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Biological Activity Evaluation of Scorzonera tomentosa L.

Year 2023, Volume: 10 Issue: 2, 162 - 167, 31.07.2023

Gülşen Güçlü Nuraniye Eruygur Esra Uçar Dilara Ülger Özbek Halil Bal Aşkın Akpolat Danial Kahrizi

https://doi.org/10.19159/tutad.1278795

Abstract

This study aimed to evaluate the phytochemical components, antimicrobial activity, and antioxidant activity of 80% ethanol extract of Scorzonera tomentosa, an endemic species. The chemical constituents of the ethanolic extract of S. tomentosa was primarily characterized by gas-chromatography-mass spectrometry analysis (GC-MS), ten components were identified. The major component was found as 2-pentanamine (35.68%). When the antioxidant capacity of S. tomentosa was examined, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities were determined to be quite high compared to the reference drug (IC50 values; DPPH: 517.0 ± 1.86 µg mL-1; ABTS: 244.8 ± 0.94 µg mL-1; reference drug: 1.313 ± 0.24 µg mL-1). In addition, according to total phenol content and total flavonoid content analyses, it was determined that the plant is richer in flavonoids. The antimicrobial activity of this species is not at an effective level. More extensive studies with S. tomentosa may allow the plant to be used as a natural antioxidant.

Keywords

Scorzonera tomentosa Antioksidan kapasite chemical content antimicrobial activity

