The Effect Of Alternative Industrial Wastes Used As Wetting Agents On Mycelial Growth And Ligninolytic Enzyme Activities Of Some Macrofungi
Abstract
In this study, the possible using of
different type of liquid wastes from industrial production processes as wetting
agent in macrofungi production was investigated. The effects of wetting agents
on mycelial growth and ligninolytic enzyme activities of five different
macrofungi species were determined. Pleurotus cornucopiae, Flammulina
velutipes, Panus neostrigosus, Schizophyllum commune and Ganoderma lucidum cultures were
obtained from Macrofungi Culture Collection of Department of Biology, Eskişehir
Osmangazi University (OBCC). In order to determine the best mycelial growth and
enzyme production conditions, 4 different compost materials (oak, poplar, rice,
wheat) were mixed in pairs for 10 grams and were moistened by 70% with 4
different wetting agents (whey, olive land water, artificial textile dye waste
water, molasses) in single and mixed form. Distilled water was used as positive
control. The pH of each compost sample was adjusted by adding 1% gypsum and
lime. The 6 mm diameter mycelial discs from pure fungal cultures were placed in
the center of petri plates containing compost mixtures and incubated at 28°C. It was determined that all fungi isolates produced different levels of
all ligninolytic enzymes used in the study. The highest manganese peroxidase
(MnP) activity was determined as 103.64 u/L in Pleurotus cornucopiae. On the other hand, the highest laccase and
manganese-independent/versatile peroxidase
(VP) activity was determined as 118.52 and 66.86 u/L in Ganoderma lucidum, respectively. The highest daily mycelial growth
rate and mycelial growth were determined in Schizophyllum
commune.
Keywords
References
- Bayburt, C., Karaduman, A., Çelik, U. ve Yamaç, M. (2014). Farklı gelişim dönemlerinde Pleurotus ostreatus kompostundan ligninolitik enzim ekstraksiyonu için uygun yöntem seçimi. Mantar Dergisi, 5(2), 17 24.
- Boyle, C. D., Kropp, B. R. and Reid, I. D. (1992). Solubilization and mineralization of lignin by white rot fungi. Appl. Environ. Microbiol., 58(10), 3217-3224.
- Dönmez, G. (2002). Bioaccumulation of the reactive textile dyes by Candida tropicalis growing in molasses medium. Enzyme and Microbial Technol., 30(3), 363 366.
- Elisashvili, V., Kachlishvili, E. and Penninckx, M. (2008). Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes. J. Ind. Microbiol. Biotechnol., 35(11), 1531-1538.
- Kalmıs, E., Azbar, N., Yıldız, H. and Kalyoncu, F., (2008). Feasibility of using olive mill effluent (OME) as a wetting agent during the cultivation of oyster mushroom, Pleurotus ostreatus, on wheat straw. Bioresource Technol., 99 164–169.
- Koutrotsios, G., Larou, E., Mountzouris, K.C. and Zervakis, G.I. (2016). Detoxification of Olive Mill Wastewater and Bioconversion of Olive Crop Residues into High-Value-Added Biomass by the Choice Edible Mushroom Hericium erinaceus. Appl. Biochem. Biotechnol., 180,195–209.
- Leonowicz, A., Matuszewska, A., Luterek, J., Ziegenhagen, D., Wojtaś-Wasilewska, M., Cho, N. S. and Rogalski, J. (1999). Biodegradation of lignin by white rot fungi. Fungal Gen. Biol., 27(23), 175-185.
- Loss, E., Royer, A.R., Barreto-Rodrigues, M. and Barana, A.C., (2009). Use of maize wastewater for the cultivation of the Pleurotus spp. Mushroom and optimization of its biological efficiency. J. Hazard. Mat., 166, 1522–1525.
- Maciel, M. J. M. and Ribeiro, H. C. T. (2010). Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Electronic J Biotechnol., 13(6), 14-15.
- Martirani, L., Giardina, P., Marzullo, L. and Sannia, G. (1996). Reduction of phenol content and toxicity in olive oil mill waste waters with the ligninolytic fungus Pleurotus ostreatus. Water Res., 30(8), 1914-1918.
- Mohammad, N., Alam, M. Z., Kabbashi, N. A. and Ahsan, A. (2012). Effective composting of oil palm industrial waste by filamentous fungi: A review. Res. Conserv. Recycling, 58, 69-78.
