Research Article
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Investigation of Genotoxic Effect of Escherichia coli in Urinary Tract Epithelial Cells with Micronucleus Assay

Year 2018, Volume: 25 Issue: 3, 298 - 302, 01.09.2018
https://doi.org/10.17343/sdutfd.375078

Abstract

Objective: E. coli
is a type of bacterium that causes urinary tract infections in women. The aim
of the work was to investigate the possible use of epithelial cells from the
urinary tract in identifying infections–related DNA damage effects in women.

Methods: Epithelial cells
from urinary tract were collected from 40 E
.coli
infection and 40 control (non- infection) women and subjected to
micronucleus assay. Number of cells with micronucleus, binucleus cells were counted in two groups as
parameters for the evaluation of genome stability. All of urine samples pH, leukocyte, leukocyte esterase, nitrite
results measured with H-800
urine analyzer and FUS-200 urine sediment analyzer.

Result: E.
coli
infection and non-infection cases test results were statistically evaluated with Mann-Whitney
U and Independent Sample T test. A p-value of <0.05 was found
statistically significant for micronucleus, binucleus, and urine , leukocyte, leukocyte esterase and
nitrite
parameters. pH
results were not found statistically significant (p>0,05).







Conclusion: The
detection of
E. coli in
urinary tract epithelial cells showed that induce DNA damage.
It
can be said that patients with early antibiotics and doses, early treatment may
help to reduce the level of DNA damage.

References

  • 1-Dielubanza JE, Schaeffer JA: Urinary Tract Infections in Women. Medical Clinics of North America. 2011;95:27–41.
  • 2- Kunin MK: Urinary Tract Infections in Females. Clinical Infectious Diseases.1994;18:1-12.
  • 3- Hotchandani R, Aggarwal KK: Urinary Tract Infections in Women. Indian Journal of Clinical Practice. 2012;23(4):187-92.
  • 4- Edén SC, Jodal U, Hanson LA, Lindberg U, Åkerlund SA. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. The Lancet. 1976;308(7984):490-2.
  • 5- Taieb F, Petit C, Nougayrède PJ, Oswald E. The Enterobacterial Genotoxins: Cytolethal Distending Toxin and Colibactin. ASMScience.org/EcoSalPlus DOI:10.1128/ ecosalplus.ESP-0008-2016.
  • 6- Healy RA, Nikolayevskiy H, Patel RJ, Crawford MJ, Herzon BS. A Mechanistic Model for Colibactin-Induced Genotoxicity. J. Am. Chem. Soc. 2016;138:15563−15570.
  • 7- Lehucher-Michel MP, Amara-Mokrane YA, Devictor B, Catilina P, Botta A. Micronuclei kinetics of exfoliated urothelial cells. 1995;354:1-7.
  • 8-Papanicolaou GN. Atlas of Exsfoliative Cytology. Cambridge, Published for the Commonwealth Fund by Harvard University Press, 1963.
  • 9- Flores-Mireles LA, Walker NJ, Caparon M, Hultgren JS. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat. Rev. Microbiol. 2015;13(5): 269–84.
  • 10-Yıldırım M, Şahin İ, Küçükbayar A, Öksüz Ş, Acar S, Yavuz TM. The Validity of the Rapidly Diagnostic Test for Early Detection of Urinary Tract İnfection. Düzce Tıp Fakültesi Dergisi. 2008;3:39-42.
  • 11-Stamm EW, Hooton MT. Management of Urinary Tract Infections in Adults. N Engl J Med. 1993; 329:1328-1334.
  • 12- Özer B, Söğüt S, Duran N, Özer C, Kuvandık G, Çetin M. The diagnostic value of laboratory tests in urinary tract infections. Turkısh Microbiological Society. 2007;37(3):152-6.
  • 13- Nougayrède JP, Homburg S, Taieb F, Boury M, Brzuszkiewicz E, Gottschalk G, Buchrieser C, Hacker J, Dobrindt U, Oswald E. Escherichia coli induces DNA double-strand breaks in eukaryotic cells. Science. 2006;313(5788):848-51.
  • 14- Şekeroğlu V, Atlı-Şekeroğlu Z. Micronucleus test for determining genotoxic damage. Türk Hijyen ve Deneysel Biyoloji Dergisi. 2011; 68(4): 241 - 52.

Escherichia coli'nin Üriner Sistem Epitelyal Hücrelerinde Mikronükleus Testi ile Genotoksik Etkisinin İncelenmesi

Year 2018, Volume: 25 Issue: 3, 298 - 302, 01.09.2018
https://doi.org/10.17343/sdutfd.375078

Abstract

Amaç: E. coli, kadınlarda idrar yolu enfeksiyonlarına neden olan bir bakteri türüdür. Çalışmanın amacı, idrar yollarından epitel hücrelerinin kadınlarda enfeksiyona bağlı DNA hasar etkilerini tanımlamada olası kullanımını araştırmaktır.