References

  • Abdelkader, H.B., Salah, K.B.H., Liouane, K., Boussaada, O., Gafsi, K., Mahjoub, M.A., Aouni, M., Hellal, A.N., Mighri, Z., 2010. Antimicrobial activity of Rhaponticum acaule and Scorzonera undulata growing wild in Tunisia. African Journal of Microbiology Research, 4(19): 1954-1958.
  • Ak, G., Zengin, G., 2021. Evaluation of the fatty acid profiles of the aerial and root parts of three Scorzonera L. taxa. Turkish Journal of Nature and Science, 10(1): 166-170. (In Turkish).
  • Ak, G., Zengin, G., Dall’Acqua, S., Ferrarese, I., Sut, S., Glamočlija, J., Soković, M., Nenadić, M., Chiavaroli, A., Recinella, L., Leone, S., Orlando, G., Menghini, L., Ferrante, C., 2022. A new step on the chemical profiles and pharmacological effects of three scorzonera species (S. hieraciifolia, S. hispanica and S. tomentosa). Plant Biosystems-An International Journal Dealing with All Aspects of Plant Biology, 157(1): 119-128.
  • Akkol, E.K., Šmejkal, K., Kurtul, E., Ilhan, M., Güragac, F.T., İşcan, G.S., Bahadır-Acıkara, Ö., Cvačka, J., Buděšínský, M., 2019. Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process. Journal of Ethnopharmacology, 245: 112168.
  • Bahadır Acıkara, Ö., Saltan Çitoğlu, G., Çoban, T., 2013a. Phytochemical screening and sntioxidant activities of selected Scorzonera species. Turkish Journal of Pharmaceutical Sciences, 10(3): 453-462.
  • Bahadır Acıkara, Ö., Saltan Çitoğlu, G., Gençler Özkan, A.M., 2013b. Qualitative and quantitative analysis of phenolic acids in Scorzonera tomentosa L. Turkish Journal of Pharmaceutical Sciences, 10(1): 1-8.
  • Bahadır-Acıkara, Ö., Özbilgin, S., Saltan-İşcan, G., Dall’Acqua, S., Rjašková, V., Özgökçe, F., Suchý, V., Šmejkal, K., 2018. Phytochemical analysis of podospermum and Scorzonera n-hexane extracts and the HPLC quantitation of triterpenes. Molecules, 23(7): 1813.
  • Boussaada, O., Saidana, D., Chriaa, J., Chraif, I., Ammar, Mahjoub, M.A., Mighri, Z., Daami, M., Helal, A.N., 2008. Chemical composition and antimicrobial activity of volatile components of Scorzonera undulata. Journal of Essential Oil Research, 20(4): 358-362.
  • Clarke, G., Ting, K.N., Wiart, C., Fry, J., 2013. High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants, 2(1): 1-10.
  • Csupor-Löffler, B., Hajdú, Z., Réthy, B., Zupkó, I., Máthé, I., Rédei, T., Falkay, G., Hohmann, J., 2009. Antiproliferative activity of Hungarian asteraceae species against human cancer cell lines: part II. Phytotherapy Research, 23(8): 1109-1115.
  • Duran, A., Hamzaoğlu, E., 2004. A new species of Scorzonera L. (Asteraceae) from South Anatolia, Turkey. Biologia Bratislava, 59(1): 47-50.
  • Eloff, J., 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica, 64(08): 711-713.
  • Erden, Y., Kırbağ, S., Yılmaz, Ö., 2012. Phytochemical composition and antioxidant activity of some Scorzonera species. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 83(2): 271-276.
  • Eruygur, N., Ucar, E., Akpulat, H.A., Shahsavari, K., Safavi, S.M., Kahrizi, D., 2019. In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Molecular Biology Reports, 46(2): 2121-2129.
  • Harkati, B., Akkal, S., Bayat, C., Laouer, H., Franca, M.D., 2010. Secondary metabolites from Scorzonera undulata ssp. deliciosa (Guss.) Maire (Asteracae) and their antioxidant activities. Records of Natural Products, 4(3): 171-175.
  • Küpeli Akkol, E., Bahadır-Acıkara, Ö., Süntar, I., Citoğlu-Saltan, G., Keleş, H., Ergene, B., 2011. Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method. Journal of Ethnopharmacology, 137(2): 1018-1027.
  • Lendzion, K., Gornowicz, A., Bielawski, K., Bielawska, A., 2021. Phytochemical composition and biological activities of Scorzonera species. International Journal of Molecular Sciences, 22(10): 1-42.
  • Milella, L., Bader, A., De Tommasi, N., Russo, D., Braca, A., 2014. Antioxidant and free radical-scavenging activity of constituents from two Scorzonera species. Food Chemistry, 160: 298-304.
  • Molan, A.L., Mahdy, A.S., 2014. Iraqi medicinal plants: Total flavonoid contents, free-radical scavenging and bacterial beta-glucuronidase inhibition activities. IOSR Journal of Dental and Medical Sciences, 13(5): 72-77.
  • Nasseri, M.A., Bigy, S.S., Allahresani, A., Malekaneh, M., 2015. Assessment of antioxidant activity, chemical characterization and evaluation of fatty acid compositions of Scorzonera paradoxa Fisch and CA Mey. Jundishapur Journal of Natural Pharmaceutical Products, 10(4): 1-5.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(10): 1231-1237.
  • Olszowy, M., 2019. What is responsible for antioxidant properties of polyphenolic compounds from plants? Plant Physiology and Biochemistry, 144: 135-143.
  • Petkova, N., 2018. Characterization of inulin from black salsify (Scorzonera hispanica L.) for food and pharmaceutical purposes. Asian Journal of Pharmaceutical and Clinical Research, 11(12): 221-225.
  • Sarı, A., Şahin, H., Özsoy, N., Özbek Çelik, B., 2019. Phenolic compounds and in vitro antioxidant, anti-inflammatory, antimicrobial activities of Scorzonera hieraciifolia Hayek roots. South African Journal of Botany, 125: 116-119.
  • Sarı, A., Zidorn, C., Ellmerer, E.P., Özgökçe, F., Ongania, K.H., Stuppner, H., 2007. Phenolic compounds from Scorzonera tomentosa L. Helvetica Chimica Acta, 90(2): 311-317.
  • Sweidan, A., El-Mestrah, M., Kanaan, H., Dandache, I., Merhi, F., Chokr, A., 2020. Antibacterial and antibiofilm activities of Scorzonera mackmeliana. Pakistan Journal of Pharmaceutical Sciences, 33(1): 199-206.
  • Taşkın, D., Gecim, M., Doğan, A., Beceren, A., 2021. Polyphenolic composition and antioxidant effect of aerial parts and roots extracts from Scorzonera veratrifolia. International Journal of Secondary Metabolite, 8(3): 284-299.
  • Tsevegsuren, N., Edrada, R.., Lin, W., Ebel, R., Torre, C., Ortlepp, S., Wray, V., Proksch, P., 2007. Biologically active natural products from Mongolian medicinal plants scorzonera divaricata and scorzonera pseudodivaricata. Journal of Natural Products, 70(6): 962967.
  • Wang, Y., Edrada-Ebel, R.A., Tsevegsuren, N., Sendker, J., Braun, M., Wray, V., Lin, W., Proksch, P., 2009. Dihydrostilbene derivatives from the Mongolian medicinal plant Scorzonera radiata. Journal of Natural Products, 72(4): 671-675.
  • Wu, Q.-X., He, X.-F., Jiang, C.-X., Zhang, W., Shi, Z.-N., Li, H.-F., Zhu, Y., 2018. Two novel bioactive sulfated guaiane sesquiterpenoid salt alkaloids from the aerial parts of Scorzonera divaricata. Fitoterapia, 124: 113-119.
  • Yang, Y.-J., Yao, J., Jin, X.-J., Shi, Z.-N., Shen, T.-F., Fang, J.-G., Yao, X.-J., Zhu, Y., 2016. Sesquiterpenoids and tirucallane triterpenoids from the roots of Scorzonera divaricata. Phytochemistry, 124: 86-98.

