Abstract
Bu çalışmada, Smilax excelsa L. meyve ektraktının antimikrobiyal, antioksidan ve
antimutajenik aktiviteleri araştırılmıştır. Antimikrobiyal aktivite disk
difüzyon yöntemi ile antimutajenik aktivite ise Ames/Salmonella/mikrozom testi
kullanılarak araştırılmıştır. S. excelsa
meyve örneklerinin antioksidan özelliği ise toplam fenolik ve flavonoid
içeriğinin tespiti ve 1,1-difenil-2-pikrilhidrazil (DPPH) giderme etkisi
araştırılarak belirlenmiştir. Meyve ekstraktının 200 mg plak-1
dozunda Salmonella typhimirium TA1535
suşu ile S9 karışımı yokluğunda %81, S9 karışımı varlığında ise %67 oranında
mutajenite inhibisyonu oluşturduğu belirlenmiştir. S. excelsa meyve eksraktının test edilen mikroorganizmalara karşı
11-16 cm aralığında inhibisyon zonu oluşturduğu gözlenmiştir. 200 mg mL-1
konsantrasyonunda ekstraktta flavonoid içeriği 0.7985±0.0124 mgQE 100 mL-1
olarak, fenolik içerik ise 11.9847±0.0041 mgGAE 100 mL-1 olarak
tespit edilmiştir. 200 mg L-1 dozunda DPPH giderme oranı %55 olarak
belirlenmiştir. Sonuç olarak, S. excelsa meyve
dokularının yüksek antimutajenik aktivite sergilediği, potansiyel doğal bir
antimutajenik ve antioksidan kaynak olduğu olduğu belirlenmiştir.
References
- Stein C.M., Are herbal products dietary supplements or drugs? An important question for public safety, Clin. Pharmacol. Ther., 71-6 (2002) 411-413.
- Süzer Ö., Serotonin, Serotonin Reseptörlerinin Baslica Etki Yerleri Ve Fonksiyonlari. Süzer Farmakoloji. 3. baskı. Istanbul: Klinisyen Tip Kitabevleri, (2005); pp150-154.
- IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Address: https://monographs.iarc.fr/wp-content/uploads/2018/06/mono94-4.pdf. Retrieved January 9, 2019.
- Navarro M., Montilla M.P., Cabo M.M., Galisteo M., Cáceres A., Morales C. and Berger I., Antibacterial, antiprotozoal and antioxidant activity of five plants used in Izabal for infectious diseases, Phytother. Res., 1 (2003) 325–329.
- Lee S.E., Ju E.M. and Kim J.H., Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root, Exp. Mol. Med., 33 (2001) 263-268.
- Jiang J. and Xu Q., Immunomodulatory activity of the aqueous extract from rhizome of Smilax glabra in the later phase of adjuvant-induced arthritis in rats, J. Ethnopharmacol., 85 (2003) 53-59.
- Ozbucak T.B., Ergen A.O. and Yalcin S., Nutrition contents of the some wild edible plants in Central Black Sea region of Turkey, IJNES, 1 (2007) 11-13.
- Ozsoy N., Can A., Yanardag R. and Akev N., Antioxidant activity of Smilax excelsa L. leaf extracts, Food Chem., 110 (2008) 571-583.
- Ozsoy N., Okyar A., Arda-Pirincci P., Can A., Bolkent S. and Akev N., Evaluation of Smilax excelsa L. use in experimentally induced nephrotoxicity, J. Fac. Vet. Med. Kafkas University, 19-5 (2013) 807-814.
- Efe E. Smilax excelsa L. meyve ekstrelerinin antimutajenik, antimikrobiyal ve antioksidan aktivitelerinin araştırılması. Giresun University Graduate School of Natural and Applied Sciences, Deparment of Biology, Master Thesiss. 16 (2017).
- Cooper D.G. and Paddock P.A., Production of a biosurfactant from Torulopsis bombicola, Appl. Environ. Microbiol., 47-1 (1984) 173-176.
