Protective and Therapeutic Effects of Polygonum cognatum Meissn Aqueous Extract in Experimental Colitis
Abstract
The aim of the study was to investigate the protective and
therapeutic effects of Polygonum cognatum Meissn (PC) in
the treatment of colitis. Rats were orogastrically treated with
PC (2g/kg) throughout a 10-day interval before and/or after
colitis, which was induced by intracolonic administration of
acetic acid. Microscopic damage scores and scanning electron
microscopy changes of colon segments were analyzed. The
levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha
(TNF-α) in serum, malonyldialdehyde (MDA), glutathione
(GSH), tissue factor (TF) levels, myeloperoxidase (MPO),
superoxide dismutase (SOD), catalase (CAT) and
gammaglutamyltransferase (GGT) activities in the colon
tissues were measured. PC treatment ameliorated the acetic
acid-induced disturbances in colonic tissue. Serum IL-6 and
TNF-α levels decreased with PC treated colitis group.
Colonic tissue GGT, MPO activities and MDA levels were
decreased on the other hand SOD, CAT activities and GSH
levels were increased in PC treated colitis group. PC was
effective in the treatment of acetic acid-induced colitis in
rats. This may be due to the supression and down regulation
of mediators in intestinal inflammatory response and the
expression of antioxidant and anti-inflammatory response.
In conclusion, PC can play an protective role by
downregulating the colonic tissue damage and oxidative
stress which may be related to modulating the activities of
antioxidant molecules in rat model of colitis.
Keywords: Polygonum cognatum Meissn, Oxidative Stress,
Colitis, Antioxidant, Anti-inflammatory
References
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- Halliwell B. Free radicals and antioxidants: a personal view. Nutr Rev 1994; 52:253-65.
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Year 2014,
Volume: 18 Issue: 3, 126 - 134, 22.09.2014
Abstract
References
- Myers BS, Martin JS, Dempsey DT, Parkman HP, Thomas RM, Ryan JP. Acute experimental colitis decreases colonic circular smooth muscle contractility in rats. Am J Physiol 1997; 273:928-36.
- Babbs CF. Oxygen radicals in ulcerative colitis. Free Radic Biol Med 1992; 13:169-81.
- Fabia R, Willen R, ArRajab A, Andersson R, Ahren B, Bengmark S. Acetic acid-induced colitis in the rat: a reproducible experimental model for acute ulcerative colitis. Eur Surg Res 1992; 24:211-25.
- El-Benna J, Dang PM, Gougerot-Pocidalo MA, Elbim C. Phagocyte NADPH oxidase: a multicomponent enzyme essential for host defenses. Arch Immunol Ther Exp (Warsz) 2005; 53:199-206.
- Klebanoff SJ.Myeloperoxidase: friend and foe. J Leukoc Biol 2005; 77: 598-625.
- Auphan N, DiDonato JA, Rosette C, Helmberg A, Karin M. Immunosuppression by glucocorticoids: inhibition of N NF-κB activity through induction of IkB synthesis. Science 1995; 270:286-90.
- Joshi R, Kumar S, Unnikrishnan M, Mukherjee T.Free radical scavenging reactions of sulfasalazine, 5-aminosalicylic acid and sulfapyridine: mechanistic aspects and antioxidant activity. Free Radic Res 2005; 39:1163-72.
- Ciulei J.Methodology for analysis of vegetable drugs: Practical manual on the industrial utilization of medicinal and aromatic plants, Bucharest office of the joint UNIDO 1985; Romania, 66-81.
- Gue M, Bonbonne C, Fioramonti J, More J, Del Rio-Lacheze C, Comera C, Bueno L. Stress-induced enhancement of colitis in rats: CRF and arginine vasopressin are not involved. Am J Physiol 1997; 272:84-91.
- Cevik O, Oba R, Macit C, Cetinel S, Cilingir-Kaya OT, Sener E, Sener G. Lycopene inhibits caspase-3 activity and reduces oxidative organ damage in a rat model of thermal injury. Burns 2012; 38:861-71.
- Tavukçu HH, Sener TE, Tinay I, Akbal C, Erşahin M, Cevik O, Cadirci S, Reiter RJ, Sener G. Melatonin and tadalafil treatment improves erectile dysfunction after spinal cord injury in rats. Clin Exp Pharmacol Physiol 2014; 41:309-16.
- Cevik O, Cadirci S, Sener TE, Tinay I, Akbal C, Tavukcu HH, Cetinel S, Kiran D, Sener G. Quercetin treatment against ischemia/ reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress. Free Radic Res 2013; 47:683-91.
- Szasz G. A kinetic photometric method for serum g-glutamyl transpeptidase. Clin Chem 1969; 15:124–35.
- Ingram GI, Hills M. Reference method for the one-stage prothrombintime test on human blood. J Thromb Haemost 1976; 36:237-8.
- Nieto N, Torres MI, Fernandez MI, Giron MD, Rios A, Suarez MD, Gil A. Experimental ulcerative colitis impairs antioxidant defense system in rat intestine. Dig Dis Sci 2000; 45:1820-7.
- Cetinel S, Hancioglu S, Sener E, Uner C, Kilic M, Sener G, Yegen BC. Oxytocin treatment alleviates stress-aggravated colitis by a receptor-dependent mechanism. Regul Pept 2010; 160:146-52.
- OShea JJ, Murray PJ. Cytokine signaling modules in inflammatory responses. Immunity 2008; 28:477-87.
- Emekli-Alturfan E, Yarat A, Tunalı-Akbay T, Isik F, Yenidogan G, Sener G, Sehirli O, Pisiriciler R, Ak E, Altintas A. Effect of black cumin (nigella stiva) seed oil on gastric tissue in experimental colitis. Adv Environ Bio 2011; 5:483-90.
- Halliwell B. Free radicals and antioxidants: a personal view. Nutr Rev 1994; 52:253-65.
- McKenzie SJ, Baker MS, Buffington GD, Doe WF. Evidence of oxidant-induced injury to epithelial cells during inflammatory bowel disease. J Clin Invest 1996; 98:136-41.
- Oliveira FJ, Cecchini R. Oxidative stress of liver in hamsters infected with Leishmania (L) chagasi. J Parasitol 2000; 86:1067-72.
There are 21 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | September 22, 2014 |
| Published in Issue | Year 2014 Volume: 18 Issue: 3 |