Biological Activity Evaluation of Scorzonera tomentosa L.

Year 2023, Volume: 10 Issue: 2, 162 - 167, 31.07.2023

Gülşen Güçlü Nuraniye Eruygur Esra Uçar Dilara Ülger Özbek Halil Bal Aşkın Akpolat Danial Kahrizi

https://doi.org/10.19159/tutad.1278795

Abstract

This study aimed to evaluate the phytochemical components, antimicrobial activity, and antioxidant activity of 80% ethanol extract of Scorzonera tomentosa, an endemic species. The chemical constituents of the ethanolic extract of S. tomentosa was primarily characterized by gas-chromatography-mass spectrometry analysis (GC-MS), ten components were identified. The major component was found as 2-pentanamine (35.68%). When the antioxidant capacity of S. tomentosa was examined, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities were determined to be quite high compared to the reference drug (IC50 values; DPPH: 517.0 ± 1.86 µg mL-1; ABTS: 244.8 ± 0.94 µg mL-1; reference drug: 1.313 ± 0.24 µg mL-1). In addition, according to total phenol content and total flavonoid content analyses, it was determined that the plant is richer in flavonoids. The antimicrobial activity of this species is not at an effective level. More extensive studies with S. tomentosa may allow the plant to be used as a natural antioxidant.

Keywords

Scorzonera tomentosa Antioxidant capacity chemical content antimicrobial activity