- Singleton V.L. and Rossi J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Viticult., 16 (1965) 144-158.
- Zhishen J., Mengcheng T. and Jianming W., The determination of flavonoid contents on mulberry and their scavenging effects on superoxide radical, Food Chem., 64 (1999) 555-559.
- Yen G.C. and Duh P.D., Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species, J. Agric. Food Chem., 42 (1994) 629-632.
- Maron D.M. and Ames B.N., Revised methods for the mutagenicity test, Mutat. Res., 113(1983) 173-215.
- Uysal A., Zengin G., Durak Y. and Aktumsek A., Screening for antioxidant and antimutagenic properties of extracts from Centaurea pterocaula as well as their enzyme inhibitory potentials, Marmara Pharm. J., 20-3 (2016) 232-242.
- Khaligh P., Salehi P., Farimani M.M., Ali-Asgari S., Esmaeili M.A. and Ebrahimi S.M., Bioactive compounds from Smilax excelsa L, J. Iran Chem. Soc., 13 (2016) 1055-1059.
- Ivanova A., Mikhova B., Kostova I. and Evstatieva L., Bioactive chemical constituents from Smilax excels, Chem. Nat. Compd., 46 (2010) 295-297.
- Schultes R.E., The Kingdom of Plants. In: Thomson WAR., editor. Medicines from the earth. New York: McGraw-Hill Book Co, 1978; pp 208.
- Feng Q.L., Wu J., Chen G.Q., Cui F., Kim T.N., and Kim J.O., A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus, J. Biomed. Mate. Res., 52-4 (2000) 662-668.
- Lu C.L., Zhu W., Wang M., Xu X.J. and Lu C.J., Antioxidant and anti-inflammatory activities of phenolic-enriched extracts of Smilax glabra, J. Evid. Based Complement Altern. Med., 2014 (2014) 1-8.
- Shuo X., Ming-Ying S., Guang-Xue L., Feng X., Xuan W., Cheng-Chao S. and Shao-Qing C., Chemical Constituents from the Rhizomes of Smilax glabra and their antimicrobial activity, Molecules, 18 (2013) 5265-5287.
Abstract
In this study, antimutagenic, antimicrobial and antioxidant activities
of Smilax excelsa L. fruit extract
were evaluated. The antimicrobial effect
was investigated by disk diffusion method and the antimutagenic effect was
investigated by Ames/Salmonella/microsom test. The antioxidant properties of S.
excelsa fruit samples were determined by investigating the total phenolic,
flavonoid contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical
scavenging activity. It was observed
that 200 mg plate-1 fruit extract was found to have a mutagenicity
inhibition as 81% in the absence of S9 mixture and 67% in the presence of S9
mixture against Salmonella typhimurium
TA1535. S. excelsa fruit extract
produced an inhibition zone in the range of 11-16 cm against the tested
microorganisms. Flavonoid and phenolic contents were found to be 0.7985±0.0124
mgQE 100 mL-1 and 11.9847±0.0041 mgGAE 100 mL-1 at the
200 mg mL-1 extract concentration, respectively. The DPPH removal
rate was determined to be 55% at the 200 mg L-1 dose. As a result,
it was observed that the S. excelsa fruit
tissues exhibited a highly antimutagenic activity and has been determined as a
potential natural antimicrobial and antioxidant source.
References
- Stein C.M., Are herbal products dietary supplements or drugs? An important question for public safety, Clin. Pharmacol. Ther., 71-6 (2002) 411-413.
- Süzer Ö., Serotonin, Serotonin Reseptörlerinin Baslica Etki Yerleri Ve Fonksiyonlari. Süzer Farmakoloji. 3. baskı. Istanbul: Klinisyen Tip Kitabevleri, (2005); pp150-154.
- IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Address: https://monographs.iarc.fr/wp-content/uploads/2018/06/mono94-4.pdf. Retrieved January 9, 2019.
