Abstract
This study was conducted to determine the allelopathic effect of Diplotaxis tenuifolia (L.) DC plant extracts, naturally distributed in Kırşehir Province, on some cultivated plants. The plants were collected at the flowering stage and dried in the shade. Doses of 5%, 10% and 20% were obtained from dry plant material by using pure water. 1 liter of methanol was added to 100 g dry plant material and left for 48 hours. After evaporating the methanol, the remaining substance was dissolved with DMSO to obtain doses of 150, 300 and 600 ppm. 2-layer filter paper was placed in 9 cm diameter petri dishes and the seeds of the test plants (barley, wheat, cress and grass) were counted as 25 pieces each. For control, 6 ml of pure (water + DMSO) and 6 ml of the obtained extracts were added to the prepared petri dishes, parafiltered and incubated at 24 °C for 1-3 weeks. At the end of the period, the seed germination number, root and shoot lengths of the test plants were determined. As a result, it was determined that plant water and methanol extracts did not have a statistically negative effect on cress seed germination, but inhibited seedling development compared to control. It, also, showed different effects at different levels on barley, wheat and grass seeds, depending on the dose and extract. Our study revealed that the Diplotaxis tenufolia plant, which is naturally distributed in many regions of our country, is known to be resistant to drought and is mostly mentioned in the literature for human nutrition, has an allelopathic effect on some cultivated plants.
References
- Abdalrahman KS, Güneş MG, Shomali N, İşgör BS, Yıldırım Ö (2018). Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity. Turk J Pharm Sci.,15(1):97-102.
- Alam SM, Islam E (2002). Effects of aqueous extract of leaf, stem and root of nettleleaf goosefoot and NaCl on germination and seedling growth of rice. Pak J Seed Technol 1: 47-52.
- Atta EM, Hashem AI, Ahmed AM, Elqosyd MS, Jasparse M, El‐Sharkaw ER (2011). Phytochemical studies on Diplotax isharra growing in Sinai. Euro J of Chem.;2:535-538.
- Cheng F, Cheng Z (2015). Research Progress on the use of plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy. Research Progress on the use of Plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy. Front. Plant Sci. 6:1020.
- Erik S (2012). Çok Yönlü Ruderal Bir Tür: Diplotaxis tenuıifolia (L) DC.A Multy Functional Rubbish Plant: Diplotaxis tenuifolia (L.) DC. Ankara Üniversitesi Çevrebilimleri Dergisi, 4 (1): 27-35.
- Giordano S, Molinaro A, Spagnuolo V, Muscariello L, Ferrara R, Cennamo G, Aliotta G (2005) In vitro allelopathic properties of wild rocket (Diplotaxis tenuifolia DC) extract and of its potential allelochemical S-glucopyranosyl thiohydroximate. Journal of Plant-Microbe Interactions 1, 51-60.
- Kadıoğlu I, Yanar Y (2004). Allelopathic effects of plant extracts against seed germination of some weeds. Assian Journal of Plant Sciences,3(4), 472-475
- Oueslatiab S, Ellilic A, Legaultb J, Pichetteb A, Ksouria R, Lachaalc M, Karray-Bouraoui N(2015) Phenolic content, antioxidant and anti-inflammatory activities of Tunisian Diplotaxis simplex (Brassicaceae). Nat Prod Res.;29:1189-1191.
- Qasem JR (2007). Allelopathic Activity of White Rocket [Diplotaxis erucoides (L.) DC.]. Allelopathy, Chapter 9, Page:25.
- Önen H (2003). Bazı bitkisel uçucu yağların biyoherbisidal etkileri, Türkiye Herboloji Dergisi, 6(1), 39-47.
- Özcan C (2015). Diplotaxis tenuifolia (Yabani Roka) bitkisinin fumigant beslemede kullanılabilirliğinin araştırılması, Doktora Tezi, Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü.
