Research Article
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Some Free Amino Acid Contents in the Seeds from Mutant Grass Pea Genotypes

Year 2022, Volume: 9 Issue: 4, 885 - 891, 18.10.2022

Mehmet Arslan Tuğba Hasibe Gökkaya Emine Doğan Çetin Taner Erkaymaz Engin Yol

Abstract

Grass pea (Lathyrus sativus L.) is preferred by animals for both its grains, green and hay as is potential important nutrient source for animal feeding. This crop is a rich source of protein and contains amino acids. With this research, 40 genotypes, a special collection developed through the EMS mutagen, were examined for free amino acid content including aspargine, L-homoarginine, arginine, aspartic acid, glutamic acid, proline, methionine, tyrosine, leucine + isoleucine, phenylalanine. The least abundant free amino acid in grass pea seeds is proline and was found as 3.53 mg g-1. L-homoarginine gave the highest free amino acids contents was obtained at 12369.40 mg g−1. These variations can be useful and usable throughout product/variety development phase.

Keywords

Lathyrus sativus free amino acids EMS mutation breeding

Supporting Institution

Akdeniz University

Project Number

FYL-2020-5330

References

  • Aniszewski. T.. Ylinampa. T.A.. Haikonen. J.A., Pynttari. A.S. 2013. Crude protein and nitrogen-free content diversity and accelerating potential in seeds of economic legumes. Legume Research. 36(2): 165-173.
  • Arslan, M., Oten, M., Erkaymaz, T., Tongur, T., Kilic, M., Elmasulu, S., Cinar, A, 2017. β-N-oxalyl-L-2.3-diaminopropionic acid. L-homoarginine and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/M. International Journal of Food Properties, 20 (S1): 108-118.
  • Arslan. M. 2017a. Defining free amino acid contents of grass pea (Lathyrus sativus) genotypes in Turkey. Cogent Chemistry, 3: 1302311.
  • Arslan. M. 2017b. Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.). Legume Research, 40(5): 803-810.
  • Ates. E.. Coskuntuna. L., Tekeli. A.S. 2010. The amino acid and fiber contents of four different annual forage legumes at full- bloom stage. Cuban J. Agric. Sci.. 44: 73-78.
  • Bell, E.A. 2003. Non-protein amino acids of plant: significance in medicine. nutrition. and agriculture. J Agric Food Chem, 51:2854–65.
  • Carratu, B.. Boniglia, C.. Giammarioli, S. Mosca, M., Sanzini, E. 2008. Free Amino Acids in Botanicals and Botanical Preparations. Journal of Food Chemistry. 73(5): 323-327.
  • Fikre, A., Korbu, L., Kuo, Y.H., Lambein, F. 2008. The contents of the neuro-excitatory amino acid β-ODAP (b-N-oxalyl-L-a.bdiaminopropionic acid) and other free and protein amino acids in the seeds of different genotypes of grass pea (Lathyrus sativus L.). Food Chemistry, 110: 422–427.
  • Grusak, M.A. and DellaPenna, D. 1999. Improving the nutrient composition of plants to enhance human nutrition and health. Annu. Rev. Plant Physiol. Plant Mol. Biol., 50: 133–161
  • Hanbury, C.D., Siddique, K.H.M., Galwey, N.W., Cocks, P.S. 1999. Genotype-environment interaction for seed yield and ODAP concentration of Lathyrus sativus L. and L. cicera L. in Mediterranean-type environments. Euphytica, 110: 445–460.
  • Iezzoni, A. F., and Pritts, M. P. 1991. Application of principal component analysis to horticultural research. HortScience, 26, 334–338.
  • Kıvrak, İ., Kıvrak, Ş.,, Harmandar, M., 2014. Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC-MS/MS. Food Chermistry, 158: 88-92.
  • Kumar, S., Bejiga, G., Ahmed, S., Nakkoul, H., Sarker, A. 2011. Genetic improvement of grass pea for low neurotoxin (β-ODAP) content. Food and Chemical Toxicology, 49: 589-600.
  • Lambein, F.. Travella, S.. Kuo, Y.H.. Montagu, M.V., Heijde, M. 2019. Grass pea (Lathyrus sativus L.): orphan crop. nutraceutical or just plain food? Planta, 250(3) SI: 821-838.
  • Megias, C., Cortes-Giraldo, I., Giron-Calle, J., Alaiz, M., Vioque, J. 2016a. Free Amino Acids. Including Canavanine. in the Seeds from 24 Wild Mediterranean Legumes. J. Food Chem Nanotechnol, 2(4): 178-183.
  • Megia, C., Cortes-Giraldo, I., Giron-Calle, J., Alaiz, M., Vioque, J. 2016b. Free amino acids. including canavanine. in the seeds from 32 Vicia species belonging to subgenus Vicilla. Biocatalysis and Agricultural Biotechnology. 8: 126-129.
  • Nimbalkar, M.S., Pai, S.R., Pawar, N.V., Oulkar, D., Dixit. G.B. 2012. Free amino acid profiling in grain amaranth using LC-MS/MS. Food Chemistry. 134: 2565-2569.
  • Vaz Patto MC, Skiba B, Pang ECK, Ochatt SJ, Lambein F, Rubiales D (2006) Lathyrus improvement for resistance against biotic and abiotic stresses: From classical breeding to marker assisted selection. Euphytica (2006) 147: 133–147.
  • Zhao, L.; Chen, X.G.; Hu, Z.D.; Li, Q.F.; Chen, Q.; Li, Z.X. 1999. Analysis of b-N-oxalyl-L-a,b-diaminopropionic acid and homoarginine in Lathyrus sativus by capillary zone electrophoresis. Journal of Chromatography, 857, 295–302.

