Research Article
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Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus

Year 2023, Volume: 13 Issue: 2, 133 - 139, 22.12.2023

İrem Çelik Meltem Aşan Özüsağlam

https://doi.org/10.53518/mjavl.1327293

Abstract

The enhancement population of the world every day brings with it the demand for protein needs. In our study, it was aimed to identify the utilization potential of red pitahaya fruit obtained from Turkey as a natural feed additive. The disc diffusion assay, micro-dilution method for determination of minimum inhibitory (MIC) and minimum bactericidal (MBC) concentration values of the extracts were used to identify the biological activity of the extracts from red pitahaya. The red pitahaya pulp methanol extract against L. garvieae and V. alginolyticus showed 10.61 mm and 7.65 mm of inhibition zone diameters. The methanol extract of red pitahaya peel has 10.18 mm and 11.25 mm inhibition zone diameters on L. garvieae and V. alginolyticus. MIC values were determined as 20 µg/µl and 40 µg/µl for pulp methanol extract and as 80 µg/µl and 40 µg/µl for peel methanol extract against L. garvieae and V. alginolyticus. MBC values of pulp and peel methanol extracts against fish pathogens were determined as 80 µg/µl. As a result, the red pitahaya extracts may have usage potential as natural antimicrobial agents or feed additive in aquaculture.