References

  • Abdelkader, H.B., Salah, K.B.H., Liouane, K., Boussaada, O., Gafsi, K., Mahjoub, M.A., Aouni, M., Hellal, A.N., Mighri, Z., 2010. Antimicrobial activity of Rhaponticum acaule and Scorzonera undulata growing wild in Tunisia. African Journal of Microbiology Research, 4(19): 1954-1958.
  • Ak, G., Zengin, G., 2021. Evaluation of the fatty acid profiles of the aerial and root parts of three Scorzonera L. taxa. Turkish Journal of Nature and Science, 10(1): 166-170. (In Turkish).
  • Ak, G., Zengin, G., Dall’Acqua, S., Ferrarese, I., Sut, S., Glamočlija, J., Soković, M., Nenadić, M., Chiavaroli, A., Recinella, L., Leone, S., Orlando, G., Menghini, L., Ferrante, C., 2022. A new step on the chemical profiles and pharmacological effects of three scorzonera species (S. hieraciifolia, S. hispanica and S. tomentosa). Plant Biosystems-An International Journal Dealing with All Aspects of Plant Biology, 157(1): 119-128.
  • Akkol, E.K., Šmejkal, K., Kurtul, E., Ilhan, M., Güragac, F.T., İşcan, G.S., Bahadır-Acıkara, Ö., Cvačka, J., Buděšínský, M., 2019. Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process. Journal of Ethnopharmacology, 245: 112168.
  • Bahadır Acıkara, Ö., Saltan Çitoğlu, G., Çoban, T., 2013a. Phytochemical screening and sntioxidant activities of selected Scorzonera species. Turkish Journal of Pharmaceutical Sciences, 10(3): 453-462.
  • Bahadır Acıkara, Ö., Saltan Çitoğlu, G., Gençler Özkan, A.M., 2013b. Qualitative and quantitative analysis of phenolic acids in Scorzonera tomentosa L. Turkish Journal of Pharmaceutical Sciences, 10(1): 1-8.
  • Bahadır-Acıkara, Ö., Özbilgin, S., Saltan-İşcan, G., Dall’Acqua, S., Rjašková, V., Özgökçe, F., Suchý, V., Šmejkal, K., 2018. Phytochemical analysis of podospermum and Scorzonera n-hexane extracts and the HPLC quantitation of triterpenes. Molecules, 23(7): 1813.
  • Boussaada, O., Saidana, D., Chriaa, J., Chraif, I., Ammar, Mahjoub, M.A., Mighri, Z., Daami, M., Helal, A.N., 2008. Chemical composition and antimicrobial activity of volatile components of Scorzonera undulata. Journal of Essential Oil Research, 20(4): 358-362.
  • Clarke, G., Ting, K.N., Wiart, C., Fry, J., 2013. High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants, 2(1): 1-10.
  • Csupor-Löffler, B., Hajdú, Z., Réthy, B., Zupkó, I., Máthé, I., Rédei, T., Falkay, G., Hohmann, J., 2009. Antiproliferative activity of Hungarian asteraceae species against human cancer cell lines: part II. Phytotherapy Research, 23(8): 1109-1115.
  • Duran, A., Hamzaoğlu, E., 2004. A new species of Scorzonera L. (Asteraceae) from South Anatolia, Turkey. Biologia Bratislava, 59(1): 47-50.
  • Eloff, J., 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica, 64(08): 711-713.
  • Erden, Y., Kırbağ, S., Yılmaz, Ö., 2012. Phytochemical composition and antioxidant activity of some Scorzonera species. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 83(2): 271-276.
  • Eruygur, N., Ucar, E., Akpulat, H.A., Shahsavari, K., Safavi, S.M., Kahrizi, D., 2019. In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Molecular Biology Reports, 46(2): 2121-2129.
  • Harkati, B., Akkal, S., Bayat, C., Laouer, H., Franca, M.D., 2010. Secondary metabolites from Scorzonera undulata ssp. deliciosa (Guss.) Maire (Asteracae) and their antioxidant activities. Records of Natural Products, 4(3): 171-175.
  • Küpeli Akkol, E., Bahadır-Acıkara, Ö., Süntar, I., Citoğlu-Saltan, G., Keleş, H., Ergene, B., 2011. Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method. Journal of Ethnopharmacology, 137(2): 1018-1027.
  • Lendzion, K., Gornowicz, A., Bielawski, K., Bielawska, A., 2021. Phytochemical composition and biological activities of Scorzonera species. International Journal of Molecular Sciences, 22(10): 1-42.
  • Milella, L., Bader, A., De Tommasi, N., Russo, D., Braca, A., 2014. Antioxidant and free radical-scavenging activity of constituents from two Scorzonera species. Food Chemistry, 160: 298-304.
  • Molan, A.L., Mahdy, A.S., 2014. Iraqi medicinal plants: Total flavonoid contents, free-radical scavenging and bacterial beta-glucuronidase inhibition activities. IOSR Journal of Dental and Medical Sciences, 13(5): 72-77.
  • Nasseri, M.A., Bigy, S.S., Allahresani, A., Malekaneh, M., 2015. Assessment of antioxidant activity, chemical characterization and evaluation of fatty acid compositions of Scorzonera paradoxa Fisch and CA Mey. Jundishapur Journal of Natural Pharmaceutical Products, 10(4): 1-5.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(10): 1231-1237.
  • Olszowy, M., 2019. What is responsible for antioxidant properties of polyphenolic compounds from plants? Plant Physiology and Biochemistry, 144: 135-143.
  • Petkova, N., 2018. Characterization of inulin from black salsify (Scorzonera hispanica L.) for food and pharmaceutical purposes. Asian Journal of Pharmaceutical and Clinical Research, 11(12): 221-225.
  • Sarı, A., Şahin, H., Özsoy, N., Özbek Çelik, B., 2019. Phenolic compounds and in vitro antioxidant, anti-inflammatory, antimicrobial activities of Scorzonera hieraciifolia Hayek roots. South African Journal of Botany, 125: 116-119.
  • Sarı, A., Zidorn, C., Ellmerer, E.P., Özgökçe, F., Ongania, K.H., Stuppner, H., 2007. Phenolic compounds from Scorzonera tomentosa L. Helvetica Chimica Acta, 90(2): 311-317.
  • Sweidan, A., El-Mestrah, M., Kanaan, H., Dandache, I., Merhi, F., Chokr, A., 2020. Antibacterial and antibiofilm activities of Scorzonera mackmeliana. Pakistan Journal of Pharmaceutical Sciences, 33(1): 199-206.
  • Taşkın, D., Gecim, M., Doğan, A., Beceren, A., 2021. Polyphenolic composition and antioxidant effect of aerial parts and roots extracts from Scorzonera veratrifolia. International Journal of Secondary Metabolite, 8(3): 284-299.
  • Tsevegsuren, N., Edrada, R.., Lin, W., Ebel, R., Torre, C., Ortlepp, S., Wray, V., Proksch, P., 2007. Biologically active natural products from Mongolian medicinal plants scorzonera divaricata and scorzonera pseudodivaricata. Journal of Natural Products, 70(6): 962967.
  • Wang, Y., Edrada-Ebel, R.A., Tsevegsuren, N., Sendker, J., Braun, M., Wray, V., Lin, W., Proksch, P., 2009. Dihydrostilbene derivatives from the Mongolian medicinal plant Scorzonera radiata. Journal of Natural Products, 72(4): 671-675.
  • Wu, Q.-X., He, X.-F., Jiang, C.-X., Zhang, W., Shi, Z.-N., Li, H.-F., Zhu, Y., 2018. Two novel bioactive sulfated guaiane sesquiterpenoid salt alkaloids from the aerial parts of Scorzonera divaricata. Fitoterapia, 124: 113-119.
  • Yang, Y.-J., Yao, J., Jin, X.-J., Shi, Z.-N., Shen, T.-F., Fang, J.-G., Yao, X.-J., Zhu, Y., 2016. Sesquiterpenoids and tirucallane triterpenoids from the roots of Scorzonera divaricata. Phytochemistry, 124: 86-98.
There are 31 citations in total.