Year 2022, Volume: 9 Issue: 4, 885 - 891, 18.10.2022

Mehmet Arslan Tuğba Hasibe Gökkaya Emine Doğan Çetin Taner Erkaymaz Engin Yol

Abstract

Project Number

FYL-2020-5330

References

  • Aniszewski. T.. Ylinampa. T.A.. Haikonen. J.A., Pynttari. A.S. 2013. Crude protein and nitrogen-free content diversity and accelerating potential in seeds of economic legumes. Legume Research. 36(2): 165-173.
  • Arslan, M., Oten, M., Erkaymaz, T., Tongur, T., Kilic, M., Elmasulu, S., Cinar, A, 2017. β-N-oxalyl-L-2.3-diaminopropionic acid. L-homoarginine and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/M. International Journal of Food Properties, 20 (S1): 108-118.
  • Arslan. M. 2017a. Defining free amino acid contents of grass pea (Lathyrus sativus) genotypes in Turkey. Cogent Chemistry, 3: 1302311.
  • Arslan. M. 2017b. Diversity for vitamin and amino acid content in grass pea (Lathyrus sativus L.). Legume Research, 40(5): 803-810.
  • Ates. E.. Coskuntuna. L., Tekeli. A.S. 2010. The amino acid and fiber contents of four different annual forage legumes at full- bloom stage. Cuban J. Agric. Sci.. 44: 73-78.
  • Bell, E.A. 2003. Non-protein amino acids of plant: significance in medicine. nutrition. and agriculture. J Agric Food Chem, 51:2854–65.
  • Carratu, B.. Boniglia, C.. Giammarioli, S. Mosca, M., Sanzini, E. 2008. Free Amino Acids in Botanicals and Botanical Preparations. Journal of Food Chemistry. 73(5): 323-327.
  • Fikre, A., Korbu, L., Kuo, Y.H., Lambein, F. 2008. The contents of the neuro-excitatory amino acid β-ODAP (b-N-oxalyl-L-a.bdiaminopropionic acid) and other free and protein amino acids in the seeds of different genotypes of grass pea (Lathyrus sativus L.). Food Chemistry, 110: 422–427.
  • Grusak, M.A. and DellaPenna, D. 1999. Improving the nutrient composition of plants to enhance human nutrition and health. Annu. Rev. Plant Physiol. Plant Mol. Biol., 50: 133–161
  • Hanbury, C.D., Siddique, K.H.M., Galwey, N.W., Cocks, P.S. 1999. Genotype-environment interaction for seed yield and ODAP concentration of Lathyrus sativus L. and L. cicera L. in Mediterranean-type environments. Euphytica, 110: 445–460.
  • Iezzoni, A. F., and Pritts, M. P. 1991. Application of principal component analysis to horticultural research. HortScience, 26, 334–338.
  • Kıvrak, İ., Kıvrak, Ş.,, Harmandar, M., 2014. Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC-MS/MS. Food Chermistry, 158: 88-92.
  • Kumar, S., Bejiga, G., Ahmed, S., Nakkoul, H., Sarker, A. 2011. Genetic improvement of grass pea for low neurotoxin (β-ODAP) content. Food and Chemical Toxicology, 49: 589-600.
  • Lambein, F.. Travella, S.. Kuo, Y.H.. Montagu, M.V., Heijde, M. 2019. Grass pea (Lathyrus sativus L.): orphan crop. nutraceutical or just plain food? Planta, 250(3) SI: 821-838.
  • Megias, C., Cortes-Giraldo, I., Giron-Calle, J., Alaiz, M., Vioque, J. 2016a. Free Amino Acids. Including Canavanine. in the Seeds from 24 Wild Mediterranean Legumes. J. Food Chem Nanotechnol, 2(4): 178-183.
  • Megia, C., Cortes-Giraldo, I., Giron-Calle, J., Alaiz, M., Vioque, J. 2016b. Free amino acids. including canavanine. in the seeds from 32 Vicia species belonging to subgenus Vicilla. Biocatalysis and Agricultural Biotechnology. 8: 126-129.
  • Nimbalkar, M.S., Pai, S.R., Pawar, N.V., Oulkar, D., Dixit. G.B. 2012. Free amino acid profiling in grain amaranth using LC-MS/MS. Food Chemistry. 134: 2565-2569.
  • Vaz Patto MC, Skiba B, Pang ECK, Ochatt SJ, Lambein F, Rubiales D (2006) Lathyrus improvement for resistance against biotic and abiotic stresses: From classical breeding to marker assisted selection. Euphytica (2006) 147: 133–147.
  • Zhao, L.; Chen, X.G.; Hu, Z.D.; Li, Q.F.; Chen, Q.; Li, Z.X. 1999. Analysis of b-N-oxalyl-L-a,b-diaminopropionic acid and homoarginine in Lathyrus sativus by capillary zone electrophoresis. Journal of Chromatography, 857, 295–302.
There are 19 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Research Articles
Authors

Mehmet Arslan 0000-0002-2197-4969

Tuğba Hasibe Gökkaya 0000-0001-5956-0764

Emine Doğan Çetin 0000-0002-8803-5341

Taner Erkaymaz 0000-0002-1939-3153

Engin Yol 0000-0002-3152-6078

Project Number FYL-2020-5330
Publication Date October 18, 2022
Submission Date March 29, 2022
Published in Issue Year 2022 Volume: 9 Issue: 4

Cite

APA Arslan, M., Gökkaya, T. H., Doğan Çetin, E., Erkaymaz, T., et al. (2022). Some Free Amino Acid Contents in the Seeds from Mutant Grass Pea Genotypes. Türk Tarım Ve Doğa Bilimleri Dergisi, 9(4), 885-891.
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