Keywords

Hylocereus polyrhizus Fish pathogens Antimicrobial activity Natural additive

References

  • Amalia, S., Wahdaningsih, S., & Untari, E. K. (2014). Antibacterial activity testing of n-hexane fraction of red dragon (Hylocereus polyrhizus britton & rose) fruit peel on Staphylococcus aureus ATCC 25923. Majalah Obat Tradisional, 19(2), 89-94. https://doi.org/10.22146/tradmedj.8146
  • Assefa, A., & Abunna, F. (2018). Maintenance of fish health in aquaculture: review of epidemiological approaches for prevention and control of infectious disease of fish. Veterinary Medicine International. 2018, Article ID 5432497. https://doi.org/10.1155/2018/5432497
  • Balebona, M.C., Andreu, M.J., Bordas, M.A., Zorrilla, I., Moriñigo, M.A., & Borrego, J.J. (1998). Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.). Applied and Environmental Microbiology, 64 (11), 4269-4275. https://doi.org/10.1128/AEM.64.11.4269-4275.1998
  • Bilgüven, M., & Gökhan, C. (2018). Balık yemlerinde balık unu yerine tavuk ununun kullanılma olanakları. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 32 (2), 189-197.
  • Bondad‐Reantaso, M.G., MacKinnon, B., Karunasagar, I., Fridman, S., Alday‐Sanz, V., Brun, E., & Caputo, A. (2023). Review of alternatives to antibiotic use in aquaculture. Reviews in Aquaculture. https://doi.org/10.1111/raq.12786
  • Cabello, F.C. (2006). Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environmental Microbiology, 8 (7), 1137–1144. https://doi.org/10.1111/j.1462-2920.2006.01054.x
  • Cai, J., Han, H., Song, Z., Li, C., & Zhou, J. (2006). Isolation and characterization of pathogenic Vibrio alginolyticus from diseased postlarval abalone, Haliotis diversicolor supertexta (Lischke). Aquaculture Research, 37 (12), 1222-1226. https://doi.org/10.1111/j.1365-2109.2006.01552.x
  • Celik, I., & Asan-Ozusaglam, M. (2022). Investigation of Alternative Use of White Pitahaya: Natural Feed Additive in Aquaculture. 5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022) November 23-25, 2022 (p. 612-616), Ankara.
  • Chandrasekaran, M., & Venkatesalu, V. (2004). Antibacterial and antifungal activity of Syzygium jambolanum seeds. Journal of ethnopharmacology, 91(1), 105-108. https://doi.org/10.1016/j.jep.2003.12.012
  • Chen, F.R., Liu, P.C., & Lee, K.K. (2000). Lethal attribute of serine protease secreted by Vibrio alginolyticus strains in kuruma prawn. Penaeus japonicus, Zeitschrift für Naturforschung C, 55 (1-2), 94-99. https://doi.org/10.1515/znc-2000-1-218
  • Fidrianny, I., Ilham, N., & Hartati, R. (2017). Antioxidant profile and phytochemical content of different parts of super red dragon fruit (Hylocereus costaricensis) collected from West Java-Indonesia. Asian Journal of Pharmaceutical and Clinical Research, 10 (12), 290–294. http://dx.doi.org/10.22159/ajpcr.2017.v10i12.21571
  • Frans, I., Michiels, C. W., Bossier, P., Willems, K. A., Lievens, B., & Rediers, H. (2011). Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. Journal of Fish Diseases, 34(9), 643-661. https://doi.org/10.1111/j.1365-2761.2011.01279.x
  • Godfray, H.C.J., Beddington, J.R., Crute, I.R., Haddad, L., Lawrence, D., Muir, J.F., Robinson, S., Thomas, S.M., & Toulmin, C. (2010). Food security: the challenge of feeding 9 billion people. Science, 327 (5967), 812-818. https://doi.org/10.1126/science.1185383
  • Gunasena, H. P., Pushpakumara, D. K. N. G. ve Kariawasam, M., (2007). Dragon fruit, Underutilized Fruit Trees Sri lanka, 1, 110–140.
  • Hassan, N. L., Kam, K. A. R., & Zain, N. A. M. (2020). Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. International Journal of Life Sciences and Biotechnology, 3(3), 291-307. https://doi.org/10.38001/ijlsb.712542
  • Hendra, R., Masdeatresa, L., Abdulah, R., & Haryani, Y. (2019). Antibacterial activity of red dragon peel (Hylocereus polyrhizus) pigment. In Journal of Physics: Conference Series (Vol. 1351, No. 1, p. 012042). IOP Publishing. DOI 10.1088/1742-6596/1351/1/012042
  • Kocatepe, D., & Turan, H. (2018). Balık Yağları, DHA, EPA ve Sağlık. Türkiye Klinikleri J Public Health-Special Topics, 4 (1), 62-7.
  • Kowalska-Krochmal B., & Dudek-Wicher R. (2021). The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance. Pathogens, 10 (2), 165-186. https://doi.org/10.3390/pathogens10020165
  • Mann, CM., & Markham, JL. (1998). A new method for determining the minimum inhibitory concentration of essential oils. Journal of Applied Microbiology, 84 (4), 538-544. https://doi.org/10.1046/j.1365-2672.1998.00379.x
  • Meyburgh, C.M., Bragg, R.R., & Boucher, C.E. (2017). Lactococcus garvieae: An emerging bacterial pathogen of fish. Diseases of Aquatic Organisms, 123 (1), 67-79. https://doi.org/10.3354/dao03083
  • Murray, P. R., Baron, E. J., Jorgensen, J. H., Landry, M. L., & Pfaller, M. A. (2006). Manual of Clinical Microbiology: Volume 2 (No. Ed. 9). ASM press.
  • Nishikito, D.F., Borges, A.C.A., Laurindo, L.F., Otoboni, A.M.B., Direito, R., Goulart, R.D.A., Nicolau, C.C.T., Fiorini, A.M.R., Sinatora, R.V., & Barbalho, S.M. (2023). Anti-Inflammatory, Antioxidant, and Other Health Effects of Dragon Fruit and Potential Delivery Systems for Its Bioactive Compounds. Pharmaceutics, 15 (1), 159. https://doi.org/10.3390/pharmaceutics15010159
  • Nizamlıoğlu, N. M., Ünver, A., & Kadakal, Ç. (2021). Mineral content of pitaya (Hylocereus polyrhizus and Hylocereus undatus) seeds grown in Turkey. Erwerbs-Obstbau, 63, 209-213. https://doi.org/10.1007/s10341- 021-00561-x
  • Panisson, D., Marques, N. K., de Souza, F. B. M., Neto, J. C. M., Freire, A. I., & de Araújo, N. O. (2021). Growth and initial development of pitaya white (Hylocereus undatus) and red (Hylocereus monacanthus) in the city of Araguaína-TO. Research, Society and Development, 10, 14.
  • Quesada, S.P., Paschoal, J.A.R., & Reyes, F.G.R. (2013). Considerations on the aquaculture development and on the use of veterinary drugs: special issue for fluoroquinolones—a review. Journal of Food Science, 78 (9), R1321- R1333. https://doi.org/10.1111/1750-3841.12222
  • Ramli, N.S., Brown, L., Ismail, P., & Rahmat, A. (2014). Effects of red pitaya juice supplementation on cardiovascular and hepatic changes in high-carbohydrate, high-fat diet-induced metabolic syndrome rats. BMC Complementary and Alternative Medicine, 14 (1), 1-10. http://www.biomedcentral.com/1472-6882/14/189
  • Reverter, M., Bontemps, N., Lecchini, D., Banaigs, B., & Sasal, P. (2014). Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture, 433, 50-61. https://doi.org/10.1016/j.aquaculture.2014.05.048
  • Romero, J., Feijoó, C.G., & Navarrete, P. (2012). Antibiotics in aquaculture use, abuse and alternatives. Health Environ Aquacult, 159, 159-198.
  • Salikan, N. A., ZAİN, N. A. M., & Kam, K. A. R. (2020). Isolation of Antibiotic Resistant Bacteria from Rivers in Terengganu, Malaysia. International Journal of Life Sciences and Biotechnology, 3(3), 241-257. https://doi.org/10.38001/ijlsb.711948
  • Sanches-Fernandes, G. M., Sá-Correia, I., & Costa, R. (2022). Vibriosis outbreaks in aquaculture: addressing environmental and public health concerns and preventive therapies using gilthead seabream farming as a model system. Frontiers in Microbiology, 13, 904815. https://doi.org/10.3389/fmicb.2022.904815
  • Tahera, J., Feroz, F., Senjuti, J.D., Das, K.K., & Noor, R. (2014). Demonstration of anti-bacterial activity of commonly available fruit extracts in Dhaka, Bangladesh. American Journal of Microbiological Research, 2 (2), 68-73. DOI:10.12691/ajmr-2-2-5

