Abstract
Aflatoxin B1 (afl B1) binding capacity of a mixed toxin binder used in poultry nutrition were determined using the central composite design technique. Experimental conditions such as pH, temperature and incubation time for the determination of the binding capacity of a mix binder were optimized for Central Composite Design. The impact of these three independent variables on the % binding of aflatoxin B1 was evaluated at different five levels (-1.68, -1, 0, 1, 1.68). The optimum experimental conditions were 5.8, 42°C, 94.11 min for pH, temperature and incubation time, respectively using quadratic model and desirability function. A significant effect of each independent variable was observed on the % binding efficiency of aflatoxin B1. In optimum experimental conditions, aflatoxin B1 binding capacity with mix toxin binder was found 97%. The results of the present study indicated that the mix binder is very suitable for binding of aflatoxin B1 and the central composite design can be used effectively in determining the optimized parameters for improving toxin binding capacity of aflatoxin B1.
References
- Additives, E. P. o., Feed, P. o. S. u. i. A., Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. d. L., Bories, G., Chesson, A., Cocconcelli, P. S. and Flachowsky, G., 2018, Guidance on the assessment of the efficacy of feed additives, EFSA Journal, 16 (5), e05274.
- Ahn, J. Y., Kim, J., Cheong, D. H., Hong, H., Jeong, J. Y. and Kim, B. G., 2022, An in vitro study on the efficacy of mycotoxin sequestering agents for aflatoxin B1, deoxynivalenol, and zearalenone, Animals, 12 (3), 333.
- Bočarov-Stančić, A., Lopičić, Z. R., Bodroža-Solarov, M. I., Stanković, S., Janković, S., Milojković, J. V. and Krulj, J. A., 2018, In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia, Food and Feed research, 45 (7), 87-96.
- Bui-Klimke, T. R. and Wu, F., 2015, Ochratoxin A and human health risk: A review of the evidence, Critical Reviews in Food Science and Nutrition, 55 (13), 1860-1869.
- Calado, T., Venâncio, A. and Abrunhosa, L., 2014, Irradiation for mold and mycotoxin control: A review, Comprehensive Reviews in Food Science and Food Safety, 13 (5), 1049-1061.
- Commission, B. P., 1998, British Pharmacopoeia London, Her Majesty's Stationary Office, p.
- De Mil, T., Devreese, M., De Baere, S., Van Ranst, E., Eeckhout, M., De Backer, P. and Croubels, S., 2015, Characterization of 27 mycotoxin binders and the relation with in vitro zearalenone adsorption at a single concentration, Toxins, 7 (1), 21-33.
- Diaz, D. E., Hagler, W. M., Hopkins, B. A. and Whitlow, L. W., 2003, Aflatoxin binders I: in vitro binding assay for aflatoxin B1 by several potential sequestering agents, Mycopathologia, 156 (3), 223-226.
- Eskola, M., Kos, G., Elliott, C. T., Hajšlová, J., Mayar, S. and Krska, R., 2020, Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited ‘FAO estimate’of 25%, Critical Reviews in Food Science and Nutrition, 60 (16), 2773-2789.
- Faucet-Marquis, V., Joannis-Cassan, C., Hadjeba-Medjdoub, K., Ballet, N. and Pfohl-Leszkowicz, A., 2014, Development of an in vitro method for the prediction of mycotoxin binding on yeast-based products: Case of aflatoxin B1, zearalenone and ochratoxin A, Applied Microbiology and Biotechnology, 98 (17), 7583-7596.
- Frizzell, C., Ndossi, D., Verhaegen, S., Dahl, E., Eriksen, G., Sørlie, M., Ropstad, E., Muller, M., Elliott, C. and Connolly, L., 2011, Endocrine disrupting effects of zearalenone, alpha-and beta-zearalenol at the level of nuclear receptor binding and steroidogenesis, Toxicology Letters, 206 (2), 210-217.
- Gomaa, M., Ayesh, A., Galil, M. A. and Naguib, K., 1997, Effect of high pressure ammoniation procedure on the detoxification of aflatoxins, Mycotoxin research, 13 (1), 23-34.
- HUB, E. S., Feed additives, https://ec.europa.eu/jrc/en/eurl/feed-additives: [18.01.2022].