Details

Primary Language English
Subjects Plant Biochemistry
Journal Section Research Article
Authors

Gülşen Güçlü 0000-0002-3599-213X

Nuraniye Eruygur 0000-0002-4674-7009

Esra Uçar 0000-0001-6327-4779

Dilara Ülger Özbek 0000-0002-6834-020X

Halil Bal 0000-0002-0017-3425

Aşkın Akpolat 0000-0001-8394-2746

Danial Kahrizi 0000-0002-1717-6075

Publication Date July 31, 2023
Published in Issue Year 2023 Volume: 10 Issue: 2

Cite

APA Güçlü, G., Eruygur, N., Uçar, E., Ülger Özbek, D., et al. (2023). Biological Activity Evaluation of Scorzonera tomentosa L. Türkiye Tarımsal Araştırmalar Dergisi, 10(2), 162-167. https://doi.org/10.19159/tutad.1278795
AMA Güçlü G, Eruygur N, Uçar E, Ülger Özbek D, Bal H, Akpolat A, Kahrizi D. Biological Activity Evaluation of Scorzonera tomentosa L. TÜTAD. July 2023;10(2):162-167. doi:10.19159/tutad.1278795
Chicago Güçlü, Gülşen, Nuraniye Eruygur, Esra Uçar, Dilara Ülger Özbek, Halil Bal, Aşkın Akpolat, and Danial Kahrizi. “Biological Activity Evaluation of Scorzonera Tomentosa L”. Türkiye Tarımsal Araştırmalar Dergisi 10, no. 2 (July 2023): 162-67. https://doi.org/10.19159/tutad.1278795.
EndNote Güçlü G, Eruygur N, Uçar E, Ülger Özbek D, Bal H, Akpolat A, Kahrizi D (July 1, 2023) Biological Activity Evaluation of Scorzonera tomentosa L. Türkiye Tarımsal Araştırmalar Dergisi 10 2 162–167.
IEEE G. Güçlü, “Biological Activity Evaluation of Scorzonera tomentosa L”., TÜTAD, vol. 10, no. 2, pp. 162–167, 2023, doi: 10.19159/tutad.1278795.
ISNAD Güçlü, Gülşen et al. “Biological Activity Evaluation of Scorzonera Tomentosa L”. Türkiye Tarımsal Araştırmalar Dergisi 10/2 (July 2023), 162-167. https://doi.org/10.19159/tutad.1278795.
JAMA Güçlü G, Eruygur N, Uçar E, Ülger Özbek D, Bal H, Akpolat A, Kahrizi D. Biological Activity Evaluation of Scorzonera tomentosa L. TÜTAD. 2023;10:162–167.
MLA Güçlü, Gülşen et al. “Biological Activity Evaluation of Scorzonera Tomentosa L”. Türkiye Tarımsal Araştırmalar Dergisi, vol. 10, no. 2, 2023, pp. 162-7, doi:10.19159/tutad.1278795.
Vancouver Güçlü G, Eruygur N, Uçar E, Ülger Özbek D, Bal H, Akpolat A, Kahrizi D. Biological Activity Evaluation of Scorzonera tomentosa L. TÜTAD. 2023;10(2):162-7.

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