THE EFFECT of SOME ANTIBIOTIC and FUNGUCIDE APPLICATIONS on the MICROPROPAGATION of Lilium candidum L.
Abstract
Lilium candidum is one of the important
commercial crop worldwide. In this study, a protocol for micropropagation of Lilium
candidum was
developed the effect of some antibiotic and fungicide applications and also the
effect of the light. In
experiments, bulb scales of Lilium candidum L. were used. The plant
material was sterilized superficially and it was dipped in solutions which contain
antibiotic and fungicide 30 minutes was cut into pieces and cultured in
Murashige and Skoog (1962) medium supplemented with 0.1 mg/L NAA +
0.01 mg/L BA, 30 mg/L sucrose and 8 mg/L agar.
The application of antibiotic and fungicide was the application of Streptomycin
+ Penicillin, Streptomycin + Benomyl and Benomyl + Nystatin in every two
different doses. Bulb
scales were cutting into lateral as 5-10 mm pieces. The explants were cultured
16 hrs light - 8 hrs dark photoperiodic condition; the light was 1600 lux, and
also were cultured in completely darkness condition in the same applications.
Culture room temperature was between 19° and 22 °C. Bulblet
formation (%) was observed in photoperiodic condition higher than darkness one.
In the Benomyl + Nystatin solution bulb formation (%) was the highest (97.4%).
In addition the best application was obtained Benomyl + Nystatin solution both
in respect of sterilization and the point of view the number of the bulblets.
Abstract
Bu
çalışmada, dünya çapında ticari öneme sahip olan Lilium candidum L.’nin
mikroçoğaltımında bazı antibiyotik ve fungusit uygulamalarının in vitro’da ki
etkisi, uygun sterilizasyon ve ışığın etkisi araştırılmıştır. Denemelerde
temel MS (1962) ortamı kullanılmış, ortama 0.1 mg l/L NAA+ 0.01 mg l/ L BA, 30
g /L şeker ve 8 g /L agar ilavesi
yapılmıştır. Bitki materyali (soğan pulları) yüzeysel sterilizasyonu takiben
antibiyotik ve fungusit içeren solusyonlarda 30 dk bekletildikten sonra
parçalara ayrılarak kültüre alınmıştır. Antibiyotik ve fungusit uygulamaları:
iki farklı dozda Streptomycin + Penicilin uygulamaları, iki farklı dozda
Streptomycin + Benomyl uygulamaları ve iki farklı dozda Benomyl + Nystatin
uygulamalarıdır. Lilium candidum soğan pulları 5-10 mm boyutunda enine parçalara
ayrılarak kültüre alınmıştır. Karanlık ve fotoperiyodik koşulda (16 saat
aydınlık- 8 saat karanlık) kültüre alınan eksplantlarda % soğancık oluşumunun,
fotoperiyodik koşulda karanlığa göre daha yüksek olduğu gözlenmiştir. Ayrıca
yüzeysel sterilizasyondan sonra yapılan uygulamalarda hem sterilizasyon
yönünden hem de eksplant başına elde edilen soğancık sayısı bakımından en
olumlu uygulamanın Benomyl + Nystatin solusyonlarının olduğu belirlenmiştir.
Keywords
Details
| Subjects | Engineering |
|---|---|
| Journal Section | Biology, Molecular Biology and Genetics |
| Authors | |
| Publication Date | June 19, 2017 |
| Published in Issue | Year 2017 Volume: 3 Issue: 1 |
Cite
Cited By
Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR
International Journal of Secondary Metabolite
https://doi.org/10.21448/ijsm.753053
Bulb Induction on Madonna Lily Stems and Three Years Growing Performance of the Bulblets
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
Dursun KURT
https://doi.org/10.18016/ksutarimdoga.vi.918548
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