Year 2023, Volume: 13 Issue: 2, 133 - 139, 22.12.2023

İrem Çelik Meltem Aşan Özüsağlam

https://doi.org/10.53518/mjavl.1327293

Abstract

References

  • Amalia, S., Wahdaningsih, S., & Untari, E. K. (2014). Antibacterial activity testing of n-hexane fraction of red dragon (Hylocereus polyrhizus britton & rose) fruit peel on Staphylococcus aureus ATCC 25923. Majalah Obat Tradisional, 19(2), 89-94. https://doi.org/10.22146/tradmedj.8146
  • Assefa, A., & Abunna, F. (2018). Maintenance of fish health in aquaculture: review of epidemiological approaches for prevention and control of infectious disease of fish. Veterinary Medicine International. 2018, Article ID 5432497. https://doi.org/10.1155/2018/5432497
  • Balebona, M.C., Andreu, M.J., Bordas, M.A., Zorrilla, I., Moriñigo, M.A., & Borrego, J.J. (1998). Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.). Applied and Environmental Microbiology, 64 (11), 4269-4275. https://doi.org/10.1128/AEM.64.11.4269-4275.1998
  • Bilgüven, M., & Gökhan, C. (2018). Balık yemlerinde balık unu yerine tavuk ununun kullanılma olanakları. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 32 (2), 189-197.
  • Bondad‐Reantaso, M.G., MacKinnon, B., Karunasagar, I., Fridman, S., Alday‐Sanz, V., Brun, E., & Caputo, A. (2023). Review of alternatives to antibiotic use in aquaculture. Reviews in Aquaculture. https://doi.org/10.1111/raq.12786
  • Cabello, F.C. (2006). Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environmental Microbiology, 8 (7), 1137–1144. https://doi.org/10.1111/j.1462-2920.2006.01054.x
  • Cai, J., Han, H., Song, Z., Li, C., & Zhou, J. (2006). Isolation and characterization of pathogenic Vibrio alginolyticus from diseased postlarval abalone, Haliotis diversicolor supertexta (Lischke). Aquaculture Research, 37 (12), 1222-1226. https://doi.org/10.1111/j.1365-2109.2006.01552.x
  • Celik, I., & Asan-Ozusaglam, M. (2022). Investigation of Alternative Use of White Pitahaya: Natural Feed Additive in Aquaculture. 5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022) November 23-25, 2022 (p. 612-616), Ankara.
  • Chandrasekaran, M., & Venkatesalu, V. (2004). Antibacterial and antifungal activity of Syzygium jambolanum seeds. Journal of ethnopharmacology, 91(1), 105-108. https://doi.org/10.1016/j.jep.2003.12.012
  • Chen, F.R., Liu, P.C., & Lee, K.K. (2000). Lethal attribute of serine protease secreted by Vibrio alginolyticus strains in kuruma prawn. Penaeus japonicus, Zeitschrift für Naturforschung C, 55 (1-2), 94-99. https://doi.org/10.1515/znc-2000-1-218
  • Fidrianny, I., Ilham, N., & Hartati, R. (2017). Antioxidant profile and phytochemical content of different parts of super red dragon fruit (Hylocereus costaricensis) collected from West Java-Indonesia. Asian Journal of Pharmaceutical and Clinical Research, 10 (12), 290–294. http://dx.doi.org/10.22159/ajpcr.2017.v10i12.21571
  • Frans, I., Michiels, C. W., Bossier, P., Willems, K. A., Lievens, B., & Rediers, H. (2011). Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. Journal of Fish Diseases, 34(9), 643-661. https://doi.org/10.1111/j.1365-2761.2011.01279.x
  • Godfray, H.C.J., Beddington, J.R., Crute, I.R., Haddad, L., Lawrence, D., Muir, J.F., Robinson, S., Thomas, S.M., & Toulmin, C. (2010). Food security: the challenge of feeding 9 billion people. Science, 327 (5967), 812-818. https://doi.org/10.1126/science.1185383
  • Gunasena, H. P., Pushpakumara, D. K. N. G. ve Kariawasam, M., (2007). Dragon fruit, Underutilized Fruit Trees Sri lanka, 1, 110–140.
  • Hassan, N. L., Kam, K. A. R., & Zain, N. A. M. (2020). Isolation of Antibiotic Resistant Bacteria from Rivers in Kelantan, Malaysia. International Journal of Life Sciences and Biotechnology, 3(3), 291-307. https://doi.org/10.38001/ijlsb.712542