- Navarro M., Montilla M.P., Cabo M.M., Galisteo M., Cáceres A., Morales C. and Berger I., Antibacterial, antiprotozoal and antioxidant activity of five plants used in Izabal for infectious diseases, Phytother. Res., 1 (2003) 325–329.
- Lee S.E., Ju E.M. and Kim J.H., Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root, Exp. Mol. Med., 33 (2001) 263-268.
- Jiang J. and Xu Q., Immunomodulatory activity of the aqueous extract from rhizome of Smilax glabra in the later phase of adjuvant-induced arthritis in rats, J. Ethnopharmacol., 85 (2003) 53-59.
- Ozbucak T.B., Ergen A.O. and Yalcin S., Nutrition contents of the some wild edible plants in Central Black Sea region of Turkey, IJNES, 1 (2007) 11-13.
- Ozsoy N., Can A., Yanardag R. and Akev N., Antioxidant activity of Smilax excelsa L. leaf extracts, Food Chem., 110 (2008) 571-583.
- Ozsoy N., Okyar A., Arda-Pirincci P., Can A., Bolkent S. and Akev N., Evaluation of Smilax excelsa L. use in experimentally induced nephrotoxicity, J. Fac. Vet. Med. Kafkas University, 19-5 (2013) 807-814.
- Efe E. Smilax excelsa L. meyve ekstrelerinin antimutajenik, antimikrobiyal ve antioksidan aktivitelerinin araştırılması. Giresun University Graduate School of Natural and Applied Sciences, Deparment of Biology, Master Thesiss. 16 (2017).
- Cooper D.G. and Paddock P.A., Production of a biosurfactant from Torulopsis bombicola, Appl. Environ. Microbiol., 47-1 (1984) 173-176.
- Singleton V.L. and Rossi J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Viticult., 16 (1965) 144-158.
- Zhishen J., Mengcheng T. and Jianming W., The determination of flavonoid contents on mulberry and their scavenging effects on superoxide radical, Food Chem., 64 (1999) 555-559.
- Yen G.C. and Duh P.D., Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species, J. Agric. Food Chem., 42 (1994) 629-632.
- Maron D.M. and Ames B.N., Revised methods for the mutagenicity test, Mutat. Res., 113(1983) 173-215.
- Uysal A., Zengin G., Durak Y. and Aktumsek A., Screening for antioxidant and antimutagenic properties of extracts from Centaurea pterocaula as well as their enzyme inhibitory potentials, Marmara Pharm. J., 20-3 (2016) 232-242.
- Khaligh P., Salehi P., Farimani M.M., Ali-Asgari S., Esmaeili M.A. and Ebrahimi S.M., Bioactive compounds from Smilax excelsa L, J. Iran Chem. Soc., 13 (2016) 1055-1059.
- Ivanova A., Mikhova B., Kostova I. and Evstatieva L., Bioactive chemical constituents from Smilax excels, Chem. Nat. Compd., 46 (2010) 295-297.
- Schultes R.E., The Kingdom of Plants. In: Thomson WAR., editor. Medicines from the earth. New York: McGraw-Hill Book Co, 1978; pp 208.
- Feng Q.L., Wu J., Chen G.Q., Cui F., Kim T.N., and Kim J.O., A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus, J. Biomed. Mate. Res., 52-4 (2000) 662-668.
- Lu C.L., Zhu W., Wang M., Xu X.J. and Lu C.J., Antioxidant and anti-inflammatory activities of phenolic-enriched extracts of Smilax glabra, J. Evid. Based Complement Altern. Med., 2014 (2014) 1-8.
- Shuo X., Ming-Ying S., Guang-Xue L., Feng X., Xuan W., Cheng-Chao S. and Shao-Qing C., Chemical Constituents from the Rhizomes of Smilax glabra and their antimicrobial activity, Molecules, 18 (2013) 5265-5287.
Details
| Primary Language | English |
|---|---|
| Journal Section | Natural Sciences |
| Authors | |
| Publication Date | June 30, 2019 |
| Submission Date | January 16, 2019 |
| Acceptance Date | April 25, 2019 |
| Published in Issue | Year 2019 |