- İPÇAK, H. H., ALÇİÇEK, A. and ÖZÜRETMEN, S., 2019, Evaluation of the effects of mycotoxin binders in animal nutrition, Black Sea Journal of Agriculture, 2 (4), 218-223.
- Kovač, M., Šubarić, D., Bulaić, M., Kovač, T. and Šarkanj, B., 2018, Yesterday masked, today modified; what do mycotoxins bring next?, Arhiv Za Higijenu Rada i Toksikologiju, 69 (3), 196-214.
- Lyagin, I. and Efremenko, E., 2019, Enzymes for detoxification of various mycotoxins: Origins and mechanisms of catalytic action, Molecules, 24 (13), 2362.
- Mannaa, M. and Kim, K. D., 2017, Influence of temperature and water activity on deleterious fungi and mycotoxin production during grain storage, Mycobiology, 45 (4), 240-254.
- Milani, J., 2013, Ecological conditions affecting mycotoxin production in cereals: a review, Veterinarni Medicina, 58 (8).
- Pitt, J., 2000, Toxigenic fungi: which are important?, Sabouraudia, 38 (Supplement_1), 17-22.
- Raters, M. and Matissek, R., 2008, Thermal stability of aflatoxin B 1 and ochratoxin A, Mycotoxin research, 24 (3), 130-134.
- Rushing, B. R. and Selim, M. I., 2019, Aflatoxin B1: A review on metabolism, toxicity, occurrence in food, occupational exposure, and detoxification methods, Food and Chemical Toxicology, 124, 81-100.
- Schiefer, H., 1990, Mycotoxicoses of domestic animals and their diagnosis, Canadian Journal of Physiology and Pharmacology, 68 (7), 987-990.
- Taheur, F. B., Kouidhi, B., Al Qurashi, Y. M. A., Salah-Abbès, J. B. and Chaieb, K., 2019, Biotechnology of mycotoxins detoxification using microorganisms and enzymes, Toxicon, 160, 12-22.
- Topkafa, M. and Ayyildiz, H. F., 2017, An implementation of central composite design: Effect of microwave and conventional heating techniques on the triglyceride composition and trans isomer formation in corn oil, International Journal of Food Properties, 20 (1), 198-212.
- Trombete, F., Porto, Y., Freitas‐Silva, O., Pereira, R., Direito, G., Saldanha, T. and Fraga, M., 2017, Efficacy of ozone treatment on mycotoxins and fungal reduction in artificially contaminated soft wheat grains, Journal of Food Processing and Preservation, 41 (3), e12927.
- Vekiru, E., Fruhauf, S., Rodrigues, I., Ottner, F., Krska, R., Schatzmayr, G., Ledoux, D., Rottinghaus, G. and Bermudez, A., 2015, In vitro binding assessment and in vivo efficacy of several adsorbents against aflatoxin B1, World Mycotoxin Journal, 8 (4), 477-488.
- Vila-Donat, P., Marín, S., Sanchis, V. and Ramos, A., 2018, A review of the mycotoxin adsorbing agents, with an emphasis on their multi-binding capacity, for animal feed decontamination, Food and Chemical Toxicology, 114, 246-259.
- Yalcin, N. F., Avci, T., Isik, M. K. and Oguz, H., 2018, In vitro activity of toxin binders on aflatoxin B1 in poultry gastrointestinal medium, Pakistan Veterinary Journal, 38 (1).