Yöntem: İdrar yollarından alınan epitel hücreleri, 40 E.coli enfeksiyonu ve 40 kontrol (enfeksiyon olmayan) kadıntan toplandı ve mikronukleus testine tabi tutuldu. Mikronükleuslu hücrelerin sayısı, iki çekirdekli hücreler, genom istikrarının değerlendirilmesi için parametreler olarak iki grupta sayılmıştır. Tüm idrar örnekleri pH, lökosit, lökosit esteraz, nitrit H-800 idrar analizörü ve FUS-200 idrar tortu analizörü ile ölçüldü.

Sonuç: E. coli enfeksiyonu ve enfeksiyon olmayan olgular test sonuçları Mann-Whitney U ve Bağımsız Örnek T testi ile istatistiksel olarak değerlendirildi. Mikronükleus, binucleus ve idrar, lökosit, lökosit esteraz ve nitrit parametreleri için <0.05 anlamlı bir p-değeri bulundu. pH değerleri istatistiksel olarak anlamlı bulunmadı (p> 0,05).

Tartışma: Üriner sistem epitel hücrelerinde E. coli'nin saptanması, DNA hasarına neden olduğunu gösterdi. Erken antibiyotik ve dozlara sahip hastaların, erken tedavi, DNA hasarının seviyesini düşürmeye yardımcı olabileceği söylenebilir.

References

  • 1-Dielubanza JE, Schaeffer JA: Urinary Tract Infections in Women. Medical Clinics of North America. 2011;95:27–41.
  • 2- Kunin MK: Urinary Tract Infections in Females. Clinical Infectious Diseases.1994;18:1-12.
  • 3- Hotchandani R, Aggarwal KK: Urinary Tract Infections in Women. Indian Journal of Clinical Practice. 2012;23(4):187-92.
  • 4- Edén SC, Jodal U, Hanson LA, Lindberg U, Åkerlund SA. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. The Lancet. 1976;308(7984):490-2.
  • 5- Taieb F, Petit C, Nougayrède PJ, Oswald E. The Enterobacterial Genotoxins: Cytolethal Distending Toxin and Colibactin. ASMScience.org/EcoSalPlus DOI:10.1128/ ecosalplus.ESP-0008-2016.
  • 6- Healy RA, Nikolayevskiy H, Patel RJ, Crawford MJ, Herzon BS. A Mechanistic Model for Colibactin-Induced Genotoxicity. J. Am. Chem. Soc. 2016;138:15563−15570.
  • 7- Lehucher-Michel MP, Amara-Mokrane YA, Devictor B, Catilina P, Botta A. Micronuclei kinetics of exfoliated urothelial cells. 1995;354:1-7.
  • 8-Papanicolaou GN. Atlas of Exsfoliative Cytology. Cambridge, Published for the Commonwealth Fund by Harvard University Press, 1963.
  • 9- Flores-Mireles LA, Walker NJ, Caparon M, Hultgren JS. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat. Rev. Microbiol. 2015;13(5): 269–84.
  • 10-Yıldırım M, Şahin İ, Küçükbayar A, Öksüz Ş, Acar S, Yavuz TM. The Validity of the Rapidly Diagnostic Test for Early Detection of Urinary Tract İnfection. Düzce Tıp Fakültesi Dergisi. 2008;3:39-42.
  • 11-Stamm EW, Hooton MT. Management of Urinary Tract Infections in Adults. N Engl J Med. 1993; 329:1328-1334.
  • 12- Özer B, Söğüt S, Duran N, Özer C, Kuvandık G, Çetin M. The diagnostic value of laboratory tests in urinary tract infections. Turkısh Microbiological Society. 2007;37(3):152-6.
  • 13- Nougayrède JP, Homburg S, Taieb F, Boury M, Brzuszkiewicz E, Gottschalk G, Buchrieser C, Hacker J, Dobrindt U, Oswald E. Escherichia coli induces DNA double-strand breaks in eukaryotic cells. Science. 2006;313(5788):848-51.
  • 14- Şekeroğlu V, Atlı-Şekeroğlu Z. Micronucleus test for determining genotoxic damage. Türk Hijyen ve Deneysel Biyoloji Dergisi. 2011; 68(4): 241 - 52.
There are 14 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Okan Sancer 0000-0001-7935-5004

Publication Date September 1, 2018
Submission Date January 4, 2018
Acceptance Date March 19, 2018
Published in Issue Year 2018 Volume: 25 Issue: 3

Cite

Vancouver Sancer O. Investigation of Genotoxic Effect of Escherichia coli in Urinary Tract Epithelial Cells with Micronucleus Assay. Med J SDU. 2018;25(3):298-302.

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