  • Hendra, R., Masdeatresa, L., Abdulah, R., & Haryani, Y. (2019). Antibacterial activity of red dragon peel (Hylocereus polyrhizus) pigment. In Journal of Physics: Conference Series (Vol. 1351, No. 1, p. 012042). IOP Publishing. DOI 10.1088/1742-6596/1351/1/012042
  • Kocatepe, D., & Turan, H. (2018). Balık Yağları, DHA, EPA ve Sağlık. Türkiye Klinikleri J Public Health-Special Topics, 4 (1), 62-7.
  • Kowalska-Krochmal B., & Dudek-Wicher R. (2021). The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance. Pathogens, 10 (2), 165-186. https://doi.org/10.3390/pathogens10020165
  • Mann, CM., & Markham, JL. (1998). A new method for determining the minimum inhibitory concentration of essential oils. Journal of Applied Microbiology, 84 (4), 538-544. https://doi.org/10.1046/j.1365-2672.1998.00379.x
  • Meyburgh, C.M., Bragg, R.R., & Boucher, C.E. (2017). Lactococcus garvieae: An emerging bacterial pathogen of fish. Diseases of Aquatic Organisms, 123 (1), 67-79. https://doi.org/10.3354/dao03083
  • Murray, P. R., Baron, E. J., Jorgensen, J. H., Landry, M. L., & Pfaller, M. A. (2006). Manual of Clinical Microbiology: Volume 2 (No. Ed. 9). ASM press.
  • Nishikito, D.F., Borges, A.C.A., Laurindo, L.F., Otoboni, A.M.B., Direito, R., Goulart, R.D.A., Nicolau, C.C.T., Fiorini, A.M.R., Sinatora, R.V., & Barbalho, S.M. (2023). Anti-Inflammatory, Antioxidant, and Other Health Effects of Dragon Fruit and Potential Delivery Systems for Its Bioactive Compounds. Pharmaceutics, 15 (1), 159. https://doi.org/10.3390/pharmaceutics15010159
  • Nizamlıoğlu, N. M., Ünver, A., & Kadakal, Ç. (2021). Mineral content of pitaya (Hylocereus polyrhizus and Hylocereus undatus) seeds grown in Turkey. Erwerbs-Obstbau, 63, 209-213. https://doi.org/10.1007/s10341- 021-00561-x
  • Panisson, D., Marques, N. K., de Souza, F. B. M., Neto, J. C. M., Freire, A. I., & de Araújo, N. O. (2021). Growth and initial development of pitaya white (Hylocereus undatus) and red (Hylocereus monacanthus) in the city of Araguaína-TO. Research, Society and Development, 10, 14.
  • Quesada, S.P., Paschoal, J.A.R., & Reyes, F.G.R. (2013). Considerations on the aquaculture development and on the use of veterinary drugs: special issue for fluoroquinolones—a review. Journal of Food Science, 78 (9), R1321- R1333. https://doi.org/10.1111/1750-3841.12222
  • Ramli, N.S., Brown, L., Ismail, P., & Rahmat, A. (2014). Effects of red pitaya juice supplementation on cardiovascular and hepatic changes in high-carbohydrate, high-fat diet-induced metabolic syndrome rats. BMC Complementary and Alternative Medicine, 14 (1), 1-10. http://www.biomedcentral.com/1472-6882/14/189
  • Reverter, M., Bontemps, N., Lecchini, D., Banaigs, B., & Sasal, P. (2014). Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture, 433, 50-61. https://doi.org/10.1016/j.aquaculture.2014.05.048
  • Romero, J., Feijoó, C.G., & Navarrete, P. (2012). Antibiotics in aquaculture use, abuse and alternatives. Health Environ Aquacult, 159, 159-198.
  • Salikan, N. A., ZAİN, N. A. M., & Kam, K. A. R. (2020). Isolation of Antibiotic Resistant Bacteria from Rivers in Terengganu, Malaysia. International Journal of Life Sciences and Biotechnology, 3(3), 241-257. https://doi.org/10.38001/ijlsb.711948
  • Sanches-Fernandes, G. M., Sá-Correia, I., & Costa, R. (2022). Vibriosis outbreaks in aquaculture: addressing environmental and public health concerns and preventive therapies using gilthead seabream farming as a model system. Frontiers in Microbiology, 13, 904815. https://doi.org/10.3389/fmicb.2022.904815
  • Tahera, J., Feroz, F., Senjuti, J.D., Das, K.K., & Noor, R. (2014). Demonstration of anti-bacterial activity of commonly available fruit extracts in Dhaka, Bangladesh. American Journal of Microbiological Research, 2 (2), 68-73. DOI:10.12691/ajmr-2-2-5
There are 31 citations in total.