Aflatoksin B1 Bağlama Kapasitesinin Karışık Toksin Bağlayıcı ile Merkezi Kompozit Tasarım Kullanılarak Değerlendirilmesi
Abstract
Kanatlı beslenmesinde kullanılan karışık bir toksin bağlayıcı olan aflatoksin B1`in bağlama kapasitesi, merkezi kompozit tasarım tekniği kullanılarak belirlenmiştir. Karışım bağlayıcının bağlama kapasitesinin belirlenmesi için pH, sıcaklık ve inkübasyon süresi gibi deneysel koşulların optimizasyonu için merkezi kompozit tasarım kullanılmıştır. Bu üç bağımsız değişkenin aflatoksin B1'in bağlanma yüzdesi üzerindeki etkisi beş seviyede (-1.68, -1, 0, 1, 1.68) uygulanmıştır. Bu değişkenlerin 3 boyutlu grafikler dikkate alındığında aflatoksin B1 bağlanması üzerinde önemli etkiye sahip olduğu gözlenmiştir. Kuadratik model ve desirability fonksiyonu yardımıyla optimum deneysel koşullar pH 5.8, sıcaklık 42°C ve inkübasyon süresi 94.11 dakika olarak tespit edilmiştir. Her bir bağımsız değişken, aflatoksin B1'in bağlanma yüzdesi üzerinde anlamlı bir etkiye sahipti. Optimum deney koşullarında karışık toksin bağlayıcının aflatoksin B1'i %97 gibi yüksek bir oranında bağladığı belirlenmiştir. Sonuçlar, karışım bağlayıcının aflatoksin B1'in bağlanması için çok uygun olduğunu ve merkezi kompozit tasarımın toksin bağlayıcıların toksin bağlama kapasitesinin belirlenmesinde etkin bir şekilde kullanılabileceğini göstermiştir.
Keywords
Aflatoksin B1 Bağlama kapasitesi Merkezi kompozit tasarım (CCD) Optimizasyon Toksin bağlayıcı
References
- Additives, E. P. o., Feed, P. o. S. u. i. A., Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. d. L., Bories, G., Chesson, A., Cocconcelli, P. S. and Flachowsky, G., 2018, Guidance on the assessment of the efficacy of feed additives, EFSA Journal, 16 (5), e05274.
- Ahn, J. Y., Kim, J., Cheong, D. H., Hong, H., Jeong, J. Y. and Kim, B. G., 2022, An in vitro study on the efficacy of mycotoxin sequestering agents for aflatoxin B1, deoxynivalenol, and zearalenone, Animals, 12 (3), 333.
- Bočarov-Stančić, A., Lopičić, Z. R., Bodroža-Solarov, M. I., Stanković, S., Janković, S., Milojković, J. V. and Krulj, J. A., 2018, In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia, Food and Feed research, 45 (7), 87-96.
- Bui-Klimke, T. R. and Wu, F., 2015, Ochratoxin A and human health risk: A review of the evidence, Critical Reviews in Food Science and Nutrition, 55 (13), 1860-1869.
- Calado, T., Venâncio, A. and Abrunhosa, L., 2014, Irradiation for mold and mycotoxin control: A review, Comprehensive Reviews in Food Science and Food Safety, 13 (5), 1049-1061.
- Commission, B. P., 1998, British Pharmacopoeia London, Her Majesty's Stationary Office, p.
- De Mil, T., Devreese, M., De Baere, S., Van Ranst, E., Eeckhout, M., De Backer, P. and Croubels, S., 2015, Characterization of 27 mycotoxin binders and the relation with in vitro zearalenone adsorption at a single concentration, Toxins, 7 (1), 21-33.
- Diaz, D. E., Hagler, W. M., Hopkins, B. A. and Whitlow, L. W., 2003, Aflatoxin binders I: in vitro binding assay for aflatoxin B1 by several potential sequestering agents, Mycopathologia, 156 (3), 223-226.
- Eskola, M., Kos, G., Elliott, C. T., Hajšlová, J., Mayar, S. and Krska, R., 2020, Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited ‘FAO estimate’of 25%, Critical Reviews in Food Science and Nutrition, 60 (16), 2773-2789.
- Faucet-Marquis, V., Joannis-Cassan, C., Hadjeba-Medjdoub, K., Ballet, N. and Pfohl-Leszkowicz, A., 2014, Development of an in vitro method for the prediction of mycotoxin binding on yeast-based products: Case of aflatoxin B1, zearalenone and ochratoxin A, Applied Microbiology and Biotechnology, 98 (17), 7583-7596.
- Frizzell, C., Ndossi, D., Verhaegen, S., Dahl, E., Eriksen, G., Sørlie, M., Ropstad, E., Muller, M., Elliott, C. and Connolly, L., 2011, Endocrine disrupting effects of zearalenone, alpha-and beta-zearalenol at the level of nuclear receptor binding and steroidogenesis, Toxicology Letters, 206 (2), 210-217.