- Özdemir Ş (2007). Brassicaceae familyasından bazı bitkilere ait ekstraktların yabancı otlarla mücadelede biyo-herbisit olarak kullanılabilme olanaklarının araştırılması. (Yüksek Lisans Tezi). Mustafa kemal Üniversitesi. Fen Bilimleri Enstitüsü, Bitki Koruma Bölümü, Antakya.
- Rodriguez SA, Vela Gurovic MS, Mulet AC, Murray AP (2006). Diplotaxis tenuifolia (L.) DC., a source of a potentially antifungal essential oil containing nitrile. Biochemical Systematics and Ecology 34: 353-355.
- Rodríguez-Romero M, Godoy-Cancho B, Calha I.M, Passarinho JA, Moreira AC (2021). Allelopathic Effects of Three Herb Species on Phytophthora cinnamomi, a Pathogen Causing Severe Oak Decline in Mediterranean Wood Pastures. Forests, 12, 285
- Serim AT, Güzel NP, Türktemel İ (2015). Allelopatik bitki ekstraktları ile herbisitlerin beraber kullanımı. Derim, 32 (2): 225-236.
- Yılar M, Bayar Y, Abacı Bayar AA, Genc N (2020). Chemical composition of the essential oil of Salvia bracteata Banks and the biological activity of its extracts: antioxidant, total phenolic, total flavonoid, antifungal and allelopathic effects. Botanica Serbica 44 (1): 71-79.
Diplotaxis tenuifolia (L.) DC. ( Türpenk ) Bitkisinin Bazı Kültür Bitkileri Üzerine Allelopatik Etkisi
Abstract
Kırşehir İlinde doğal yayılış gösteren Diplotaxis tenuifolia (L.) DC. bitki ekstraktlarının bazı kültür bitkileri üzerine allelopatik etkisinin belirlenmesi amacıyla yürütülen bu çalışmada bitkiler çiçeklenme aşamasında toplanmış ve gölgede kurutulmuşlardır. Kuru bitki materyalinden saf su kullanılarak %5,%10 ve %20’lik dozlar elde edilmiştir. 100gr kuru bitki materyali üzerine 1 litre methanol ilave edilerek 48 saat bekletilmiştir. Methaol uçurulduktan sonra geriye kalan madde DMSO ile çözülerek 150, 300 ve 600 ppm dozlar elde edilmiştir. 9 cm çaplı petri kaplarına 2 katlı filtre kağıdı yerleştirilmiş ve test bitkilerine ait tohumlar 25’er adet sayılarak konmuştur. Hazırlanmış petri kapları kontrol amaçlı 6 ml saf (su+DMSO) ve elde edilen ekstraktlardan 6 ml ilave edilmiş, parafillenerek 24 oC de 1-3 hafta süre ile inkübasyona bırakılmıştır. Süre sonunda test bitkilerinin tohum çimlenme sayısı, kök ve sürgün uzunlukları belirlenmiştir. Sonuç olarak bitki su ve methanol ekstraktları tere tohum çimlenmesi üzerine istatistiki olarak olumsuz bir etki göstermediği ancak fide gelişimini kontrole kıyasla engellediği belirlenmiştir. Yine arpa, buğday ve çim tohumlarında da doza ve ekstrakta bağlı olarak farklı düzeylerde farklı etkiler göstermiştir. Bu çalışma ile, ülkemizin birçok yöresinde doğal yayılış gösteren, kuraklığa dayanıklı olduğu bilinen ve daha çok insan beslenmesi yönünde litaratürde yer alan Diplotaxis tenufolia bitkisinin bazı kültür bitkileri üzerine allelopatik etkisi olduğu ortaya konmuştur.
References
- Abdalrahman KS, Güneş MG, Shomali N, İşgör BS, Yıldırım Ö (2018). Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity. Turk J Pharm Sci.,15(1):97-102.