Details

Primary Language English
Subjects Microbiology (Other)
Journal Section Research Article
Authors

İrem Çelik 0000-0002-4689-5802

Meltem Aşan Özüsağlam 0000-0002-3638-1306

Publication Date December 22, 2023
Submission Date July 14, 2023
Published in Issue Year 2023 Volume: 13 Issue: 2

Cite

APA Çelik, İ., & Aşan Özüsağlam, M. (2023). Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus. Manas Journal of Agriculture Veterinary and Life Sciences, 13(2), 133-139. https://doi.org/10.53518/mjavl.1327293
AMA Çelik İ, Aşan Özüsağlam M. Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus. MJAVL. December 2023;13(2):133-139. doi:10.53518/mjavl.1327293
Chicago Çelik, İrem, and Meltem Aşan Özüsağlam. “Biological Activity of Red Pitahaya Extracts on Lactococcus Garvieae and Vibrio Alginolyticus”. Manas Journal of Agriculture Veterinary and Life Sciences 13, no. 2 (December 2023): 133-39. https://doi.org/10.53518/mjavl.1327293.
EndNote Çelik İ, Aşan Özüsağlam M (December 1, 2023) Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus. Manas Journal of Agriculture Veterinary and Life Sciences 13 2 133–139.
IEEE İ. Çelik and M. Aşan Özüsağlam, “Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus”, MJAVL, vol. 13, no. 2, pp. 133–139, 2023, doi: 10.53518/mjavl.1327293.
ISNAD Çelik, İrem - Aşan Özüsağlam, Meltem. “Biological Activity of Red Pitahaya Extracts on Lactococcus Garvieae and Vibrio Alginolyticus”. Manas Journal of Agriculture Veterinary and Life Sciences 13/2 (December 2023), 133-139. https://doi.org/10.53518/mjavl.1327293.
JAMA Çelik İ, Aşan Özüsağlam M. Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus. MJAVL. 2023;13:133–139.
MLA Çelik, İrem and Meltem Aşan Özüsağlam. “Biological Activity of Red Pitahaya Extracts on Lactococcus Garvieae and Vibrio Alginolyticus”. Manas Journal of Agriculture Veterinary and Life Sciences, vol. 13, no. 2, 2023, pp. 133-9, doi:10.53518/mjavl.1327293.
Vancouver Çelik İ, Aşan Özüsağlam M. Biological Activity of Red Pitahaya Extracts on Lactococcus garvieae and Vibrio alginolyticus. MJAVL. 2023;13(2):133-9.
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