- Gomaa, M., Ayesh, A., Galil, M. A. and Naguib, K., 1997, Effect of high pressure ammoniation procedure on the detoxification of aflatoxins, Mycotoxin research, 13 (1), 23-34.
- HUB, E. S., Feed additives, https://ec.europa.eu/jrc/en/eurl/feed-additives: [18.01.2022].
- İPÇAK, H. H., ALÇİÇEK, A. and ÖZÜRETMEN, S., 2019, Evaluation of the effects of mycotoxin binders in animal nutrition, Black Sea Journal of Agriculture, 2 (4), 218-223.
- Kovač, M., Šubarić, D., Bulaić, M., Kovač, T. and Šarkanj, B., 2018, Yesterday masked, today modified; what do mycotoxins bring next?, Arhiv Za Higijenu Rada i Toksikologiju, 69 (3), 196-214.
- Lyagin, I. and Efremenko, E., 2019, Enzymes for detoxification of various mycotoxins: Origins and mechanisms of catalytic action, Molecules, 24 (13), 2362.
- Mannaa, M. and Kim, K. D., 2017, Influence of temperature and water activity on deleterious fungi and mycotoxin production during grain storage, Mycobiology, 45 (4), 240-254.
- Milani, J., 2013, Ecological conditions affecting mycotoxin production in cereals: a review, Veterinarni Medicina, 58 (8).
- Pitt, J., 2000, Toxigenic fungi: which are important?, Sabouraudia, 38 (Supplement_1), 17-22.
- Raters, M. and Matissek, R., 2008, Thermal stability of aflatoxin B 1 and ochratoxin A, Mycotoxin research, 24 (3), 130-134.
- Rushing, B. R. and Selim, M. I., 2019, Aflatoxin B1: A review on metabolism, toxicity, occurrence in food, occupational exposure, and detoxification methods, Food and Chemical Toxicology, 124, 81-100.
- Schiefer, H., 1990, Mycotoxicoses of domestic animals and their diagnosis, Canadian Journal of Physiology and Pharmacology, 68 (7), 987-990.
- Taheur, F. B., Kouidhi, B., Al Qurashi, Y. M. A., Salah-Abbès, J. B. and Chaieb, K., 2019, Biotechnology of mycotoxins detoxification using microorganisms and enzymes, Toxicon, 160, 12-22.
- Topkafa, M. and Ayyildiz, H. F., 2017, An implementation of central composite design: Effect of microwave and conventional heating techniques on the triglyceride composition and trans isomer formation in corn oil, International Journal of Food Properties, 20 (1), 198-212.
- Trombete, F., Porto, Y., Freitas‐Silva, O., Pereira, R., Direito, G., Saldanha, T. and Fraga, M., 2017, Efficacy of ozone treatment on mycotoxins and fungal reduction in artificially contaminated soft wheat grains, Journal of Food Processing and Preservation, 41 (3), e12927.
- Vekiru, E., Fruhauf, S., Rodrigues, I., Ottner, F., Krska, R., Schatzmayr, G., Ledoux, D., Rottinghaus, G. and Bermudez, A., 2015, In vitro binding assessment and in vivo efficacy of several adsorbents against aflatoxin B1, World Mycotoxin Journal, 8 (4), 477-488.
- Vila-Donat, P., Marín, S., Sanchis, V. and Ramos, A., 2018, A review of the mycotoxin adsorbing agents, with an emphasis on their multi-binding capacity, for animal feed decontamination, Food and Chemical Toxicology, 114, 246-259.
- Yalcin, N. F., Avci, T., Isik, M. K. and Oguz, H., 2018, In vitro activity of toxin binders on aflatoxin B1 in poultry gastrointestinal medium, Pakistan Veterinary Journal, 38 (1).
Details
| Primary Language | English |
|---|---|
| Subjects | Quality Assurance, Chemometrics, Traceability and Metrological Chemistry |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | April 24, 2024 |
| Submission Date | November 14, 2023 |
| Acceptance Date | February 5, 2024 |
| Published in Issue | Year 2024 Volume: 50 Issue: 1 |
Cite
Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
It has been scanned in DergiPark since 2016.
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