- Alam SM, Islam E (2002). Effects of aqueous extract of leaf, stem and root of nettleleaf goosefoot and NaCl on germination and seedling growth of rice. Pak J Seed Technol 1: 47-52.
- Atta EM, Hashem AI, Ahmed AM, Elqosyd MS, Jasparse M, El‐Sharkaw ER (2011). Phytochemical studies on Diplotax isharra growing in Sinai. Euro J of Chem.;2:535-538.
- Cheng F, Cheng Z (2015). Research Progress on the use of plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy. Research Progress on the use of Plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy. Front. Plant Sci. 6:1020.
- Erik S (2012). Çok Yönlü Ruderal Bir Tür: Diplotaxis tenuıifolia (L) DC.A Multy Functional Rubbish Plant: Diplotaxis tenuifolia (L.) DC. Ankara Üniversitesi Çevrebilimleri Dergisi, 4 (1): 27-35.
- Giordano S, Molinaro A, Spagnuolo V, Muscariello L, Ferrara R, Cennamo G, Aliotta G (2005) In vitro allelopathic properties of wild rocket (Diplotaxis tenuifolia DC) extract and of its potential allelochemical S-glucopyranosyl thiohydroximate. Journal of Plant-Microbe Interactions 1, 51-60.
- Kadıoğlu I, Yanar Y (2004). Allelopathic effects of plant extracts against seed germination of some weeds. Assian Journal of Plant Sciences,3(4), 472-475
- Oueslatiab S, Ellilic A, Legaultb J, Pichetteb A, Ksouria R, Lachaalc M, Karray-Bouraoui N(2015) Phenolic content, antioxidant and anti-inflammatory activities of Tunisian Diplotaxis simplex (Brassicaceae). Nat Prod Res.;29:1189-1191.
- Qasem JR (2007). Allelopathic Activity of White Rocket [Diplotaxis erucoides (L.) DC.]. Allelopathy, Chapter 9, Page:25.
- Önen H (2003). Bazı bitkisel uçucu yağların biyoherbisidal etkileri, Türkiye Herboloji Dergisi, 6(1), 39-47.
- Özcan C (2015). Diplotaxis tenuifolia (Yabani Roka) bitkisinin fumigant beslemede kullanılabilirliğinin araştırılması, Doktora Tezi, Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü.
- Özdemir Ş (2007). Brassicaceae familyasından bazı bitkilere ait ekstraktların yabancı otlarla mücadelede biyo-herbisit olarak kullanılabilme olanaklarının araştırılması. (Yüksek Lisans Tezi). Mustafa kemal Üniversitesi. Fen Bilimleri Enstitüsü, Bitki Koruma Bölümü, Antakya.
- Rodriguez SA, Vela Gurovic MS, Mulet AC, Murray AP (2006). Diplotaxis tenuifolia (L.) DC., a source of a potentially antifungal essential oil containing nitrile. Biochemical Systematics and Ecology 34: 353-355.
- Rodríguez-Romero M, Godoy-Cancho B, Calha I.M, Passarinho JA, Moreira AC (2021). Allelopathic Effects of Three Herb Species on Phytophthora cinnamomi, a Pathogen Causing Severe Oak Decline in Mediterranean Wood Pastures. Forests, 12, 285
- Serim AT, Güzel NP, Türktemel İ (2015). Allelopatik bitki ekstraktları ile herbisitlerin beraber kullanımı. Derim, 32 (2): 225-236.
- Yılar M, Bayar Y, Abacı Bayar AA, Genc N (2020). Chemical composition of the essential oil of Salvia bracteata Banks and the biological activity of its extracts: antioxidant, total phenolic, total flavonoid, antifungal and allelopathic effects. Botanica Serbica 44 (1): 71-79.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Agricultural Engineering (Other) |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 31, 2023 |
| Submission Date | November 27, 2023 |
| Acceptance Date | December 20, 2023 |
| Published in Issue | Year 2023 Volume: 3 Issue: 2 |