- Naraian, R., Srivastava, J. and Garg, S.K. (2011). Influence of dairy spent wash (DSW) on different cultivation phases and yield response of two Pleurotus mushrooms. Ann. Microbiol., 61,853–862.
- Öztürk, A. ve Çopur, Ö. U. (2009). Mantar bileşenlerinin teröpatik etkileri. Bahçe, 38(1), 19-24.
- Pekşen, A. ve Günay, A. (2009). Kültür Mantarı (Agaricus bisporus (L.) Sing.) Yetiştiriciliğinde Çay Atığı ve Buğday Sapı Karışımından Hazırlanan Kompostların Kullanımı. Ekoloji Dergisi, 19(73), 48-54.
- Roupas, P., Keogh, J., Noakes, M., Margetts, C. and Taylor, P., (2012). The role of edible mushrooms in health: Evaluation of the evidence, J. Func. Foods, 4, 687 –709.
- Singh, A. D., Abdullah, N. and Vikineswary, S. (2003). Optimization of extraction of bulk enzymes from spent mushroom compost. J. Chem. Technol. Biotechnol., 78(7), 743-752.
- Solak, M. H., Kalmis, E., Saglam, H. and Kalyoncu, F. (2006). Antimicrobial activity of two wild mushrooms Clitocybe alexandri (Gill.) Konr. and Rhizopogon roseolus (Corda) TM Fries collected from Turkey. Phytotherapy Res., 20(12), 1085-1087.
- Voběrková, S., Solčány, V., Vršanská, M. and Adam, V. (2018). Immobilization of ligninolytic enzymes from white-rot fungi in cross linked aggregates. Chemosphere, 202, 694-707.
Nemlendirme Sıvısı Olarak Kullanılan Alternatif Endüstriyel Atıkların Bazı Makrofungusların Misel Gelişimi Ve Ligninolitik Enzim Aktiviteleri Üzerine Etkisi
Abstract
Bu çalışmada endüstriyel üretim süreçlerinden açığa
çıkan farklı nitelikteki sıvı atıkların makrofungus üretiminde nemlendirme
sıvısı olarak kullanım olanakları araştırılmıştır. Bu amaçla kullanılan
nemlendirme sıvılarının beş farklı makrofungus türünün misel gelişimi ve
ligninolitik enzim aktivitesi üzerine etkisi belirlenmiştir. Çalışmada
kullanılan Pleurotus cornucopiae, Flammulina velutipes, Panus neostrigosus, Schizophyllum
commune ve Ganoderma lucidum kültürleri; Eskişehir
Osmangazi Üniversitesi Biyoloji Bölümü Makrofungus Kültür Koleksiyonundan
(OBCC) temin edilmiştir. En iyi misel gelişim ve enzim üretim koşullarının belirlenmesi amacıyla 4
farklı kompost materyali (meşe, kavak, pirinç, buğday) ikişerli
kombinasyonlarla 10 gram olacak şekilde karıştırılarak, tek ve karışım halinde
kullanılan 9 farklı nemlendirme sıvısıyla %70 oranında nemlendirilmiştir.
Pozitif kontrol olarak distile su kullanılmıştır. Her kompost örneğinin pH
değeri %1 alçı ve kireç ilavesi ile düzenlenmiştir. Saf fungal kültürlerden
çıkarılan 6 mm’lik misel diskleri nemli kompost karışımları içeren petrilerin
merkezine yerleştirilerek 28 °C’de inkübasyona bırakılmıştır. Araştırılan tüm fungus izolatlarının
çalışma kapsamında sorgulanan tüm ligninolitik enzimleri farklı seviyelerde
ürettiği belirlenmiştir. En yüksek mangan peroksidaz (MnP) aktivitesi Pleurotus
cornucopiae’de 103,64
u/L, en yüksek lakkaz ve mangan bağımsız peroksidaz (MnBP)
aktivitesi Ganoderma lucidum’ da sırasıyla 118,52 ve 66,86 u/L olarak
belirlenmiştir. En yüksek günlük koloni büyüme hızı ve misel gelişimi gösteren
izolat Schizophyllum commune olarak belirlenmiştir.
References
- Bayburt, C., Karaduman, A., Çelik, U. ve Yamaç, M. (2014). Farklı gelişim dönemlerinde Pleurotus ostreatus kompostundan ligninolitik enzim ekstraksiyonu için uygun yöntem seçimi. Mantar Dergisi, 5(2), 17 24.
