım flavonoid neurodegenerative diseases anti-inflammation
Neurodegenerative
diseases (NDs) are characterized by loss of neurons. NDs are associated with development
of inflammation. Existing drugs against NDs only delay the progression; however,
they do not provide a cure. The studies for the treatment of NDs focused on to discover
natural products that have the potential of anti-inhibition and anti-radical properties.
The Papaveraceae family members are important
for the synthesis of pharmaceutically compounds such as flavonoids which act like
anti-inflammatory drugs. In this study, methanol and water extracts of Glaucium corniculatum, a member of the Papaveraceae family, were analysed for flavonoid
compounds. The effects of extracts on neuronal PC12 cells viability was determined.
The anti-inflammatory effects of extracts were assessed by measuring the levels
of IL-6 and IL-10 cytokines on hydrogen peroxide (H2O2)-stimulated
PC12 cells. As a result of our studies, Rutin and Quercetin flavonoids have been
found to be as major. The amount of Rutin was higher in methanol (45 μg/ml) than
water (41 μg/ml). Quercetin was better extracted with methanol (12 μg/ml) than water
(10 μg/ml). None of the tested extracts were cytotoxic even to PC12 cells. Both
extracts showed an anti-inflammatory effect in a dose dependent manner. The water
extract showed the maximum anti-inflammatory effect, with IL-6 secretion decreased
79 fold according to the H2O2 treated group and IL-10 secretion
increased to 87 fold according to the control group. This study is an evidence that
the Rutin and Quercetin flavonoids detected in G. corniculatum methanol and water extracts have a neuroprotective effect
through anti-inflammation.
Glaucium corniculatım flavonoid neurodegenerative diseases anti-inflammation
Birincil Dil | İngilizce |
---|---|
Konular | Yapısal Biyoloji |
Bölüm | Makaleler |
Yazarlar | |
Yayımlanma Tarihi | 25 Kasım 2017 |
Gönderilme Tarihi | 4 Mayıs 2017 |
Yayımlandığı Sayı | Yıl 2017 Cilt: 4 Sayı: 3, Special Issue 1 |