- Boyle, C. D., Kropp, B. R. and Reid, I. D. (1992). Solubilization and mineralization of lignin by white rot fungi. Appl. Environ. Microbiol., 58(10), 3217-3224.
- Dönmez, G. (2002). Bioaccumulation of the reactive textile dyes by Candida tropicalis growing in molasses medium. Enzyme and Microbial Technol., 30(3), 363 366.
- Elisashvili, V., Kachlishvili, E. and Penninckx, M. (2008). Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes. J. Ind. Microbiol. Biotechnol., 35(11), 1531-1538.
- Kalmıs, E., Azbar, N., Yıldız, H. and Kalyoncu, F., (2008). Feasibility of using olive mill effluent (OME) as a wetting agent during the cultivation of oyster mushroom, Pleurotus ostreatus, on wheat straw. Bioresource Technol., 99 164–169.
- Koutrotsios, G., Larou, E., Mountzouris, K.C. and Zervakis, G.I. (2016). Detoxification of Olive Mill Wastewater and Bioconversion of Olive Crop Residues into High-Value-Added Biomass by the Choice Edible Mushroom Hericium erinaceus. Appl. Biochem. Biotechnol., 180,195–209.
- Leonowicz, A., Matuszewska, A., Luterek, J., Ziegenhagen, D., Wojtaś-Wasilewska, M., Cho, N. S. and Rogalski, J. (1999). Biodegradation of lignin by white rot fungi. Fungal Gen. Biol., 27(23), 175-185.
- Loss, E., Royer, A.R., Barreto-Rodrigues, M. and Barana, A.C., (2009). Use of maize wastewater for the cultivation of the Pleurotus spp. Mushroom and optimization of its biological efficiency. J. Hazard. Mat., 166, 1522–1525.
- Maciel, M. J. M. and Ribeiro, H. C. T. (2010). Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Electronic J Biotechnol., 13(6), 14-15.
- Martirani, L., Giardina, P., Marzullo, L. and Sannia, G. (1996). Reduction of phenol content and toxicity in olive oil mill waste waters with the ligninolytic fungus Pleurotus ostreatus. Water Res., 30(8), 1914-1918.
- Mohammad, N., Alam, M. Z., Kabbashi, N. A. and Ahsan, A. (2012). Effective composting of oil palm industrial waste by filamentous fungi: A review. Res. Conserv. Recycling, 58, 69-78.
- Naraian, R., Srivastava, J. and Garg, S.K. (2011). Influence of dairy spent wash (DSW) on different cultivation phases and yield response of two Pleurotus mushrooms. Ann. Microbiol., 61,853–862.
- Öztürk, A. ve Çopur, Ö. U. (2009). Mantar bileşenlerinin teröpatik etkileri. Bahçe, 38(1), 19-24.
- Pekşen, A. ve Günay, A. (2009). Kültür Mantarı (Agaricus bisporus (L.) Sing.) Yetiştiriciliğinde Çay Atığı ve Buğday Sapı Karışımından Hazırlanan Kompostların Kullanımı. Ekoloji Dergisi, 19(73), 48-54.
- Roupas, P., Keogh, J., Noakes, M., Margetts, C. and Taylor, P., (2012). The role of edible mushrooms in health: Evaluation of the evidence, J. Func. Foods, 4, 687 –709.
- Singh, A. D., Abdullah, N. and Vikineswary, S. (2003). Optimization of extraction of bulk enzymes from spent mushroom compost. J. Chem. Technol. Biotechnol., 78(7), 743-752.
- Solak, M. H., Kalmis, E., Saglam, H. and Kalyoncu, F. (2006). Antimicrobial activity of two wild mushrooms Clitocybe alexandri (Gill.) Konr. and Rhizopogon roseolus (Corda) TM Fries collected from Turkey. Phytotherapy Res., 20(12), 1085-1087.
- Voběrková, S., Solčány, V., Vršanská, M. and Adam, V. (2018). Immobilization of ligninolytic enzymes from white-rot fungi in cross linked aggregates. Chemosphere, 202, 694-707.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | XI. Türkiye Yemeklik Mantar Kongresi |
| Authors | |
| Publication Date | December 26, 2019 |
| Published in Issue | Year 2019 Volume: 10 Issue: 3 |
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